Results 281 to 290 of about 293,415 (335)

A chimeric viral platform for directed evolution in mammalian cells. [PDF]

Nat Commun
Cole AJ, Denes CE, Moreno CL, Hunault L, Dobson T, Hesselson D, Neely GG.   +6 more
europepmc   +1 more source

A deletion upstream of the degron of AcIAA27 leads to auxinic herbicide resistance in Arctotheca calendula

Pest Management Science, EarlyView.
An in‐frame deletion was identified in AcIAA27, compared to the susceptible population, which confer resistance to both 2,4‐D and dicamba. Abstract BACKGROUND Two populations of Arctotheca calendula (capeweed) from near Naracoorte, South Australia, were not controlled by 2,4‐D. These populations were investigated for resistance to 2,4‐D and other auxin
Yuanlin Qi, Mahima Krishnan, Matthew Tucker, Peter Boutsalis, Christopher Preston   +4 more
wiley   +1 more source

Nature Inspired Delivery Vehicles for CRISPR‐Based Genome Editing

Small, EarlyView.
The review highlights nature‐inspired nanocarriers for CRISPR delivery, emphasizing viral vectors, extracellular vesicles, liposomes, and lipid nanoparticles. It discusses their roles in improving specificity, minimizing immunogenicity, and overcoming barriers in genome editing. Recent advancements, challenges, and therapeutic applications are explored,
Elizabeth Maria Clarissa, Mamata Karmacharya, Hyunmin Choi, Sumit Kumar, Yoon‐Kyoung Cho   +4 more
wiley   +1 more source

AAV yield, bioactivity, and particle heterogeneity are impacted by genome size and non-coding DNA elements. [PDF]

Mol Ther Methods Clin Dev
Blahetek G, Lindner B, Oti M, Schön C, Strobel B.   +4 more
europepmc   +1 more source

3D Quantification of Viral Transduction Efficiency in Living Human Retinal Organoids

Small Methods, EarlyView.
Optimization of viral vectors and their testing in human tissue is crucial to gene therapy development. Conventional technologies to assess virus transduction either lack spatial or temporal information. Here, spatiotemporal mapping of virus transduction in living retinal tissue is introduced, levering fluorescence imaging and machine learning‐based ...
Teresa S. Rogler, Katja A. Salbaum, Achim T. Brinkop, Selina M. Sonntag, Rebecca James, Elijah R. Shelton, Alina Thielen, Roland Rose, Sabrina Babutzka, Thomas Klopstock, Stylianos Michalakis, Friedhelm Serwane   +11 more
wiley   +1 more source

Delivery of a Muscle-Targeted Adeno-Associated Vector Via <i>Ex Vivo</i> Normothermic Perfusion Is Efficient, Durable, and Safe in a Preclinical Porcine Heart Transplant Model. [PDF]

Transpl Int
Dewan KC, Chen JW, Lobo AA, Gross RT, Wang C, Rivera KG, Tran KD, Ngeve S, Johnston VG, Wendell D, Glass CK, Evans A, Ho S, Lezberg P, Casy W, Bazile M, Patel K, Cockrell AS, Milano CA, Bowles D.   +19 more
europepmc   +1 more source

Manipulation of Wnt/β‐Catenin Signaling by Synthetic Frizzled Agonist and LRP Antagonist in Organoid Cultures and In Vivo

Small Methods, EarlyView.
Harnessing synthetic key receptor modulators, this study presents a breakthrough in Wnt/β‐catenin regulation. The FZD agonist RRP‐pbFn bypasses LRP5/6 to drive organoid expansion and tissue regeneration, while the LRP antagonist RRP‐Dkk1c eradicates tumors. These tools enable precise pathway control, advancing regenerative and cancer therapies.
Quanhui Dai, Jiawen Wang, Zihuan Lin, Danni Yu, Hui Yang, Jinsong Wei, Xiaoyu Li, Hao Hu, Chao Ni, Bing Zhao   +9 more
wiley   +1 more source

Enhancing the potency of in vivo lentiviral vector mediated gene therapy to hepatocytes. [PDF]

Nat Commun
Canepari C, Milani M, Simoni C, Starinieri F, Volpin M, Fabiano A, Biffi M, Russo F, Norata R, Rocchi M, Brombin C, Cugnata F, Montini E, Sanvito F, Grompe M, Cantore A.   +15 more
europepmc   +1 more source

CRISPR/Cas9‐edited tumor‐associated immune cells in cancer immunotherapy

VIEW, EarlyView.
Abstract Immuno‐oncology represents an emerging field that has significantly transformed tumor therapeutics, with immune cells serving as the cellular foundations of cancer immunotherapy. Due to its high efficiency and sensitivity, CRISPR/Cas9 genome editing is a highly promising technique for precise and rapid gene modification.
Yuhui Ma, Junxin Chen, Yuan Du, Zhihao Cao, Weizhi Ji, Yuyu Niu, Zebin Yang   +6 more
wiley   +1 more source