Results 11 to 20 of about 355,983 (382)

Heritable transgene-free genome editing in plants by grafting of wild-type shoots to transgenic donor rootstocks

Nature Biotechnology, 2023
Generation of stable gene-edited plant lines using clustered regularly interspaced short palindromic repeats (CRISPR)–CRISPR-associated protein 9 (Cas9) requires a lengthy process of outcrossing to eliminate CRISPR–Cas9-associated sequences and produce ...
Lei Yang, F. Machin, Shuangfeng Wang, E. Saplaoura, F. Kragler   +4 more
semanticscholar   +1 more source

Allele-aware chromosome-level genome assembly and efficient transgene-free genome editing for the autotetraploid cultivated alfalfa

Nature Communications, 2020
Artificially improving traits of cultivated alfalfa (Medicago sativa L.), one of the most important forage crops, is challenging due to the lack of a reference genome and an efficient genome editing protocol, which mainly result from its autotetraploidy ...
Haitao Chen, Yan Zeng, Yongzhi Yang, Lingli Huang, Bolin Tang, He Zhang, Fei Hao, Wei Liu, Youhan Li, Yanbin Liu, Xiaoshuang Zhang, Ru Zhang, Yesheng Zhang, Yongxin Li, Kun Wang, Hua He, Zhongkai Wang, Guangyi Fan, Hui Yang, Aike Bao, Z. Shang, Jianghua Chen, Wen Wang, Q. Qiu   +23 more
semanticscholar   +1 more source

Transgene Design [PDF]

, 2010
Transgenics are powerful mouse models to understand the biological functions of genes. This chapter gives a short overview of the requirements and considerations in designing a transgene. In addition, potential important choices that have to be made in advance for the successful designing and generating a transgenic mouse model are discussed.
van de Sluis, Bart, Voncken, Jan Willem
openaire   +3 more sources

Advances and potential pitfalls of oncolytic viruses expressing immunomodulatory transgene therapy for malignant gliomas

Cell Death and Disease, 2020
Glioblastoma (GBM) is an immunosuppressive, lethal brain tumor. Despite advances in molecular understanding and therapies, the clinical benefits have remained limited, and the life expectancy of patients with GBM has only been extended to ~15 months ...
Qing Zhang, Fusheng Liu
semanticscholar   +1 more source

Viral based vaccine TG4010 induces broadening of specific immune response and improves outcome in advanced NSCLC

Journal for ImmunoTherapy of Cancer, 2017
Background Advanced non-small cell lung cancer patients receiving TG4010, a therapeutic viral vaccine encoding human Mucin 1 and interleukin-2 in addition to standard chemotherapy, displayed longer overall survival in comparison to that of patients ...
Caroline Tosch, Bérangère Bastien, Luc Barraud, Benoit Grellier, Virginie Nourtier, Murielle Gantzer, Jean Marc Limacher, Eric Quemeneur, Kaïdre Bendjama, Xavier Préville   +9 more
doaj   +1 more source

Oncolytic virotherapy with intratumoral injection of vaccinia virus TG6002 and 5-fluorocytosine administration in dogs with malignant tumors

Molecular Therapy: Oncolytics, 2023
TG6002 is an oncolytic vaccinia virus expressing FCU1 protein, which converts 5-fluorocytosine into 5-fluorouracil. The study objectives were to assess tolerance, viral replication, 5-fluorouracil synthesis, and tumor microenvironment modifications to ...
Jérémy Béguin, Eve Laloy, Sandrine Cochin, Murielle Gantzer, Isabelle Farine, Christelle Pichon, Baptiste Moreau, Johann Foloppe, Jean-Marc Balloul, Christelle Machon, Jérôme Guitton, Dominique Tierny, Bernard Klonjkowski, Eric Quéméneur, Christelle Maurey, Philippe Erbs   +15 more
doaj   +1 more source

Immune-mediated loss of transgene expression from virally transduced brain cells is irreversible, mediated by IFNγ, perforin, and TNFα, and due to the elimination of transduced cells [PDF]

, 2012
The adaptive immune response to viral vectors reduces vector-mediated transgene expression from the brain. It is unknown, however, whether this loss is caused by functional downregulation of transgene expression or death of transduced cells.
Ahmed, Ali, Asadi-Moghaddam, Bantug, Barcia, Barcia, Barcia, Barcia, Barcia, Barcia, Bergmann, Byrnes, Byrnes, Carlos Barcia, Chen, Chiocca, Chunyan Liu, Conrady, Dewey, Diamond, Glass, Griffin, Griffin, Hausmann, Huse, Jeffrey M Zirger, Jiang, Jiang, Jiang, Josee Bergeron, Kanegae, Kurt M Kroeger, Lang, Lowenstein, Lowenstein, Maria G Castro, Mariana Puntel, McGavern, Mia Wibowo, Määttä, Niyati Bondale, O'Keefe, Parra, Paxinos, Pedro R Lowenstein, Pulkkanen, Puntel, Rall, Rameen Moridzadeh, Shi, Smith-Arica, Southgate, Southgate, Thomas, Thomas, Wang, Wilkinson, Williamson, Wirth, Yang, Zambrowicz   +60 more
core   +1 more source

A Myelin Proteolipid Protein-LacZ Fusion Protein Is Developmentally Regulated and Targeted to the Myelin Membrane in Transgenic Mice [PDF]

, 1993
Transgenic mice were generated with a fusion gene carrying a portion of the murine myelin proteolipid protein (PLP) gene, including the first intron, fused to the E. coli LacZ gene.
Duchala, Cynthia S., Macklin, Wendy B., Readhead, Carol, Wight, Patricia A.   +3 more
core   +2 more sources

Inactivation of the lysine binding sites of human plasminogen (hPg) reveals novel structural requirements for the tight hPg conformation, M-protein binding, and rapid activation

Frontiers in Molecular Biosciences, 2023
Accelerated activation of the human plasminogen zymogen (hPg) to two-chain active plasmin (hPm) is achieved following conformational changes induced by ligand-binding at the lysine-binding sites (LBSs) in four of the five hPg kringle domains.
Yetunde A. Ayinuola, Francis J. Castellino, Francis J. Castellino   +2 more
doaj   +1 more source

The changing role of cell culture in the generation of transgenic livestock [PDF]

, 1999
Transgenesis may allow the generation of farm animals with altered phenotype, animal models for research and animal bioreactors. Although such animals have been produced, the time and expense involved in generating transgenic livestock and then ...
Farini, E, Webster, J, Whitelaw, C B
core   +1 more source