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Transgene Design [PDF]

, 2010
Transgenics are powerful mouse models to understand the biological functions of genes. This chapter gives a short overview of the requirements and considerations in designing a transgene. In addition, potential important choices that have to be made in advance for the successful designing and generating a transgenic mouse model are discussed.
van de Sluis, Bart, Voncken, Jan Willem
openaire   +3 more sources

Advances and potential pitfalls of oncolytic viruses expressing immunomodulatory transgene therapy for malignant gliomas

Cell Death and Disease, 2020
Glioblastoma (GBM) is an immunosuppressive, lethal brain tumor. Despite advances in molecular understanding and therapies, the clinical benefits have remained limited, and the life expectancy of patients with GBM has only been extended to ~15 months ...
Qing Zhang, Fusheng Liu
semanticscholar   +1 more source

Viral based vaccine TG4010 induces broadening of specific immune response and improves outcome in advanced NSCLC

Journal for ImmunoTherapy of Cancer, 2017
Background Advanced non-small cell lung cancer patients receiving TG4010, a therapeutic viral vaccine encoding human Mucin 1 and interleukin-2 in addition to standard chemotherapy, displayed longer overall survival in comparison to that of patients ...
Caroline Tosch, Bérangère Bastien, Luc Barraud, Benoit Grellier, Virginie Nourtier, Murielle Gantzer, Jean Marc Limacher, Eric Quemeneur, Kaïdre Bendjama, Xavier Préville   +9 more
doaj   +1 more source

Inactivation of the lysine binding sites of human plasminogen (hPg) reveals novel structural requirements for the tight hPg conformation, M-protein binding, and rapid activation

Frontiers in Molecular Biosciences, 2023
Accelerated activation of the human plasminogen zymogen (hPg) to two-chain active plasmin (hPm) is achieved following conformational changes induced by ligand-binding at the lysine-binding sites (LBSs) in four of the five hPg kringle domains.
Yetunde A. Ayinuola, Francis J. Castellino, Francis J. Castellino   +2 more
doaj   +1 more source

Binding of the kringle‐2 domain of human plasminogen to streptococcal PAM‐type M‐protein causes dissociation of PAM dimers

MicrobiologyOpen, 2021
The direct binding of human plasminogen (hPg), via its kringle‐2 domain (K2hPg), to streptococcal M‐protein (PAM), largely contributes to the pathogenesis of Pattern D Group A Streptococcus pyogenes (GAS).
Olawole Ayinuola, Yetunde A. Ayinuola, Cunjia Qiu, Shaun W. Lee, Victoria A. Ploplis, Francis J. Castellino   +5 more
doaj   +1 more source

Plasminogen Deficiency Significantly Reduces Vascular Wall Disease in a Murine Model of Type IIa Hypercholesterolemia

Biomedicines, 2021
The fibrinolytic system has been implicated in the genesis and progression of atherosclerosis. It has been reported that a plasminogen (Pg) deficiency (Plg−/−) exacerbates the progression of atherosclerosis in Apoe−/− mice.
Takayuki Iwaki, Tomohiro Arakawa, Mayra J. Sandoval-Cooper, Denise L. Smith, Deborah Donahue, Victoria A. Ploplis, Kazuo Umemura, Francis J. Castellino   +7 more
doaj   +1 more source

Efficient and transgene-free genome editing in wheat through transient expression of CRISPR/Cas9 DNA or RNA

Nature Communications, 2016
Editing plant genomes is technically challenging in hard-to-transform plants and usually involves transgenic intermediates, which causes regulatory concerns.
Yi Zhang, Zhen Liang, Y. Zong, Yanpeng Wang, Jinxing Liu, Kunling Chen, J. Qiu, Caixia Gao   +7 more
semanticscholar   +1 more source

Pharmacokinetics and tolerance of repeated oral administration of 5-fluorocytosine in healthy dogs

BMC Veterinary Research, 2021
Background 5-fluorocytosine is a pyrimidine and a fluorinated cytosine analog mainly used as an antifungal agent. It is a precursor of 5-fluorouracil, which possesses anticancer properties.
Jérémy Béguin, Matthias Kohlhauer, Eve Laloy, Frédérique Degorce, Baptiste Moreau, Éric Quéméneur, Philippe Erbs, Bernard Klonjkowski, Christelle Maurey   +8 more
doaj   +1 more source

Relationships Between Plasminogen-Binding M-Protein and Surface Enolase for Human Plasminogen Acquisition and Activation in Streptococcus pyogenes

Frontiers in Microbiology, 2022
The proteolytic activity of human plasmin (hPm) is utilized by various cells to provide a surface protease that increases the potential of cells to migrate and disseminate. Skin-trophic Pattern D strains of Streptococcus pyogenes (GAS), e.g., GAS isolate
Yetunde A. Ayinuola, Sheiny Tjia-Fleck, Sheiny Tjia-Fleck, Bradley M. Readnour, Bradley M. Readnour, Zhong Liang, Olawole Ayinuola, Lake N. Paul, Shaun W. Lee, Vincent A. Fischetti, Victoria A. Ploplis, Victoria A. Ploplis, Francis J. Castellino, Francis J. Castellino   +13 more
doaj   +1 more source

Transgene-Free Genome Editing in Tomato and Potato Plants Using Agrobacterium-Mediated Delivery of a CRISPR/Cas9 Cytidine Base Editor

International Journal of Molecular Sciences, 2019
Genome editing tools have rapidly been adopted by plant scientists for gene function discovery and crop improvement. The current technical challenge is to efficiently induce precise and predictable targeted point mutations valuable for crop breeding ...
F. Veillet, Laura Perrot, L. Chauvin, M. Kermarrec, A. Guyon-Debast, J. Chauvin, F. Nogué, M. Mazier   +7 more
semanticscholar   +1 more source