Results 181 to 190 of about 361,542 (380)

Chicken Pulmonary MicroRNAs Targeting the PB2 (Segment 1) of Avian Influenza Virus

Animal Research and One Health, EarlyView.
The PB2 segment of H5N1 is essential for replication and host adaptation. We screened 200 miRNAs and identified five (gga‐miR‐17‐3p, gga‐miR‐29a‐5p, gga‐miR‐1718, gga‐miR‐16c‐5p, and gga‐miR‐1744‐5p) using thermodynamic stability of heteroduplex, seed sequence complementarity, conservation, and accessibility, offering insights into host antiviral ...
Akanksha Choudhary, Nidhishree N. S, Rakesh Kumar Pundir, Muhasin Asaf V. N, Anamika Mishra, Ashwin Ashok Raut, Megha Agarwal, Amod Kumar   +7 more
wiley   +1 more source

Over‐Expression of a Phycocyanin‐Interferon Fusion and Differential Cleaving Efficiency in Cyanobacteria (Synechocystis sp. PCC 6803)

Biotechnology and Bioengineering, EarlyView.
ABSTRACT Recombinant protein expression in heterologous biological systems is an expanding field in synthetic biology. Photosynthetic organisms have the potential to provide an efficient low‐cost platform for recombinant protein production because they require minimal growth nutrients and are less susceptible to zoonotic and other contaminants ...
Bharat Kumar Majhi, Anastasios Melis
wiley   +1 more source

Adaptive Machine Learning Framework for Optimizing the Affinity Purification of Adeno‐Associated Viral Vectors

Biotechnology and Bioengineering, EarlyView.
ABSTRACT Adeno‐associated viral (AAV) vectors for gene therapy are becoming integral to modern medicine, providing therapeutic options for diseases once deemed incurable. Currently, viral vector purification is a critical bottleneck in the gene therapy industry, impacting product efficacy and safety as well as accessibility and cost to patients ...
Kelvin P. Idanwekhai, Shriarjun Shastry, Morgan R. Hurst, Arianna Minzoni, Eduardo Barbieri, Luke Remmler, Eugene N. Muratov, Michael A. Daniele, Stefano Menegatti, Alexander Tropsha   +9 more
wiley   +1 more source

The response of microbiome assembly within different niches across four stages to the cultivation of glyphosate-tolerant and conventional soybean varieties

Frontiers in Microbiology
IntroductionPlants are inherently connected with the microbiome, which plays a crucial role in regulating various host plant biological processes, including immunity, nutrient acquisition, and resistance against abiotic and biotic stresses.
Shengqian Chao, Shengqian Chao, Shengqian Chao, Yu Sun, Yu Sun, Yu Sun, Yin Zhang, Yin Zhang, Yin Zhang, Yifan Chen, Yifan Chen, Yifan Chen, Lili Song, Lili Song, Lili Song, Peng Li, Peng Li, Peng Li, Xueming Tang, Jingang Liang, Beibei Lv, Beibei Lv, Beibei Lv, Beibei Lv   +23 more
doaj   +1 more source

Efficient transfer, integration, and sustained long-term expression of the transgene in adult rat brains injected with a lentiviral vector.

Proceedings of the National Academy of Sciences of the United States of America, 1996
L. Naldini, U. Blömer, F. Gage, D. Trono, Inder M. Verma   +4 more
semanticscholar   +1 more source

Scale‐up of a monoclonal antibody CHO fed‐batch production in stirred tank bioreactors: Effect of hydrodynamic conditions and feeding regimen

Biotechnology Progress, EarlyView.
Abstract Key hydrodynamic‐related parameters such as volumetric power input (P/V), impeller configuration, aeration strategy, and maximum gas sparge rate, as well as an appropriate feeding strategy, must be carefully selected to improve production yields in bioreactor.
Lucas Lemire, Sebastian‐Juan Reyes, Yves Durocher, Robert Voyer, Olivier Henry, Phuong Lan Pham   +5 more
wiley   +1 more source

Escaping endogenous miRNA post-transcriptional silencing of JrGRF4b enhanced transformation efficiency in woody plants

Frontiers in Plant Science
The stable expression of transgenes was critically influenced by post-transcriptional regulatory mechanisms in transgenic plants. In this study, we investigated the influence of endogenous miRNA-mediated silencing on heterologous gene expression by ...
Baoxin Li, Mengting Pan, Dezheng Wu, Zhimin Zheng, Dong Pei   +4 more
doaj   +1 more source

Correction: Corrigendum: Rescue of neurophysiological phenotype seen in PRP null mice by transgene encoding human prion protein [PDF]

, 1995
Whittington, Miles A, Sidle, Katie CL, Gowland, Ian, Meads, Julie, Hill, Andrew F, Palmer, M, Jefferys, John GR, Collinge, John   +7 more
openalex   +1 more source

Enhancing CHO cell recombinant protein production using a perfusion‐directed host evolution approach

Biotechnology Progress, EarlyView.
Abstract Clonally derived cell lines generated from Chinese hamster ovary (CHO) cells encounter numerous stressors when cultured in high‐intensity perfusion bioreactors leading to poor process performance. To circumvent this, the ability of CHO cells to adapt to different culture environments was exploited. Here host cells were selected in the presence
Peter Amaya, Rajesh K. Mistry, Susie Sou, Sonja Hess, Bijay Khanal, Zayla Schaeffer, Jie Zhu, Lina Chakrabarti   +7 more
wiley   +1 more source

475. Functional Redundancy of Immunomodulatory Genes: Potent Protective Immunity Is Induced Against Pseudomonas Aeruginosa through Immunization with Antigen-Pulsed Dendritic Cells Genetically Modified with Different Immunomodulatory Transgenes [PDF]

, 2002

openalex   +1 more source