Results 81 to 90 of about 3,292,740 (304)

Development of human monoclonal antibodies against TARM1 by yeast display

FEBS Open Bio, EarlyView.
Human monoclonal antibodies against TARM1 are generated by yeast display‐guided selection. These antibodies bind to soluble and cell‐surface forms of TARM1. Also, these antibodies exhibit agonistic activity in the NFAT‐GFP reporter assay, indicating that TARM1 signaling can be functionally modulated by antibodies and suggesting TARM1 as a potential ...
Rikio Yabe, Mayumi Saeki, Masaaki Hashiguchi, Sayaka Ono, Kazuhisa Aoki, Hidetaka Tanno   +5 more
wiley   +1 more source

Screening and epitope characterization of Nidogen‐2‐specific nanobodies

FEBS Open Bio, EarlyView.
Camel immunization and phage display were employed to generate high‐affinity VHH nanobodies against Nidogen‐2. After library construction, biopanning, ELISA screening, sequencing, and recombinant expression, selected nanobodies were purified and characterized, leading to the preliminary exploration of a nanobody‐based sandwich ELISA for specific ...
Jianchuan Wen, Qianqian Cui, Zhongyun Lan, Yingjun Wang, Shuaiying Zhao, Wenxuan Feng, Yunfeng Liu, Qiting Huang, Dongna Zhang, Jianfeng Xu   +9 more
wiley   +1 more source

Comparative study on the effects of Nb and V on the nucleation and growth of pearlite in high-carbon steels using in-situ method

Journal of Materials Research and Technology
The formation of pearlite in three high-carbon steels with different Nb and V additions was directly observed using a high-temperature laser scanning confocal microscope. The number densities and growth rates of pearlite nodules, and the overall pearlite
Keduo Yu, Mingxing Zhou, Min Zhu, Houxin Wang, Zhenye Chen, Weifan Yin, Xue Su, Junyu Tian, Guang Xu   +8 more
doaj   +1 more source

KINETICS OF ORDER-DISORDER TRANSFORMATIONS

Le Journal de Physique Colloques, 1977
Relaxation of order (both long range and short range) in bcc binary alloys is worked out theoretically based on the vacancy mechanism of atomic migration using the pair approximation of the Path Probability method. Concepts of relaxation times and relaxation modes in states far from the equilibrium are examined. A peculiar isothermal relaxation process
Sato, H., Gschwend, K., Kikuchi, R.
openaire   +2 more sources

Proteasomal degradation of intracellularly expressed Amblyomin‐X limits suicide gene therapy potential in melanoma cells

FEBS Open Bio, EarlyView.
This study explores the feasibility of expressing the antitumoral protein Amblyomin‐X through a suicide gene therapy approach and investigates its intracellular fate after gene delivery. Although the gene is efficiently expressed, melanoma cells rapidly degrade the Amblyomin‐X protein via proteasome activity.
Victor Dal Posolo Cinel, Carlos DeOcesano Pereira, Aline Ramos Maia Lobba, Melissa Regina Fessel, Ana Marisa Chudzinski‐Tavassi   +4 more
wiley   +1 more source

Effect of prior martensite on bainitic transformation of high Si hypereutectoid steel

Journal of Materials Research and Technology
The paper discusses the effect of prior martensite on the bainitic transformation of a high Si hypereutectoid steel. The research uses a combination of experimental techniques, high-resolution dilatometry and X-ray diffraction analysis, and theoretical ...
Carlos Garcia-Mateo, Thomas Sourmail, Amandine Philippot, Lucia Morales-Rivas, Jose A. Jimenez   +4 more
doaj   +1 more source

The Effect of Initial Structure on Phase Transformation in Continuous Heating of a TA15 Titanium Alloy

Metals, 2017
The effect of initial structure on phase evolution in continuous heating of a near-α TA15 titanium alloy (Ti-6Al-2Zr-1Mo-1V) was experimentally investigated. To this end; three microstructures were obtained by multiple heat treatment: I-bimodal structure
Xiaoguang Fan, Qi Li, Anming Zhao, Yuguo Shi, Wenjia Mei   +4 more
doaj   +1 more source

Rapid screening of staphylokinase protein variants using an unpurified cell‐free expression system

FEBS Open Bio, EarlyView.
An unpurified cell‐free protein synthesis (CFPS) platform enables rapid functional screening of staphylokinase variants. Direct plasminogen‐activation assays performed in microplate format provide real‐time activity readouts, allowing rapid identification and ranking of variants with improved or reduced fibrinolytic activity without protein ...
Maria Tomková, Veronika Hovanová, Jiri Damborsky, Erik Sedlák   +3 more
wiley   +1 more source

Tensorial stress-plastic strain fields in α - ω Zr mixture, transformation kinetics, and friction in diamond-anvil cell. [PDF]

Nat Commun, 2023
Levitas VI, Dhar A, Pandey KK.
europepmc   +1 more source

YIPFα1A expression is regulated by multilayered molecular mechanisms

FEBS Open Bio, EarlyView.
YIPFα1A, a five‐pass Golgi protein, is regulated at multiple layers. (1) Rare‐codon enrichment drives translation‐coupled mRNA decay. (2) A proximal 3′‐UTR element stabilizes mRNA. (3) A distal 3′‐UTR element included by alternate poly(A) site usage represses translation, which can be overridden by the proximal 3′‐UTR element.
Tokio Takaji, Yurika Nakanishi, Nobuhiro Nakamura   +2 more
wiley   +1 more source