Results 11 to 20 of about 361,542 (380)

Heritable transgene-free genome editing in plants by grafting of wild-type shoots to transgenic donor rootstocks

Nature Biotechnology, 2023
Generation of stable gene-edited plant lines using clustered regularly interspaced short palindromic repeats (CRISPR)–CRISPR-associated protein 9 (Cas9) requires a lengthy process of outcrossing to eliminate CRISPR–Cas9-associated sequences and produce ...
Lei Yang, F. Machin, Shuangfeng Wang, E. Saplaoura, F. Kragler   +4 more
semanticscholar   +1 more source

Design and selection of anti-PD-L1 single-domain antibody and tumor necrosis factor superfamily ligands for an optimal vectorization in an oncolytic virus

Frontiers in Bioengineering and Biotechnology, 2023
Arming oncolytic viruses with transgenes encoding immunomodulators improves their therapeutic efficacy by enhancing and/or sustaining the innate and adaptive anti-tumoral immune responses.
Christelle Remy, Elodie Pintado, Marshall Dunlop, Shirley Schön, Patricia Kleinpeter, Homa Rozanes, Laetitia Fend, Renée Brandely, Michel Geist, Delphine Suhner, Eline Winter, Nathalie Silvestre, Claire Huguet, Peter Fitzgerald, Eric Quéméneur, Jean-Baptiste Marchand   +15 more
doaj   +1 more source

Intravenous injection of a novel viral immunotherapy encoding human interleukin-7 in nonhuman primates is safe and increases absolute lymphocyte count

Human Vaccines & Immunotherapeutics, 2022
Persistence of an immunosuppression, affecting both the innate and adaptive arms of the immune system, plays a role in sepsis patients’ morbidity and late mortality pointing to the need for broad and effective immune interventions.
Charles-Antoine Coupet, Clarisse Dubois, Alexeï Evlachev, Nadine Kehrer, Marie Baldazza, Sam Hofman, Michel Vierboom, Perrine Martin, Geneviève Inchauspe   +8 more
doaj   +1 more source

Transgenic Overexpression of LARGE Induces alpha-Dystroglycan Hyperglycosylation in Skeletal and Cardiac Muscle [PDF]

, 2010
Background: LARGE is one of seven putative or demonstrated glycosyltransferase enzymes defective in a common group of muscular dystrophies with reduced glycosylation of alpha-dystroglycan.
Brockington, M, Brown, SC, Cirak, S, Liu, K, Muntoni, F, Sharp, PS, Torelli, S, Wells, DJ   +7 more
core   +14 more sources

The human ankyrin 1 promoter insulator sustains gene expression in a β-globin lentiviral vector in hematopoietic stem cells. [PDF]

, 2015
Lentiviral vectors designed for the treatment of the hemoglobinopathies require the inclusion of regulatory and strong enhancer elements to achieve sufficient expression of the β-globin transgene.
Baldwin, Kismet M, Campo-Fernandez, Beatriz, Cooper, Aaron R, Hoban, Megan D, Hollis, Roger P, Kaufman, Michael L, Kohn, Donald B, Lumaquin, Dianne, Romero, Zulema, Senadheera, Shantha, Urbinati, Fabrizia, Wang, Xiaoyan, Wherley, Jennifer   +12 more
core   +7 more sources

Allele-aware chromosome-level genome assembly and efficient transgene-free genome editing for the autotetraploid cultivated alfalfa

Nature Communications, 2020
Artificially improving traits of cultivated alfalfa (Medicago sativa L.), one of the most important forage crops, is challenging due to the lack of a reference genome and an efficient genome editing protocol, which mainly result from its autotetraploidy ...
Haitao Chen, Yan Zeng, Yongzhi Yang, Lingli Huang, Bolin Tang, He Zhang, Fei Hao, Wei Liu, Youhan Li, Yanbin Liu, Xiaoshuang Zhang, Ru Zhang, Yesheng Zhang, Yongxin Li, Kun Wang, Hua He, Zhongkai Wang, Guangyi Fan, Hui Yang, Aike Bao, Z. Shang, Jianghua Chen, Wen Wang, Q. Qiu   +23 more
semanticscholar   +1 more source

Safety, biodistribution and viral shedding of oncolytic vaccinia virus TG6002 administered intravenously in healthy beagle dogs

Scientific Reports, 2021
Oncolytic virotherapy is an emerging strategy that uses replication-competent viruses to kill tumor cells. We have reported the oncolytic effects of TG6002, a recombinant oncolytic vaccinia virus, in preclinical human xenograft models and canine tumor ...
Jérémy Béguin, Murielle Gantzer, Isabelle Farine, Johann Foloppe, Bernard Klonjkowski, Christelle Maurey, Éric Quéméneur, Philippe Erbs   +7 more
doaj   +1 more source

Vectorized Treg-depleting αCTLA-4 elicits antigen cross-presentation and CD8+ T cell immunity to reject ‘cold’ tumors

Journal for ImmunoTherapy of Cancer, 2022
Background Immune checkpoint blockade (ICB) is a clinically proven concept to treat cancer. Still, a majority of patients with cancer including those with poorly immune infiltrated ‘cold’ tumors are resistant to currently available ICB therapies ...
Laetitia Fend, Eric Quemeneur, Monika Semmrich, Jean-Baptiste Marchand, Matilda Rehn, Nathalie Silvestre, Linda Mårtensson, Johann Foloppe, Ingrid Teige, Björn Frendeus, Petra Holmkvist, Carolin Svensson, Christelle Remy, Patricia Kleinpeter, Jules Deforges, Fred Junghus, Kirstie L Cleary, Mimoza Bodén   +17 more
doaj   +1 more source

Transgene Design [PDF]

, 2010
Transgenics are powerful mouse models to understand the biological functions of genes. This chapter gives a short overview of the requirements and considerations in designing a transgene. In addition, potential important choices that have to be made in advance for the successful designing and generating a transgenic mouse model are discussed.
van de Sluis, Bart, Voncken, Jan Willem
openaire   +3 more sources

IL-7 producing immunotherapy improves ex vivo T cell functions of immunosenescent patients, especially post hip fracture

Human Vaccines & Immunotherapeutics, 2023
Following acute stress such as trauma or sepsis, most of critically ill elderly patients become immunosuppressed and susceptible to secondary infections and enhanced mortality.
Chrystel Marton, Alix Minaud, Charles-Antoine Coupet, Manon Chauvin, Jamila Dhiab, Hélène Vallet, Jacques Boddaert, Nadine Kehrer, Bérangère Bastien, Geneviève Inchauspe, Luc Barraud, Delphine Sauce   +11 more
doaj   +1 more source