Results 81 to 90 of about 227,142 (341)

Assessment of Fatty Acid Desaturase (Fads2) Structure-Function Properties in Fish in the Context of Environmental Adaptations and as a Target for Genetic Engineering

Biomolecules, 2020
Fatty acid desaturase 2 (Fads2) is the key enzyme of long-chain polyunsaturated fatty acid (LC-PUFA) biosynthesis. Endogenous production of these biomolecules in vertebrates, if present, is insufficient to meet demand.
Zuzana Bláhová, Thomas Nelson Harvey, Martin Pšenička, Jan Mráz   +3 more
doaj   +1 more source

AAVR Expression is Essential for AAV Vector Transduction in Sensory Hair Cells

Advanced Science, EarlyView.
Decreased sensitivity to AAV vector transduction in the outer hair cells (OHCs) of adult mice is primarily attributed to reduction of AAVR (Kiaa0319l; Au040320). Knockout of AAVR reduces AAV vector transduction efficiency in both inner hair cells (IHCs) and OHCs in neonatal mice.
Fan Wu, Guisheng Chen, Rui Hu, Peiwen Liu, Jintao Lou, Wenji Zhao, Zuhong He, Suhua Sha, Yiqing Zheng   +8 more
wiley   +1 more source

Single Administration of AAV‐mAtp6v1b2 Gene Therapy Rescues Hearing and Vestibular Disorders Caused by Atp6v1b2‐Induced Lysosomal Dysfunction in Hair Cells

Advanced Science, EarlyView.
Wei et al. establish a hair cell‐specific conditional knockout mouse model (Atp6v1b2fl/fl;Atoh1Cre/+), and demonstrate the importance of Atp6v1b2 for hair cell through maintaining the survival of lysosomes. A single administration of AAV‐ie‐Eh3‐mAtp6v1b2 through scala media at P0‐P2 realizes function compensation and restores hearing and balance ...
Gege Wei, Shiwei Qiu, Xue Gao, Lu Zheng, Yijin Chen, Ying Ma, Haifeng Feng, Jinyuan Yang, Guojie Dong, Huiyi Nie, Weihao Zhao, Xiaoge Li, Guangqin Wang, Wei Xiong, Pu Dai, Yongyi Yuan   +15 more
wiley   +1 more source

Preclinical Evaluation of the Oncolytic Vaccinia Virus TG6002 by Translational Research on Canine Breast Cancer

Molecular Therapy: Oncolytics, 2020
Oncolytic virotherapy is a promising therapeutic approach for the treatment of cancer. TG6002 is a recombinant oncolytic vaccinia virus deleted in the thymidine kinase and ribonucleotide reductase genes and armed with the suicide gene FCU1, which encodes
Jérémy Béguin, Johann Foloppe, Christelle Maurey, Eve Laloy, Julie Hortelano, Virginie Nourtier, Christelle Pichon, Sandrine Cochin, Pascale Cordier, Hélène Huet, Eric Quemeneur, Bernard Klonjkowski, Philippe Erbs   +12 more
doaj  

AAV capsid variants with brain-wide transgene expression and decreased liver targeting after intravenous delivery in mouse and marmoset

Nature Neuroscience, 2021
David Goertsen, Nicholas C. Flytzanis, N. Goeden, Miguel R. Chuapoco, A. Cummins, Yijing Chen, Yingying Fan, Qiangge Zhang, Jitendra Sharma, Yangyang Duan, Liping Wang, G. Feng, Yu Chen, N. Ip, J. Pickel, V. Gradinaru   +15 more
semanticscholar   +1 more source

Transgene expression up to 7 years in nonhuman primates following hepatic transduction with helper-dependent adenoviral vectors.

Human Gene Therapy, 2013
Helper-dependent adenoviral vectors (HDAd) have been shown to mediate a considerably longer duration of transgene expression than first-generation adenoviral vectors. We have previously shown that transgene expression from HDAd-transduced hepatocytes can
N. Brunetti‐Pierri, T. Ng, D. Iannitti, W. Cioffi, Gary E. Stapleton, Mark Law, J. Breinholt, D. Palmer, Nathan C Grove, K. Rice, Cassondra Bauer, M. Finegold, A. Beaudet, C. Mullins, P. Ng   +14 more
semanticscholar   +1 more source

The Morphological, Behavioral, and Transcriptomic Life Cycle of Anthrobots

Advanced Science, EarlyView.
DNA is thought to determine shape, behavior, and lifespan, with developmental plasticity reserved for stem cells, embryos, and species like amphibia. How much morphogenetic plasticity do adult somatic human cells exhibit? Anthrobots are a self‐organizing, motile synthetic living construct made of genetically normal human epithelial cells.
Gizem Gumuskaya, Nikolai Davey, Pranjal Srivastava, Andrew Bender, Léo Pio‐Lopez, Douglas Hazel, Michael Levin   +6 more
wiley   +1 more source

Cowpox Virus: A New and Armed Oncolytic Poxvirus

Molecular Therapy: Oncolytics, 2017
Oncolytic virus therapy has recently been recognized as a promising new therapeutic approach for cancer treatment. In this study, we are proposing for the first time to evaluate the in vitro and in vivo oncolytic capacities of the Cowpox virus (CPXV). To
Marine Ricordel, Johann Foloppe, Christelle Pichon, Nathalie Sfrontato, Delphine Antoine, Caroline Tosch, Sandrine Cochin, Pascale Cordier, Eric Quemeneur, Christelle Camus-Bouclainville, Stéphane Bertagnoli, Philippe Erbs   +11 more
doaj  

Global Screening of Antiviral Genes that Suppress Baculovirus Transgene Expression in Mammalian Cells

Molecular Therapy: Methods & Clinical Development, 2017
Although baculovirus has been used as a safe and convenient gene delivery vector in mammalian cells, baculovirus-mediated transgene expression is less effective in various mammalian cell lines.
Chia-Hung Wang, Nenavath Gopal Naik, Lin-Li Liao, Sung-Chan Wei, Yu-Chan Chao   +4 more
doaj   +1 more source

A mini-intronic plasmid (MIP): a novel robust transgene expression vector in vivo and in vitro.

Molecular Therapy, 2013
The bacterial backbone (BB) sequences contained within a canonical plasmid DNA dampen exogenous transgene expression by tenfold to 1,000-fold over a period of a few weeks following transfection into quiescent tissues such as the liver.
Jiamiao Lu, Feijie Zhang, M. Kay
semanticscholar   +1 more source