Results 171 to 180 of about 16,581 (212)
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Anaemia and Erythrocyte Transketolase Activity
Acta Haematologica, 1972Erythrocyte transketolase activity is raised in megaloblastic anaemia due to vitamin B12 deficiency and inversely related to haemoglobin concentration.
D G, Wells, V, Marks
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Transketolase Activity in Uremia
Archives of Internal Medicine, 1970Erythrocyte transketolase activity (ETKA) was determined in ten uremics undergoing long-term hemodialysis and in four patients treated with Giordano-Giovanetti diet therapy. Transketolase is an essential enzyme in the pentose phosphate shunt and plays a role in myelin sheath maintenance. Its co-factor is thiamine pyrophosphate.
E T, Lonergan, M, Semar, K, Lange
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Donor substrate regulation of transketolase
European Journal of Biochemistry, 2004The influence of substrates on the interaction of apotransketolase with thiamin diphosphate was investigated in the presence of magnesium ions. It was shown that the donor substrates, but not the acceptor substrates, enhance the affinity of the coenzyme either to only one active center of transketolase or to both active centers, but to different ...
Olga A, Esakova +4 more
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Isoforms of baker's yeast transketolase
Experientia, 1970МЕтОДОМ ИОНООБМЕННО И хРОМАтОгРАФИИ с ИспОльжОВАНИЕМ ИОНООБМЕННых сЕФАДЕксОВ пОкАжАНО сУЩЕстВОВАНИЕ ДВУх ИжОФОРМ тРАНскЕтОлАжы пЕкАР скИх ДРОжжЕИ. НАлИЧИЕ ДВУх АктИВНых ИжОФОРМ ФЕРМЕНтА БылО пОДтВЕРжДЕНО тАкжЕ пРИ ЁлЕктРОФОРЕтИЧ ЕскОМ РАжДЕлЕНИИ ВысОкООЧИЩЕННОгО пРЕпАРАтА АпОтРАНс кЕтОлАжы В пОлИАкРИлАМИДНОМ гЕлЕ.
G A, Kochetov, N N, Chernov
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Transketolase stimulation in fibromyalgia.
Journal of the American College of Nutrition, 1990(1990). Transketolase stimulation in fibromyalgia. Journal of the American College of Nutrition: Vol. 9, No. 1, pp. 56-7.
J, Eisinger, T, Ayavou
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Variants of transketolase from human erythrocytes
Clinica Chimica Acta, 1983Analytical isoelectric focusing and a stain for transketolase have been applied to partially purified samples of human erythrocyte hemolysates and have detected individual species of transketolase having pI values of 6.6, 7.3, 7.5, 7.8, 8.1, 8.2, 8.4 and 9.2. Six different patterns of these species were detected in 25 healthy subjects.
Kaczmarek, M.Jan, Nixon, Peter F.
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Kinetic Studies of Mouse Brain Transketolase
Journal of Neurochemistry, 1982Abstract: The activity of transketolase in mouse brain was 5.7 nmol/min/mg protein measured by an enzyme‐coupled spectrophotometric assay. The apparent Km for ribose‐5‐phosphate was 330 μM, for d‐xylulose‐5‐phosphate was 120 μM, and for thiamine pyrophosphate was 7 μM.
J P, Blass +3 more
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Inhibition of transketolase by hexacyanoferrate(III)
Biochemistry (Moscow), 2010The effect of hexacyanoferrate(III) on the catalytic activity of transketolase has been studied. This oxidant inactivates only one of two active sites of the enzyme, the one with a higher affinity to the coenzyme (thiamine diphosphate). The second active site does not lose its catalytic activity.
V A, Yurshev +3 more
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The Catalytic Mechanism of Human Transketolase
ChemPhysChem, 2019AbstractWe have computationally determined the catalytic mechanism of human transketolase (hTK) using a cluster model approach and density functional theory calculations. We were able to determine all the relevant structures, bringing solid evidences to the proposed experimental mechanism, and to add important detail to the structure of the transition ...
Prejano M. +5 more
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Bioscience, Biotechnology, and Biochemistry, 1993
Given the importance of transketolase, TK (EC 2.2.1.1) for both pharmacological studies and synthetic purposes, the need for a simple and inexpensive assay is patent. We describe here a simple and inexpensive TK assay using the unphosphorylated ketoses L-erythrulose, 4-deoxy-L-erythrulose, and 4-deoxy-D,L-erythrulose as donor substrates instead of D ...
Laurence Hecquet +2 more
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Given the importance of transketolase, TK (EC 2.2.1.1) for both pharmacological studies and synthetic purposes, the need for a simple and inexpensive assay is patent. We describe here a simple and inexpensive TK assay using the unphosphorylated ketoses L-erythrulose, 4-deoxy-L-erythrulose, and 4-deoxy-D,L-erythrulose as donor substrates instead of D ...
Laurence Hecquet +2 more
openaire +1 more source