Results 291 to 300 of about 361,542 (380)

Functional validation of a white pupae minimal gene construct in Ceratitis capitata (Diptera: Tephritidae)

Insect Science, EarlyView.
An intronless version of the white pupae (wp) gene was engineered to restore the wild type brown puparium color in white pupae phenotype mutants of the Mediterranean fruit fly, Ceratitis capitata. Functionality of the minimal gene version (mini‐wp) was verified in vivo, as one copy of mini‐wp successfully restored the wild type phenotype in homozygous ...
Lucas Henrique Figueiredo Prates, Roswitha A. Aumann, Inga Sievers, Tanja Rehling, Marc F. Schetelig   +4 more
wiley   +1 more source

Double-strand break-free and transgene-free genome editing in the microalga Nannochloropsis oceanica using removable vectors containing the CRISPR base editing system. [PDF]

Sci Rep
Moroi K, Yamamoto T, Kurita T.
europepmc   +1 more source

Long-term transgene expression in mouse neural progenitor cells modified with ϕC31 integrase

, 2008
Annahita Keravala, Brandi K. Ormerod, Theo D. Palmer, Michèle P. Calos   +3 more
openalex   +1 more source

Maltase 1 regulates DENV2 infection and life history in Aedes aegypti

Insect Science, EarlyView.
Our study employed CRISPR/Cas9 to generate a MAL1 knockout strain of Aedes aegypti, demonstrating that the absence of MAL1 significantly suppresses DENV2 replication in the midgut and impairs viral transmission. The mutation also altered life‐history (hatching/pupation/emerging rates and sex‐specific survival), identifying MAL1 as a novel target for ...
Man‐Jin Li, Jin Cheng, Ya‐Ming Zou, Quan‐Chao Liu, Ding Zhu, Ce‐Jie Lan   +5 more
wiley   +1 more source

Highly efficient transgenesis mediated by Tip100 transposon system in medaka. [PDF]

Transgenic Res
Tanaka Y, Seki T, Hoshino A, Ansai S.
europepmc   +1 more source

Sequence and expression analysis of potential spermatogenesis‐specific gene cognates in the Caribbean fruit fly, Anastrepha suspensa

Insect Science, EarlyView.
Abstract The sterile insect technique (SIT) is a highly effective biologically‐based method for the suppression of many insect pest populations. SIT efficacy could be improved by methods of male sterilization that avoid the use of irradiation that can result in diminished fitness and mating competitiveness.
Alfred M. Handler, Richard B. Furlong, Chao Chen, Daniel A. Hahn   +3 more
wiley   +1 more source

Reversing transgene silencing via targeted chromatin editing [PDF]

Sebastian Palacios, Elia Salibi, Eric Lu, Ron Weiss, Thorsten M. Schlaeger, James J. Collins, Domitilla Del Vecchio   +6 more
openalex   +1 more source

DsiRNA‐mediated silencing of Ceratitis capitata transformer or transformer‐2 leads to masculinization of XX embryos and systemic gene silencing in ovaries

Insect Science, EarlyView.
We carried out functional studies using Dicer‐substrate small interfering RNAs (DsiRNAs), targeting the sex determination genes Cctransformer (Cctra) and Cctransformer‐2 (Cctra‐2) in the Mediterranean fruit fly (Ceratitis capitata). Microinjection of DsiRNAs into XX embryos (0‐1 h old) rapidly triggered the expression of male‐specific Cctra isoforms ...
Gennaro Volpe, Sarah Maria Mazzucchiello, Domenico De Falco, David Torrente, Stefania Liguori, Noemi Rosati, Dora Baccaro, Michela Mazzeo, Fulvio Bertolotto, Harshini Sangle, Ennio Giordano, Angela Carfora, Francesca Lucibelli, Marianna Varone, Paola Di Lillo, Hugo Diego Perdomo, Mariangela Bonizzoni, Serena Aceto, Marco Salvemini, Molly Duman‐Scheel, Giuseppe Saccone   +20 more
wiley   +1 more source

Aberrant RNA identification reveals triggers of transgene silencing. [PDF]

Plant Cell
Willoughby AC.
europepmc   +1 more source

Imaging Transgene Expression with Radionuclide Imaging Technologies

, 2000
Sanjiv S. Gambhir, Harvey R. Herschman, Simon R. Cherry, Jorge R. Barrio, Nagichettiar Satyamurthy, T. TOYOKUNI, Michael E. Phelps, Steven M. Larson, Julius Balatoni, R. Finn, Michel Sadelain, Juri G. Gelovani, R. Blasbergt   +12 more
openalex   +1 more source