Results 61 to 70 of about 125,176 (265)

Automated FRAP microscopy for high‐throughput analysis of protein dynamics in chromatin organization and transcription

FEBS Open Bio, EarlyView.
RoboMic is an automated confocal microscopy pipeline for high‐throughput functional imaging in living cells. Demonstrated with fluorescence recovery after photobleaching (FRAP), it integrates AI‐driven nuclear segmentation, ROI selection, bleaching, and analysis.
Selçuk Yavuz, Bart Geverts, Johan A. Slotman, Andrea Sacchetti, Stefan Prekovic, Martin E. van Royen, Adriaan B. Houtsmuller   +6 more
wiley   +1 more source

Incompleteness of bainite transformation in quenched and tempered steel under continuous cooling conditions

Journal of Materials Research and Technology, 2020
The austenitic transformation kinetics of quenched and tempered 22CrMnNiMo steel was investigated by dilatometric measurement at different cooling rates of 0.1∼20 K/s.
F.-C. An, S.-X. Zhao, X.-K. Xue, J.-J. Wang, G. Yuan, C.-M. Liu   +5 more
doaj   +1 more source

Polarization‐resolved femtosecond Vis/IR spectroscopy tailored for resolving weak signals in biological samples using minimal sample volume

FEBS Open Bio, EarlyView.
Unique biological samples, such as site‐specific mutant proteins, are available only in limited quantities. Here, we present a polarization‐resolved transient infrared spectroscopy setup with referencing to improve signal‐to‐noise tailored towards tracing small signals. We provide an overview of characterizing the excitation conditions for polarization‐
Clark Zahn, Karsten Heyne
wiley   +1 more source

Stability of metastable phases: pathway-dependent transformation kinetics of the γ phase in eutectic AuGe

Materials & Design
The transformation kinetics of metastable phases into more stable phases with simultaneous variation of the phase composition is studied in metallic systems using the binary Au72Ge28 eutectic alloy as model system.
Jürgen E.K. Schawe, Štefan T. Stanko, Marc-Georg Willinger, Jörg F. Löffler   +3 more
doaj   +1 more source

Kinetics of phase transformations

, 2015
In this thesis, the kinetics of heterogeneous solid-state phase transformations in different prototype experimental systems are investigated with focus on the development of new strategies for kinetic modelling using mean-field kinetic models. Topics cover the interrelation between the kinetic model description and the amount of available experimental ...
openaire   +2 more sources

A light‐triggered Time‐Resolved X‐ray Solution Scattering (TR‐XSS) workflow with application to protein conformational dynamics

FEBS Open Bio, EarlyView.
Time‐resolved X‐ray solution scattering captures how proteins change shape in real time under near‐native conditions. This article presents a practical workflow for light‐triggered TR‐XSS experiments, from data collection to structural refinement. Using a calcium‐transporting membrane protein as an example, the approach can be broadly applied to study ...
Fatemeh Sabzian‐Molaei, Fredrik Orädd, Konstantinos Magkakis, Magnus Andersson   +3 more
wiley   +1 more source

Effect of Microstructure on Stress Dependence of Transformation Induced Plasticity in TRIP800 Low-alloy Multiphase Steels

Iranian Journal of Materials Science and Engineering, 2020
An experimental–numerical methodology was used in order to study the microstructural effects on stress state dependency of martensitic transformation kinetics in two different TRIP800 low alloy multiphase steels.
F. Hosseinabadi, A. Rezaee-Bazzaz, M. Mazinani, B. Mohammad Sadeghi   +3 more
doaj  

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

FEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli, Mohammad Nour Alsamsam, Ugnė Bagdonaitė, Van Truc Vu, Chun‐Jen Huang, Polina Kuzhir, Mindaugas Zaremba, Aurimas Kopūstas, Marijonas Tutkus   +8 more
wiley   +1 more source

YIPFα1A expression is regulated by multilayered molecular mechanisms

FEBS Open Bio, EarlyView.
YIPFα1A, a five‐pass Golgi protein, is regulated at multiple layers. (1) Rare‐codon enrichment drives translation‐coupled mRNA decay. (2) A proximal 3′‐UTR element stabilizes mRNA. (3) A distal 3′‐UTR element included by alternate poly(A) site usage represses translation, which can be overridden by the proximal 3′‐UTR element.
Tokio Takaji, Yurika Nakanishi, Nobuhiro Nakamura   +2 more
wiley   +1 more source

Comparative study on the effects of Nb and V on the nucleation and growth of pearlite in high-carbon steels using in-situ method

Journal of Materials Research and Technology
The formation of pearlite in three high-carbon steels with different Nb and V additions was directly observed using a high-temperature laser scanning confocal microscope. The number densities and growth rates of pearlite nodules, and the overall pearlite
Keduo Yu, Mingxing Zhou, Min Zhu, Houxin Wang, Zhenye Chen, Weifan Yin, Xue Su, Junyu Tian, Guang Xu   +8 more
doaj   +1 more source