Results 61 to 70 of about 453,232 (262)

Genetic transformation of the dinoflagellate chloroplast

eLife, 2019
Coral reefs are some of the most important and ecologically diverse marine environments. At the base of the reef ecosystem are dinoflagellate algae, which live symbiotically within coral cells.
Isabel C Nimmo, Adrian C Barbrook, Imen Lassadi, Jit Ern Chen, Katrin Geisler, Alison G Smith, Manuel Aranda, Saul Purton, Ross F Waller, R Ellen R Nisbet, Christopher J Howe   +10 more
doaj   +1 more source

Organ‐specific redox imbalances in spinal muscular atrophy mice are partially rescued by SMN antisense oligonucleotides

FEBS Letters, EarlyView.
We identified a systemic, progressive loss of protein S‐glutathionylation—detected by nonreducing western blotting—alongside dysregulation of glutathione‐cycle enzymes in both neuronal and peripheral tissues of Taiwanese SMA mice. These alterations were partially rescued by SMN antisense oligonucleotide therapy, revealing persistent redox imbalance as ...
Sofia Vrettou, Brunhilde Wirth
wiley   +1 more source

Research progress of nanomaterials in plant genetic transformation

上海师范大学学报. 自然科学版
Plant genetic transformation is crucial for improving quality of crop straits, cultivating new varieties with high yield, improved quality, and multi-resistance, thereby reducing the use of pesticides and fertilizers.
YANG Demin, CAO Tingting, LYU Min, CHEN Nan   +3 more
doaj   +1 more source

The planar cell polarity protein Vangl2 interacts with the PDZ‐domains of Scribble but not with a unique PDZ‐like domain in Inturned

FEBS Letters, EarlyView.
Structural and biochemical characterisations show that the planar cell polarity (PCP) protein Inturned harbours a unique PDZ‐like domain that does not bind canonical PDZ‐binding motifs (PBMs) like that of another PCP protein Vangl2. In contrast, the apical‐basal polarity protein Scribble contains four PDZ domains that bind Vangl2, but one PDZ domain ...
Stephan Wilmes, Jan Brysch, Carmen Gelze, Lilli Meier, Daniel Kümmel   +4 more
wiley   +1 more source

Structural insights into an engineered feruloyl esterase with improved MHET degrading properties

FEBS Letters, EarlyView.
A feruloyl esterase was engineered to mimic key features of MHETase, enhancing the degradation of PET oligomers. Structural and computational analysis reveal how a point mutation stabilizes the active site and reshapes the binding cleft, expading substrate scope.
Panagiota Karampa, Konstantinos Makryniotis, Theofani‐Iosifina Sousani, Evangelos Topakas, Vangelis Daskalakis, Maria Dimarogona   +5 more
wiley   +1 more source

Construction of a new type of multi-gene plant transformation vector and genetic transformation of tobacco

Biologia Plantarum, 2017
A plasmid and two isocaudamer systems, namely, NotI/Bsp120I and SpeI/XbaI/NheI, were used to construct a new type of multi-gene plant transformation vector system.
Y. Dong, Y. C. Ren, M. S. Yang, J. Zhang, T. Qiu, H. L. Cui   +5 more
doaj   +1 more source

Gut microbiome and aging—A dynamic interplay of microbes, metabolites, and the immune system

FEBS Letters, EarlyView.
Age‐dependent shifts in microbial communities engender shifts in microbial metabolite profiles. These in turn drive shifts in barrier surface permeability of the gut and brain and induce immune activation. When paired with preexisting age‐related chronic inflammation this increases the risk of neuroinflammation and neurodegenerative diseases.
Aaron Mehl, Eran Blacher
wiley   +1 more source

Advances in the Identification of Novel Urinary Biomarkers for Diabetic Kidney Disease

Journal of Diabetes Research
Diabetic kidney disease (DKD) is a major microvascular complication of diabetes and remains one of the leading causes of end-stage renal disease, significantly affecting patients' survival rates and quality of life.
Yujie Jin, Yan Ma, Yan Yao, Mengru Wang, Chunchen Ni, Shujuan Shang, Yongxin Cui, Xinyu Wang, Ye Ling, Yumeng Sun, Qirui Pei, Shiqiang Liu, Lizhuo Wang, Jialin Gao   +13 more
doaj   +1 more source

Review and Validation of Plant Gene Function Research Methods Bypassing Tissue Culture

Agronomy
The genetic transformation of plants has provided fundamental insights into plant biology. However, the genetic transformation systems for most horticultural plants remain incomplete.
Huanghuang Jiang, Suya Huang, Jiayi Wang, Deming Sun, Min Wang, Su Lin, Jinhua Liu, Kai Xu, Bei Liu, Haijie Ma   +9 more
doaj   +1 more source

Valosin‐containing protein counteracts ATP‐driven dissolution of FUS condensates through its ATPase activity in vitro

FEBS Letters, EarlyView.
Biomolecular condensates formed by fused in sarcoma (FUS) are dissolved by high ATP concentrations yet persist in cells. Using a reconstituted system, we demonstrate that valosin‐containing protein (VCP), an AAA+ ATPase, counteracts ATP‐driven dissolution of FUS condensates through its D2 ATPase activity.
Hitomi Kimura, Shin‐ichi Tate, Kyota Yasuda   +2 more
wiley   +1 more source