Results 21 to 30 of about 227,142 (341)

Fluorescent Tagged Vaccinia Virus Genome Allows Rapid and Efficient Measurement of Oncolytic Potential and Discovery of Oncolytic Modulators

Biomedicines, 2020
As a live biologic agent, oncolytic vaccinia virus has the ability to target and selectively amplify at tumor sites. We have previously reported that deletion of thymidine kinase and ribonucleotide reductase genes in vaccinia virus can increase the ...
Franck Gallardo, Doris Schmitt, Renée Brandely, Catherine Brua, Nathalie Silvestre, Annie Findeli, Johann Foloppe, Sokunthea Top, Sandrine Kappler-Gratias, Charlotte Quentin-Froignant, Renaud Morin, Jean-Michel Lagarde, Kerstin Bystricky, Stéphane Bertagnoli, Philippe Erbs   +14 more
doaj   +1 more source

Heterologous Production and Evaluation of the Biological Activity of Cystatin-B From the Red Piranha Pygocentrus nattereri

Frontiers in Genetics, 2022
Cystatin proteins are known to form a superfamily of cysteine protease inhibitors, which play a key role in protein degradation and are related to different physiological processes, such as development and immunity.
Juan Antonio Ramirez Merlano, Daniela Volcan Almeida   +1 more
doaj   +1 more source

Rapid generation of a transgene-free powdery mildew resistant tomato by genome deletion

Scientific Reports, 2017
Genome editing has emerged as a technology with a potential to revolutionize plant breeding. In this study, we report on generating, in less than ten months, Tomelo, a non-transgenic tomato variety resistant to the powdery mildew fungal pathogen using ...
V. Nekrasov, Congmao Wang, J. Win, C. Lanz, D. Weigel, S. Kamoun   +5 more
semanticscholar   +1 more source

Sequential administration of MVA-based vaccines and PD-1/PD-L1-blocking antibodies confers measurable benefits on tumor growth and survival: Preclinical studies with MVA-βGal and MVA-MUC1 (TG4010) in a murine tumor model

Human Vaccines & Immunotherapeutics, 2018
TG4010, a Modified Vaccinia virus Ankara (MVA) expressing human mucin1 (MUC1) has demonstrated clinical benefit for patients suffering from advanced non-small cell lung cancer (NSCLC) in combination with chemotherapy.
Christelle Remy-Ziller, Christine Thioudellet, Julie Hortelano, Murielle Gantzer, Virginie Nourtier, Marie-Christine Claudepierre, Benoit Sansas, Xavier Préville, Kaïdre Bendjama, Eric Quemeneur, Karola Rittner   +10 more
doaj   +1 more source

Cloning, expression and functional analysis of the SOC1 homologous gene in pak choi (Brassica rapa ssp. Chinensis makino)

Biotechnology & Biotechnological Equipment, 2022
Flowering transition is important in plant growth and development, and the SUPPRESSOR OF OVEREXPRESSION OF CO 1 (SOC1) gene belongs to the MADS-box gene family, which is an integrator involved in the regulation of flower formation in plants.
Xuan Li, Amin Ping, Xianhui Qi, Meilan Li, Leiping Hou   +4 more
doaj   +1 more source

CtIP fusion to Cas9 enhances transgene integration by homology-dependent repair

Nature Communications, 2018
In genome editing with CRISPR–Cas9, transgene integration often remains challenging. Here, we present an approach for increasing the efficiency of transgene integration by homology-dependent repair (HDR).
M. Charpentier, A. Khedher, S. Ménoret, A. Brion, K. Lamribet, E. Dardillac, C. Boix, L. Perrouault, L. Tesson, S. Geny, A. D. Cian, J. Itier, I. Anegon, B. Lopez, C. Giovannangeli, J. Concordet   +15 more
semanticscholar   +1 more source

Engineering of CRISPR/Cas9‐mediated potyvirus resistance in transgene‐free Arabidopsis plants

Molecular plant pathology, 2016
Summary Members of the eukaryotic translation initiation factor (eIF) gene family, including eIF4E and its paralogue eIF(iso)4E, have previously been identified as recessive resistance alleles against various potyviruses in a range of different hosts ...
D. E. Pyott, Emma Sheehan, A. Molnár
semanticscholar   +1 more source

Transgene-design: a web application for the design of mammalian transgenes [PDF]

Bioinformatics, 2022
AbstractSummaryTransgene-design is a web application to help design transgenes for use in mammalian studies. It is predicated on the recent discovery that human intronless transgenes and native retrogenes can be expressed very effectively if the GC content at exonic synonymous sites is high.
Stefanie Mühlhausen, Laurence D Hurst
openaire   +2 more sources

Efficient transfer, integration, and sustained long-term expression of the transgene in adult rat brains injected with a lentiviral vector.

Proceedings of the National Academy of Sciences of the United States of America, 1996
We describe the construction of a safe, replication-defective and efficient lentiviral vector suitable for in vivo gene delivery. The reverse transcription of the vector was found to be a rate-limiting step; therefore, promoting the reaction inside the ...
L. Naldini, U. Blömer, F. Gage, D. Trono, Inder M. Verma   +4 more
semanticscholar   +1 more source

A Potential Method for Rapid Screening of Amphioxus Founder Harboring Germline Mutation and Transgene

Frontiers in Cell and Developmental Biology, 2021
Amphioxus is a promising model organism for understanding the origin and evolution of vertebrates due to its basal phylogenetic position among chordates.
Jiaqi Zou, Xiaotong Wu, Chenggang Shi, Yanhong Zhong, Lei Zhang, Qiuning Yan, Liuru Su, Guang Li   +7 more
doaj   +1 more source