Results 171 to 180 of about 10,904 (208)
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Donor substrate regulation of transketolase
European Journal of Biochemistry, 2004The influence of substrates on the interaction of apotransketolase with thiamin diphosphate was investigated in the presence of magnesium ions. It was shown that the donor substrates, but not the acceptor substrates, enhance the affinity of the coenzyme either to only one active center of transketolase or to both active centers, but to different ...
Olga A, Esakova +4 more
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Structure and functioning mechanism of transketolase
Biochimica et Biophysica Acta (BBA) - Proteins and Proteomics, 2014Studies of thiamine diphosphate-dependent enzymes appear to have commenced in 1937, with the isolation of the coenzyme of yeast pyruvate decarboxylase, which was demonstrated to be a diphosphoric ester of thiamine. For quite a long time, these studies were largely focused on enzymes decarboxylating α-keto acids, such as pyruvate decarboxylase and ...
German A, Kochetov, Olga N, Solovjeva
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Sulfhydryl groups and transketolase activity
Biochemical and Biophysical Research Communications, 1966Abstract Transketolase, an enzyme of pentose cycle, catalyzes the reaction of glycolaldehyde transport from the xylulose-5-phosphate to the ribose-5-phosphate with the formation of sedoheptulose-7-phosphate. There is not much known about this enzyme, especially in respect to its functional groups.
G A, Kochetov, F G, Lutovinova
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Functional Flexibility of the Transketolase Molecule
Biochemistry (Moscow), 2001Transketolase is the simplest representative of the thiamine diphosphate-dependent enzymes. It was the first of these enzymes for which X-ray analysis was performed. Based on the data of X-ray studies and using the mutagenesis technique, the nature of functional groups of the enzyme involved in the interaction with substrates and cofactors and in the ...
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Anaemia and Erythrocyte Transketolase Activity
Acta Haematologica, 2009Erythrocyte transketolase activity is raised in megaloblastic anaemia due to vitamin B<sub>12</sub> deficiency and inversely related to haemoglobin concentration. It si usually normal in cases of megaloblastic anaemia due to folate deficiency though sufficient exceptions occur to invalidate the use of red cell transketolase assay in ...
D G, Wells, V, Marks
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Applications of transketolases in organic synthesis
Current Opinion in Biotechnology, 2000The enzyme transketolase has been employed as a catalyst for asymmetric carbon-carbon bond formation in the synthesis of biologically important molecules. A number of important parameters have been addressed including substrate specificity, over-expression of the protein in suitable host systems, scale-up of the reaction and use of transketolase in ...
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Erythrocyte Transketolase Activity
The American Journal of Clinical Nutrition, 1970Y H, Chong, G S, Ho
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Transketolase enzyme discovery and engineering
Transketolases (TKs) are important C-C bond forming enzymes that in vivo transfer a two carbon ketol unit to the acceptors d-ribose-5-phosphate or d-erythrose-4-phosphate. There is significant interest in biocatalytic applications where frequently the donor β-hydroxypyruvic acid is used.Alessia, Tonoli +3 more
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