Yeast applied readthrough inducing system (YARIS): an invivo assay for the comprehensive study of translational readthrough. [PDF]
Nucleic Acids Res, 2019 AbstractStop codon readthrough—the decoding of a stop codon by a near-cognate tRNA—is employed by viruses to balance levels of enzymatic and structural proteins and by eukaryotic cells to enable isoform-specific protein synthesis in response to external stimuli.
Beznosková P +4 more
europepmc +7 more sources
Advances in therapeutic use of a drug-stimulated translational readthrough of premature termination codons [PDF]
Molecular Medicine, 2018 Premature termination codons (PTCs) in the coding regions of mRNA lead to the incorrect termination of translation and generation of non-functional, truncated proteins.
Maciej Dabrowski +2 more
doaj +2 more sources
Translational readthrough in Tobacco necrosis virus-D
Virology, 2014 The plus-strand RNA genome of Tobacco necrosis virus-D (TNV-D) expresses its polymerase via translational readthrough. The RNA signals involved in this readthrough process were characterized in vitro using a wheat germ extract translation system and in vivo via protoplast infections.
Newburn, Laura R. +4 more
openaire +4 more sources
Anticodon-edited tRNA enables translational readthrough of COL4A5 premature termination codons. [PDF]
PLoS ONEAlport syndrome is caused by variants in COL4A3, COL4A4, or COL4A5, which encode the α3α4α5 chains of type IV collagen. These variants result in defects in the glomerular basement membrane (GBM) and impaired kidney function.
Kohei Omachi +3 more
doaj +2 more sources
Translational readthrough therapy for ADPKD induces polycystin1 expression and partially rescues functional deficits in PKD1 mutant cells [PDF]
Scientific ReportsAutosomal-Dominant Polycystic Kidney Disease, ADPKD, is the most common genetic kidney disease affecting 1:1000 people worldwide. It is caused by mutations in the PKD1 (~ 80%) or PKD2 gene (~ 15%).
Elena Torban +7 more
doaj +2 more sources
Cell, 2023 Readthrough into the 3' untranslated region (3' UTR) of the mRNA results in the production of aberrant proteins. Metazoans efficiently clear readthrough proteins, but the underlying mechanisms remain unknown. Here, we show in Caenorhabditis elegans and mammalian cells that readthrough proteins are targeted by a coupled, two-level quality control ...
Muller, Martin B. D. +3 more
openaire +7 more sources
Therapeutic Opportunities in Overcoming Premature Termination Codons in Epidermolysis Bullosa via Translational Readthrough [PDF]
CellsEpidermolysis Bullosa (EB) comprises a group of inherited blistering disorders caused by pathogenic variants in genes essential for skin and mucosal integrity.
Kathleen L. Miao +3 more
doaj +2 more sources
Genetic basis of hidden phenotypic variation revealed by increased translational readthrough in yeast. [PDF]
PLoS Genetics, 2012 Eukaryotic release factors 1 and 3, encoded by SUP45 and SUP35, respectively, in Saccharomyces cerevisiae, are required for translation termination. Recent studies have shown that, besides these two key factors, several genetic and epigenetic mechanisms ...
Noorossadat Torabi, Leonid Kruglyak
doaj +6 more sources
Seryl-tRNA synthetase promotes translational readthrough by mRNA binding and involvement of the selenocysteine incorporation machinery. [PDF]
Nucleic Acids Res, 2023 Liu Z +12 more
europepmc +2 more sources
Readthrough Approach Using NV Translational Readthrough-Inducing Drugs (TRIDs): A Study of the Possible Off-Target Effects on Natural Termination Codons (NTCs) on TP53 and Housekeeping Gene Expression. [PDF]
Int J Mol Sci, 2023 Perriera R +11 more
europepmc +2 more sources