Results 141 to 150 of about 361,542 (380)

Global Screening of Antiviral Genes that Suppress Baculovirus Transgene Expression in Mammalian Cells

Molecular Therapy: Methods & Clinical Development, 2017
Although baculovirus has been used as a safe and convenient gene delivery vector in mammalian cells, baculovirus-mediated transgene expression is less effective in various mammalian cell lines.
Chia-Hung Wang, Nenavath Gopal Naik, Lin-Li Liao, Sung-Chan Wei, Yu-Chan Chao   +4 more
doaj   +1 more source

EGR Proteins Mediate Interferon‐Independent Anti‐HSV‐1 Responses Through Viral and Host Targets

Advanced Science, EarlyView.
Early antiviral responses are typically mediated by interferons. However, during HSV‐1 infection, host early growth response (Egr) genes, which are not interferon‐stimulated genes, are quickly induced by viral protein ICP0. EGR proteins, in turn, suppress viral lytic infection by activating viral latency‐associated (LAT) and host immune regulatory ...
Shuaishuai Wang, Fujun Hou, Xunuo Jin, Yuhang Xiang, Dongli Pan   +4 more
wiley   +1 more source

Adeno‐associated virus serotype 2 capsid variants for improved liver‐directed gene therapy

Hepatology, EarlyView., 2022
Abstract Background and Aims Current liver‐directed gene therapies look for adeno‐associated virus (AAV) vectors with improved efficacy. With this background, capsid engineering is explored. Whereas shuffled capsid library screenings have resulted in potent liver targeting variants with one first vector in human clinical trials, modifying natural ...
Nadja Meumann, Marti Cabanes‐Creus, Moritz Ertelt, Renina Gale Navarro, Julie Lucifora, Qinggong Yuan, Karin Nien‐Huber, Ahmed Abdelrahman, Xuan‐Khang Vu, Liang Zhang, Ann‐Christin Franke, Christian Schmithals, Albrecht Piiper, Annabelle Vogt, Maria Gonzalez‐Carmona, Jochen T. Frueh, Evelyn Ullrich, Philip Meuleman, Steven R. Talbot, Margarete Odenthal, Michael Ott, Erhard Seifried, Clara T. Schoeder, Joachim Schwäble, Leszek Lisowski, Hildegard Büning   +25 more
wiley   +1 more source

Effects of overexpression of jasmonic acid biosynthesis genes on nicotine accumulation in tobacco

Plant Direct, 2018
Nicotine is naturally synthesized in tobacco roots and accumulates in leaves as a defense compound against herbivory attack. Nicotine biosynthesis pathway has been extensively studied with major genes and enzymes being isolated and functionally ...
Hongxia Chen, Bingwu Wang, Sisi Geng, Consuelo Arellano, Sixue Chen, Rongda Qu   +5 more
doaj   +1 more source

Enhanced Transgene Expression from Recombinant Single-Stranded D-Sequence-Substituted Adeno-Associated Virus Vectors in Human Cell Lines In Vitro and in Murine Hepatocytes In Vivo [PDF]

, 2014
Chen Ling, Yuan Wang, Yuan Lu, Lina Wang, Giridhara R. Jayandharan, George Aslanidi, Baozheng Li, Binbin Cheng, Wenqin Ma, Thomas B. Lentz, Changquan Ling, Xiao Xiao, R. Jude Samulski, Nicholas Muzyczka, Arun Srivastava   +14 more
openalex   +1 more source

Polyethylenimine but Not Cationic Lipids Promotes Transgene Delivery to the Nucleus in Mammalian Cells*

Journal of Biological Chemistry, 1998
The β-galactosidase reporter gene, either free or complexed with various cationic vectors, was microinjected into mammalian cells. Cationic lipids but not polyethylenimine or polylysine prevent transgene expression when complexes are injected in the ...
Hélène Pollard, J. Remy, G. Loussouarn, S. Demolombe, J. Behr, D. Escande   +5 more
semanticscholar   +1 more source

Disrupting CSPG‐Driven Microglia–Astrocyte Crosstalk Enables Scar‐Free Repair in Spinal Cord Injury

Advanced Science, EarlyView.
This study identifies CSPGs as key drivers of glial scar maturation after spinal cord injury by reprogramming microglial metabolism and inducing astrocyte fibrosis. To address this, a reactive oxygen species‐responsive, reactive astrocyte‐targeted ChABC gene delivery system is designed to locally degrade CSPGs, precisely disrupt maladaptive glial ...
Yufei Zheng, Zhaowei Zhang, Zezhou Fu, Qingqing Wang, Siwen Zhang, Tingyu Zhang, Meifei Zhu, Shunwu Fan, Youqing Shen, Jiajia Xiang, Xin Liu   +10 more
wiley   +1 more source

The optimization of gene transfer to tomato and the study of expression possibility of salt-tolerance gene (SOS3)

یافته‌های نوین در علوم زیستی, 2020
One of the main strategies to improve plant tolerance is the expression of stress-induced genes, which play a significant role in the ionic balance of plants. SOS3 is one of the important components of SOS-regulated ionic homeostasis pathway.
Elham Mohajel Kazemi, Maghsoud Pazhohandeh, Parisa Jonoubi, Mina Kazemian   +3 more
doaj  

MRI of Transgene Expression: Correlation to Therapeutic Gene Expression

Neoplasia: An International Journal for Oncology Research, 2002
Magnetic resonance imaging (MRI) can provide highresolution 3D maps of structural and functional information, yet its use of mapping in vivo gene expression has only recently been explored.
Tomotsugu Ichikawa, Dagmar Högemann, Yoshinaga Saeki, Edyta Tyminski, Kinya Terada, Ralph Weissleder, E. Antonio Chiocca, James P. Basilion   +7 more
doaj   +1 more source

OTUD6A in Airway Epithelial Cells Exacerbates Allergic Asthma by Promoting Airway Inflammation and Airway Remodeling Through Deubiquitination of hResistin/mRELMα

Advanced Science, EarlyView.
This study elucidates a novel mechanistic role of the deubiquitinase OTUD6A in asthma pathogenesis, uncovering its regulatory function in airway inflammation and airway remodeling through the stabilization of hResistin/mRELMα. This study offers a novel regulatory axis (OTUD6A‐hResistin/mRELMα) in asthma pathogenesis and OTUD6A inhibition as a potential
Weiting Pan, Xinru Xi, Wei Dai, Tingfang Xiao, Yeqing Chen, Xuanyu Chen, Xiangting Ge, Chengguang Zhao, Hui Zhang, Yali Zhang, Weixi Zhang   +10 more
wiley   +1 more source