Results 41 to 50 of about 227,142 (341)

Transgene Expression and Bt Protein Content in Transgenic Bt Maize (MON810) under Optimal and Stressful Environmental Conditions

PLoS ONE, 2015
Bt protein content in transgenic insect resistant (Bt) maize may vary between tissues within plants and between plants growing under different environmental conditions.
Miluse Trtikova, O. Wikmark, N. Zemp, A. Widmer, A. Hilbeck   +4 more
semanticscholar   +1 more source

Four Core Genotypes mouse model: localization of the Sry transgene and bioassay for testicular hormone levels

BMC Research Notes, 2015
The “four core genotypes” (FCG) mouse model has emerged as a major model testing if sex differences in phenotypes are caused by sex chromosome complement (XX vs. XY) or gonadal hormones or both.
Y. Itoh, Ryan Mackie, K. Kampf, Shelly Domadia, Judith D. Brown, R. O’Neill, A. Arnold   +6 more
semanticscholar   +1 more source

Transgenic livestock

Bioscientifica Proceedings, 2019
Single genes can now be added routinely to the genome of mice by molecular manipulation as simple Mendelian dominants; this complements the normal process of reproduction to give 'transgenic' animals. Success in ruminants is limited to a few examples in sheep and although gene expression has yet to be documented, there is every reason to expect that it
J P, Simons, R B, Land
openaire   +2 more sources

Insights into pegRNA design from editing of the cardiomyopathy‐associated phospholamban R14del mutation

FEBS Letters, EarlyView.
This study reveals how prime editing guide RNA (pegRNA) secondary structure and reverse transcriptase template length affect prime editing efficiency in correcting the phospholamban R14del cardiomyopathy‐associated mutation. Insights support the design of structurally optimized enhanced pegRNAs for precise gene therapy.
Bing Yao, Qiangbing Yang, Christian J.B. Snijders Blok, Mark A. Daniels, Pieter A. Doevendans, Raymond Schiffelers, Joost P.G. Sluijter, Zhiyong Lei   +7 more
wiley   +1 more source

Plasminogen Activator Inhibitor-1 Protects Mice Against Cardiac Fibrosis by Inhibiting Urokinase-type Plasminogen Activator-mediated Plasminogen Activation

Scientific Reports, 2017
Plasminogen activator inhibitor-1 (PAI-1) is known to protect mice against cardiac fibrosis. It has been speculated that PAI-1 may regulate cardiac fibrosis by inactivating urokinase-type plasminogen activator (uPA) and ultimately plasmin (Pm) generation.
Kamlesh K. Gupta, Deborah L. Donahue, Mayra J. Sandoval-Cooper, Francis J. Castellino, Victoria A. Ploplis   +4 more
doaj   +1 more source

Stability of the HSV-2 US-6 Gene in the del II, del III, CP77, and I8R-G1L Sites in Modified Vaccinia Virus Ankara After Serial Passage of Recombinant Vectors in Cells

Vaccines, 2020
The modified vaccinia virus Ankara (MVA), a severely attenuated strain of vaccinia virus, is a promising vector platform for viral-vectored vaccine development because of its attributes of efficient transgene expression and safety profile, among others ...
Vajini N. Atukorale, Jerry P. Weir, Clement A. Meseda   +2 more
doaj   +1 more source

Long-Term Efficacy and Safety of RNAi-Mediated Virus Resistance in ‘HoneySweet’ Plum

Frontiers in Plant Science, 2021
Interfering RNA technology has been established as an effective strategy to protect plants against viral infection. Despite this success, interfering RNA (RNAi) has rarely been applied due to the regulatory barriers that confront genetically engineered ...
Khushwant Singh, Khushwant Singh, Ann M. Callahan, Brenda J. Smith, Tadeusz Malinowski, Tadeusz Malinowski, Ralph Scorza, Jana Jarošová, Eva Beoni, Jaroslav Polák, Jiban Kumar Kundu, Chris Dardick   +11 more
doaj   +1 more source

Determination of ADP/ATP translocase isoform ratios in malignancy and cellular senescence

Molecular Oncology, EarlyView.
The individual functions of three isoforms exchanging ADP and ATP (ADP/ATP translocases; ANTs) on the mitochondrial membrane remain unclear. We developed a method for quantitatively differentiating highly similar human ANT1, ANT2, and ANT3 using parallel reaction monitoring. This method allowed us to assess changes in translocase levels during cellular
Zuzana Liblova, Dominika Maurencova, Barbora Salovska, Marek Kratky, Tomas Mracek, Zuzana Korandova, Alena Pecinova, Pavla Vasicova, David Rysanek, Ladislav Andera, Ivo Fabrik, Rudolf Kupcik, Pavel Kashmel, Pinky Sultana, Vojtech Tambor, Jiri Bartek, Josef Novak, Marie Vajrychova, Zdenek Hodny   +18 more
wiley   +1 more source

Random and targeted transgene insertion in C. elegans using a modified Mosl transposon

Nature Methods, 2014
We have generated a recombinant Mos1 transposon that can insert up to 45-kb transgenes into the Caenorhabditis elegans genome. The minimal Mos1 transposon (miniMos) is 550 bp long and inserts DNA into the genome at high frequency (∼60% of injected ...
Christian Frøkjær-Jensen, M. Davis, M. Sarov, Jon Taylor, S. Flibotte, Matthew L Labella, Andrei Pozniakovsky, D. Moerman, Erik M. Jorgensen   +8 more
semanticscholar   +1 more source

Neuroanatomy and transgenic technologies [PDF]

Frontiers in Neuroanatomy, 2015
Neuroanatomists increasingly rely on techniques enabling them to manipulate genes in defined brain cell populations. In particular, engineered transgenes, which encode a variety of fluorescent reporter proteins can be inserted into the genome or delivered into desired brain regions using viral vectors, thereby allowing the labeling of molecularly ...
Laurent Gautron, Alexander C. Jackson, Chen Liu, Makoto Fukuda, Michael Lazarus   +4 more
openaire   +5 more sources