Results 21 to 30 of about 355,983 (382)

Efficient and transgene-free genome editing in wheat through transient expression of CRISPR/Cas9 DNA or RNA

Nature Communications, 2016
Editing plant genomes is technically challenging in hard-to-transform plants and usually involves transgenic intermediates, which causes regulatory concerns.
Yi Zhang, Zhen Liang, Y. Zong, Yanpeng Wang, Jinxing Liu, Kunling Chen, J. Qiu, Caixia Gao   +7 more
semanticscholar   +1 more source

Fluorescent Tagged Vaccinia Virus Genome Allows Rapid and Efficient Measurement of Oncolytic Potential and Discovery of Oncolytic Modulators

Biomedicines, 2020
As a live biologic agent, oncolytic vaccinia virus has the ability to target and selectively amplify at tumor sites. We have previously reported that deletion of thymidine kinase and ribonucleotide reductase genes in vaccinia virus can increase the ...
Franck Gallardo, Doris Schmitt, Renée Brandely, Catherine Brua, Nathalie Silvestre, Annie Findeli, Johann Foloppe, Sokunthea Top, Sandrine Kappler-Gratias, Charlotte Quentin-Froignant, Renaud Morin, Jean-Michel Lagarde, Kerstin Bystricky, Stéphane Bertagnoli, Philippe Erbs   +14 more
doaj   +1 more source

Binding of the kringle‐2 domain of human plasminogen to streptococcal PAM‐type M‐protein causes dissociation of PAM dimers

MicrobiologyOpen, 2021
The direct binding of human plasminogen (hPg), via its kringle‐2 domain (K2hPg), to streptococcal M‐protein (PAM), largely contributes to the pathogenesis of Pattern D Group A Streptococcus pyogenes (GAS).
Olawole Ayinuola, Yetunde A. Ayinuola, Cunjia Qiu, Shaun W. Lee, Victoria A. Ploplis, Francis J. Castellino   +5 more
doaj   +1 more source

Plasminogen Deficiency Significantly Reduces Vascular Wall Disease in a Murine Model of Type IIa Hypercholesterolemia

Biomedicines, 2021
The fibrinolytic system has been implicated in the genesis and progression of atherosclerosis. It has been reported that a plasminogen (Pg) deficiency (Plg−/−) exacerbates the progression of atherosclerosis in Apoe−/− mice.
Takayuki Iwaki, Tomohiro Arakawa, Mayra J. Sandoval-Cooper, Denise L. Smith, Deborah Donahue, Victoria A. Ploplis, Kazuo Umemura, Francis J. Castellino   +7 more
doaj   +1 more source

Transgene-Free Genome Editing in Tomato and Potato Plants Using Agrobacterium-Mediated Delivery of a CRISPR/Cas9 Cytidine Base Editor

International Journal of Molecular Sciences, 2019
Genome editing tools have rapidly been adopted by plant scientists for gene function discovery and crop improvement. The current technical challenge is to efficiently induce precise and predictable targeted point mutations valuable for crop breeding ...
F. Veillet, Laura Perrot, L. Chauvin, M. Kermarrec, A. Guyon-Debast, J. Chauvin, F. Nogué, M. Mazier   +7 more
semanticscholar   +1 more source

Relationships Between Plasminogen-Binding M-Protein and Surface Enolase for Human Plasminogen Acquisition and Activation in Streptococcus pyogenes

Frontiers in Microbiology, 2022
The proteolytic activity of human plasmin (hPm) is utilized by various cells to provide a surface protease that increases the potential of cells to migrate and disseminate. Skin-trophic Pattern D strains of Streptococcus pyogenes (GAS), e.g., GAS isolate
Yetunde A. Ayinuola, Sheiny Tjia-Fleck, Sheiny Tjia-Fleck, Bradley M. Readnour, Bradley M. Readnour, Zhong Liang, Olawole Ayinuola, Lake N. Paul, Shaun W. Lee, Vincent A. Fischetti, Victoria A. Ploplis, Victoria A. Ploplis, Francis J. Castellino, Francis J. Castellino   +13 more
doaj   +1 more source

Sustained gene expression in the retina by improved episomal vectors [PDF]

, 2014
Gene and cellular therapies are nowadays part of therapeutic strategies for the treatment of diverse pathologies. The drawbacks associated with gene therapy-low levels of transgene expression, vector loss during mitosis, and gene silencing-need to be ...
Ana V. Oliveira, Conley S.M., Davis A.A., Gabriela A. Silva, Reinisalo M., Rudolf Haase, Sofia M. Calado, Susana Machado   +7 more
core   +1 more source

Rapid generation of a transgene-free powdery mildew resistant tomato by genome deletion

Scientific Reports, 2017
Genome editing has emerged as a technology with a potential to revolutionize plant breeding. In this study, we report on generating, in less than ten months, Tomelo, a non-transgenic tomato variety resistant to the powdery mildew fungal pathogen using ...
V. Nekrasov, Congmao Wang, J. Win, C. Lanz, D. Weigel, S. Kamoun   +5 more
semanticscholar   +1 more source

Sequential administration of MVA-based vaccines and PD-1/PD-L1-blocking antibodies confers measurable benefits on tumor growth and survival: Preclinical studies with MVA-βGal and MVA-MUC1 (TG4010) in a murine tumor model

Human Vaccines & Immunotherapeutics, 2018
TG4010, a Modified Vaccinia virus Ankara (MVA) expressing human mucin1 (MUC1) has demonstrated clinical benefit for patients suffering from advanced non-small cell lung cancer (NSCLC) in combination with chemotherapy.
Christelle Remy-Ziller, Christine Thioudellet, Julie Hortelano, Murielle Gantzer, Virginie Nourtier, Marie-Christine Claudepierre, Benoit Sansas, Xavier Préville, Kaïdre Bendjama, Eric Quemeneur, Karola Rittner   +10 more
doaj   +1 more source

The Enhanced Tumor Specificity of TG6002, an Armed Oncolytic Vaccinia Virus Deleted in Two Genes Involved in Nucleotide Metabolism

Molecular Therapy: Oncolytics, 2019
Oncolytic vaccinia viruses are currently in clinical development. However, the safety and the tumor selectivity of these oncolytic viruses must be improved.
Johann Foloppe, Juliette Kempf, Nicolas Futin, Jacqueline Kintz, Pascale Cordier, Christelle Pichon, Annie Findeli, Fabien Vorburger, Eric Quemeneur, Philippe Erbs   +9 more
doaj   +1 more source