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Molecular Biotechnology, 2001
A "transgenic" mouse is identified by the integration of a foreign DNA into its genome. Such animals serve as experimental systems for the study of gene expression and are often generated as models for human diseases. Direct microinjection of DNA into the male pronucleus of a mouse zygote has been the method most extensively used in the production of ...
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A "transgenic" mouse is identified by the integration of a foreign DNA into its genome. Such animals serve as experimental systems for the study of gene expression and are often generated as models for human diseases. Direct microinjection of DNA into the male pronucleus of a mouse zygote has been the method most extensively used in the production of ...
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Transgenic Mice With Immunoglobulin Genes
Annual Review of Immunology, 1987Immunoglobulin (Ig) genes encode antibody molecules expressed exclu sively in the B-Iymphocyte lineage. The most immature identifiable cells of the B-cell lineage, pre-B cells, give rise to B cells that differentiate terminally into antibody secreting plasma cells.
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Transgenic RNA Interference in Mice
Physiology, 2007The discovery that small interfering RNA duplexes (siRNA) can silence gene expression in mammalian cells has revolutionized biomedical research. The most successful application of the discovery has been to study gene function in cultured human or mouse cells. However, the knockdown effect of siRNA is only transient.
Xue, Gao, Pumin, Zhang
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Oncogenesis of the Lens in Transgenic Mice
Science, 1987Neoplastic tumors of the ocular lens of vertebrates do not naturally occur. Transgenic mice carrying a hybrid gene comprising the murine αA-crystallin promoter (-366 to +46) fused to the coding sequence of the SV40 T antigens developed lens tumors, which obliterated the eye cavity and even invaded neighboring tissue, thus establishing that the lens is ...
K A, Mahon +5 more
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HBV production in transgenic mice
Gastroenterologia Japonica, 1990We produced transgenic mice by microinjecting a partially duplicated copies of hepatitis B virus (HBV) gene into fertilized eggs of C57BL/6 mice. One mouse was a high producer of HBV surface antigen (HBsAg) and HBV e antigen (HBeAg) in the serum. All offspring carrying HBV DNA were positive for both antigens in the serum.
K, Yamamura +5 more
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Measurement of Anxiety in Transgenic Mice
Reviews in the Neurosciences, 2000A wide range of approaches has been used to study anxiety in mice. All presuppose that aversive stimuli, such as foot shock or novelty, induce a central state of fear, which can be quantified through specific behavioural and physiological measures. This review discusses the validity of the various approaches in terms of their similarity to different ...
S M, Weiss +4 more
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Reporter genes in transgenic mice
Transgenic Research, 1994Although in vivo models utilizing endogenous reporter genes have been exploited for many years, the use of reporter transgenes to dissect biological issues in transgenic animals has been a relatively recent development. These transgenes are often, but not always, of prokaryotic origin and encode products not normally associated with eukaryotic cells ...
C, Cui +4 more
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Insertional mutagenesis in transgenic mice
Transgenic Research, 1994Increasing numbers of transgenic mouse lines have resulted in several dozens of mutants created by insertional mutagenesis. The advantages of different vector systems and the problems associated with the analysis of mutations and the cloning of the affected genes are discussed in this review.
T, Rijkers, A, Peetz, U, Rüther
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Transgenic mice in apoptosis research
Apoptosis, 1998Transgenic mice have proved to be a valuable tool in various aspects of apoptosis research. They are particularly useful for studying apoptosis-related gene products in primary cells which may lead to different effects from similar experiments using immortalized cell lines.
G, Gil-Gómez, H J, Brady
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