Results 111 to 120 of about 287,743 (162)

lacZ translation initiation mutations

Journal of Molecular Biology, 1984
Sixteen single point mutations near the beginning of the lacZ gene have been isolated and their effect on lacZ expression has been measured. Five mutations were obtained that alter a potential stem-and-loop structure in the messenger RNA that masks the initiation codons.
L M, Munson, G D, Stormo, R L, Niece, W S, Reznikoff   +3 more
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Translational Initiation in Prokaryotes

Annual Review of Microbiology, 1981
INTRODUCTION 365 BIOCHEMISTRY OF TRANSLATIONAL INITIATION 367 A CONCEPTUAL FRAMEWORK: RIBOSOME BINDING SITE STRENGTHS 373 THE NATURE OF RIBOSOME BINDING SITES 374 A First Approximation 374 Three Approaches to a Higher Order Approximation 380 Genetics and biochemistry 380 Statistics .......•••••••••.••..•.••.•...•.•........•....••••••••
L, Gold, D, Pribnow, T, Schneider, S, Shinedling, B S, Singer, G, Stormo   +5 more
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Translation initiation factor 4E

The International Journal of Biochemistry & Cell Biology, 1999
Translation initiation factor 4E (eIF4E) binds the 7-methylguanosine cap structure of mRNA and mediates recruitment of mRNA to ribosomes, with the potential of regulating the overall rate of translation and discriminating between different RNAs. Increased translation is required for progress through the cell cycle, and it is therefore not surprising ...
L, McKendrick, V M, Pain, S J, Morley
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Internal initiation of translation

Current Opinion in Cell Biology, 1993
Although the 5' cap-dependent scanning mechanism can account for the translational initiation of most mRNAs in eukaryotic cells, several viral and cellular mRNAs contain nucleotide sequences in their 5' non-coding regions that can mediate binding of ribosomes to the mRNA, regardless of the modification state of the 5' ends.
S, McBratney, C Y, Chen, P, Sarnow
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Translation initiation and viral tricks

Trends in Biochemical Sciences, 2003
A variety of viral strategies are utilized for dominance of the host-cell protein synthetic machinery, optimization of viral mRNA translation and evasion of host-cell antiviral responses that act at the translational level. Many viruses exploit regulated steps in the initiation of cellular protein synthesis to their own advantage.
Robert J, Schneider, Ian, Mohr
openaire   +2 more sources

Translation | Translation Initiation in Bacteria: Factors and Mechanisms

2021
Bacterial translation initiation pathway entails several steps kinetically controlled by initiation factors IF1, IF2 and IF3. The pathway begins with formation of the 30S pre-initiation complex containing all 30S ligands followed by a locking step stabilizing the P-site decoding of the mRNA initiation triplet (AUG in most cases) by fMet-tRNAfmet ...
Gualerzi C. O., Giuliodori A. M.
openaire   +1 more source

Translation initiation in colorectal cancer

Cancer and Metastasis Reviews, 2012
Colorectal cancers (CRC) are one of the most common causes of morbidity and mortality in high-income countries. Targeted screening programs have resulted in early treatment and a substantial decrease in mortality. However, treatment strategies for CRC still require improvement.
Armen, Parsyan, Greco, Hernández, Sarkis, Meterissian   +2 more
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Reconstitution of Yeast Translation Initiation

2007
To facilitate the mechanistic dissection of eukaryotic translation initiation we have reconstituted the steps of this process using purified Saccharomyces cerevisiae components. This system provides a bridge between biochemical studies in vitro and powerful yeast genetic techniques, and complements existing reconstituted mammalian translation systems ...
Michael G, Acker, Sarah E, Kolitz, Sarah F, Mitchell, Jagpreet S, Nanda, Jon R, Lorsch   +4 more
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Translation initiation: adept at adapting

Trends in Biochemical Sciences, 1999
Initiation of protein synthesis requires both an mRNA and the initiator methionyl (Met)-tRNA to be bound to the ribosome. Most mRNAs are recruited to the ribosome through recognition of the 5' m7G cap by a group of proteins referred to as the cap-binding complex or eIF4F.
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Translation Enhancer Improves the Ribosome Liberation from Translation Initiation

Journal of the American Chemical Society, 2013
For translation initiation in bacteria, the Shine-Dalgarno (SD) and anti-SD sequence of the 30S subunit play key roles for specific interactions between ribosomes and mRNAs to determine the exact position of the translation initiation region. However, ribosomes also must dissociate from the translation initiation region to slide toward the downstream ...
Shuntaro, Takahashi, Hiroyuki, Furusawa, Takuya, Ueda, Yoshio, Okahata   +3 more
openaire   +2 more sources