Results 111 to 120 of about 2,123,374 (298)

Polarization‐resolved femtosecond Vis/IR spectroscopy tailored for resolving weak signals in biological samples using minimal sample volume

open access: yesFEBS Open Bio, EarlyView.
Unique biological samples, such as site‐specific mutant proteins, are available only in limited quantities. Here, we present a polarization‐resolved transient infrared spectroscopy setup with referencing to improve signal‐to‐noise tailored towards tracing small signals. We provide an overview of characterizing the excitation conditions for polarization‐
Clark Zahn, Karsten Heyne
wiley   +1 more source

Closed-form and iterative weighted least squares solutions of Helmert transformation parameters

open access: yesJournal of Geodetic Science, 2013
The Helmert transformation is the most common transformation between different geodetic systems. In 2-D, in contrast to higher dimensions, it is a well-known procedure how to determine the 4 transformation parameters in a closed form.
Sjöberg L.E.
doaj   +1 more source

Study of the Effect of Hot Rolling Processing Parameters on the Variability of HSLA Steels [PDF]

open access: yes, 2002
The effect of different hot mill processing parameters and their influence on the variability of mechanical properties of HSLA steels has been studied.
Garcia Gonzalez, Jose Enrique
core   +1 more source

Multi‐omics and low‐input proteomics profiling reveals dynamic regulation driving pluripotency initiation in early mouse embryos

open access: yesFEBS Open Bio, EarlyView.
Mouse pre‐implantation development involves a transition from totipotency to pluripotency. Integrating transcriptomics, epigenetic profiling, low‐input proteomics and functional assays, we show that eight‐cell embryos retain residual totipotency features, whereas cytoskeletal remodeling regulated by the ubiquitin‐proteasome system drives progression ...
Wanqiong Li   +8 more
wiley   +1 more source

From the help desk: Polynomial distributed lag models [PDF]

open access: yes
Polynomial distributed lag models (PDLs) are finite-order distributed lag models with the impulse-response function constrained to lie on a polynomial of known degree.
Allen McDowell
core   +1 more source

Meta‐analysis fails to show any correlation between protein abundance and ubiquitination changes

open access: yesFEBS Open Bio, EarlyView.
We analyzed over 50 published proteomics datasets to explore the relationship between protein levels and ubiquitination changes across multiple experimental conditions and biological systems. Although ubiquitination is often associated with protein degradation, our analysis shows that changes in ubiquitination do not globally correlate with changes in ...
Nerea Osinalde   +3 more
wiley   +1 more source

GPS and LiDAR optimizing transformation parameters for localization in autonomous vehicles

open access: yesEgyptian Journal of Remote Sensing and Space Sciences
Accurate localization is necessary for autonomous vehicles, with the demand for the correct fusion of Global Navigation Satellite System (GNSS) and Light Detection and Ranging (LiDAR) data. Existing static transformation parameter optimization methods do
Sundoss ALMahadeen
doaj   +1 more source

A light‐triggered Time‐Resolved X‐ray Solution Scattering (TR‐XSS) workflow with application to protein conformational dynamics

open access: yesFEBS Open Bio, EarlyView.
Time‐resolved X‐ray solution scattering captures how proteins change shape in real time under near‐native conditions. This article presents a practical workflow for light‐triggered TR‐XSS experiments, from data collection to structural refinement. Using a calcium‐transporting membrane protein as an example, the approach can be broadly applied to study ...
Fatemeh Sabzian‐Molaei   +3 more
wiley   +1 more source

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

open access: yesFEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli   +8 more
wiley   +1 more source

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