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DNA uptake during bacterial transformation

Nature Reviews Microbiology, 2004
Naturally competent bacteria are able to take up exogenous DNA and undergo genetic transformation. The transport of DNA from the extracellular milieu into the cytoplasm is a complex process, and requires proteins that are related to those involved in the assembly of type IV pili and type II secretion systems, as well as a DNA translocase complex at the
Inês, Chen, David, Dubnau
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Bacterial Transformation

2003
If one consults any genetics textbook written before the mid-1970s, one will find much on the transformation of such bacterial species as Bacillus subtilis, but no mention of Escherichia coli. The method now most generally used for introducing DNA into E.
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Bacterial transformation of terpenoids

Russian Chemical Reviews, 2014
Data on the bacterial transformation of terpenoids published in the literature in the past decade are analyzed. Possible pathways for chemo-, regio- and stereoselective modifications of terpenoids are discussed. Considerable attention is given to new technological approaches to the synthesis of terpenoid derivatives suitable for the use in the perfume ...
V V Grishko   +2 more
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"Steady-state" competence for bacterial transformation

Canadian Journal of Microbiology, 1968
The cells of Bacillus subtilis, grown in minimal media, are known to become competent for transformation for a short period at a specific phase of ceil growth. In the present work the cells (strain 168 ind−) were grown in continuous culture (chemostat; glucose limiting, generation time 4 h, 37 °C).
H H, Eichhorn, S, Arikawa, S, Zamenhof
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Bacterial colony counting using distance transform

International Journal of Bio-Medical Computing, 1995
A distance-transform based technique is presented for the segmentation of monochrome images of colonies grown on membrane filters. This is used to count the number of Escherichia coli in a given water sample, which is used as a parameter for determining water quality. The result is compared with fuzzy c-means clustering approach.
D P, Mukherjee   +3 more
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Bacterial Transformations of Benzothiophene and Methylbenzothiophenes

Environmental Science & Technology, 1994
The abilities of three Pseudomonas strains transform each of the six isomers of methylbenzothiophene was studied. These compounds would not support growth but were biotransformed in a cell suspension of one of the violates and in cultures of the other two isolates while they grew on 1-methylnaphthalene or glucose.
K G, Kropp   +3 more
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Bacterial transformation using micro-shock waves

Analytical Biochemistry, 2011
Shock waves are one of the most competent mechanisms of energy dissipation observed in nature. We have developed a novel device to generate controlled micro-shock waves using an explosive-coated polymer tube. In this study, we harnessed these controlled micro-shock waves to develop a unique bacterial transformation method. The conditions were optimized
Prakash, Divya G   +3 more
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Bacterial-induced transformation

Science Signaling, 2015
Salmonella infection triggers signaling events that increase the probability of developing cancer in hosts that are genetically predisposed to transformation.
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Genomic Insights into Bacterial DMSP Transformations

Annual Review of Marine Science, 2012
Genomic and functional genomic methods applied to both model organisms and natural communities have rapidly advanced understanding of bacterial dimethylsulfoniopropionate (DMSP) degradation in the ocean. The genes for the two main pathways in bacterial degradation, routing DMSP to distinctly different biogeochemical fates, have recently been identified.
Mary Ann, Moran   +3 more
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Mechanism of Bacterial Transformation and Transfection

1974
Publisher Summary This chapter discusses that in spite of the large amount of information on the physiology of the competent cell, including the production of competence factors in a number of transformation systems and an additional protein that must be synthesized to permit irreversible binding of DNA by D.
N K, Notani, J K, Setlow
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