The pH‐sensitive His6‐SWNTs, which is functionalized with oligohistidine, can deliver STTM396 molecules into callus cells. The STTM396–SWNT complex treatments enhance shoot regeneration efficiency by regulating the miR396‐GRF module in Arabidopsis and tomato calli.
Yeong Yeop Jeong +7 more
wiley +1 more source
Gene up-regulation by DNA demethylation in 35S-gshI-transgenic poplars (Populus x canescens) [PDF]
Gene expression levels of transgene 35S-gshI (γ-glutamylcysteine synthetase) cloned from E. coli, and the endogenous gene gsh1 of poplar (Populus x canescens) were upregulated by the DNA demethylating agent DHAC (5,6-dihydro-5'-azacytidine ...
Bittsánszky, András +7 more
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With many years of experience and in-depth knowledge, Creative Bioarray can use fluorescence in situ hybridization (FISH) to confirm the integration of transgenes and determine their chromosome band location. We guarantee the speed, quality and cost of our service.
openaire +2 more sources
Generation of transgene-free induced pluripotent mouse stem cells by the piggyBac transposon
Induced pluripotent stem cells (iPSCs) have been generated from somatic cells by transgenic expression of Oct4 (Pou5f1), Sox2, Klf4 and Myc. A major difficulty in the application of this technology for regenerative medicine, however, is the delivery of ...
K. Yusa, R. Rad, J. Takeda, A. Bradley
semanticscholar +1 more source
Granular Hydrogels as Modular Biomaterials: From Structural Design to Biological Responses
Granular hydrogels are now emerging as promising biomaterials due to their inherent microporousity, injectability, and modularity. They have shown improvements in cell viability and migration, cellular/tissue infiltration, host tissue integration, mitigated foreign body response, and tissue regeneration.
Asmasadat Vaziri +6 more
wiley +1 more source
Integrated DNA walking system to characterize a broad spectrum of GMOs in food/feed matrices [PDF]
Background: In order to provide a system fully integrated with qPCR screening, usually used in GMO routine analysis, as well as being able to detect, characterize and identify a broad spectrum of GMOs in food/feed matrices, two bidirectional DNA walking ...
De Loose, Marc +6 more
core +6 more sources
The Effects of Transcription Directions of Transgenes and the gypsy Insulators on the Transcript Levels of Transgenes in Transgenic Arabidopsis [PDF]
AbstractManipulation of a single abiotic stress-related gene could improve plant performance under abiotic stress conditions. To simultaneously increase plant tolerance to multiple stresses, it is usually required to overexpress two (or more) genes in transgenic plants.
Jiang, Weijia +8 more
openaire +2 more sources
Magnetic Bioprinting and Actuation of Stretchable Muscle Tissue
Human and murine myoblasts labeled with iron oxide nanoparticles are magnetically bioprinted into wrench‐shaped tissues, which are then anchored between two magnetic needles. Mechanical stretching of these tissues enhances both their maturation and functional performance. Abstract Engineering tissues with precise, long‐lasting shapes and the capability
Noam Demri +6 more
wiley +1 more source
Patient-derived tumoroids and proteomic signatures: tools for early drug discovery
Onco-virotherapy is an emergent treatment for cancer based on viral vectors. The therapeutic activity is based on two different mechanisms including tumor-specific oncolysis and immunostimulatory properties.
Hélène Lê +11 more
doaj +1 more source
A nitric oxide synthase transgene ameliorates muscular dystrophy in mdx mice. [PDF]
Dystrophin-deficient muscles experience large reductions in expression of nitric oxide synthase (NOS), which suggests that NO deficiency may influence the dystrophic pathology.
Spencer, MJ, Tidball, JG, Wehling, M
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