Results 51 to 60 of about 5,883 (112)

Stops making sense: translational trade-offs and stop codon reassignment

BMC Evolutionary Biology, 2011
Background Efficient gene expression involves a trade-off between (i) premature termination of protein synthesis; and (ii) readthrough, where the ribosome fails to dissociate at the terminal stop.
Elgar Greg S, Lichtenstein Conrad P, Cotton James A, Johnson Louise J, Nichols Richard A, Polly p David, Le Comber Steven C   +6 more
doaj   +1 more source

Distal nucleotides affect the rate of stop codon read‐through

Quantitative Biology, 2023
Background A key step in gene expression is the recognition of the stop codon to terminate translation at the correct position. However, it has been observed that ribosomes can misinterpret the stop codon and continue the translation in the 3′UTR region.
Luciana I. Escobar, Andres M. Alonso, Jorge R. Ronderos, Luis Diambra   +3 more
doaj   +1 more source

A Modular Mechanistic In Silico Model for In Vitro Transcription Process Yield and Product Quality Prediction

Biotechnology and Bioengineering, Volume 123, Issue 7, Page 1836-1870, July 2026.
ABSTRACT In vitro transcription (IVT) plays a critical role in the manufacture of mRNA vaccines and therapeutics. Optimizing mRNA yield and ensuring product quality, such as capping efficiency and integrity, are essential but mechanistically complex. This study presents a modular mechanistic model of the IVT process to advance scientific understanding ...
Keqi Wang, Keilung Choy, Eli Reiser, Jinxiang Pei, Hua Zheng, Aparajita Dasgupta, Fuqiang Cheng, Guogang Dong, Bhanu Chandra Mulukutla, Joshua Mannheimer, Carolyn Huang, Hooman Farsani, Wei Xie   +12 more
wiley   +1 more source

Ribosome profiling of the retrovirus murine leukemia virus

Retrovirology, 2018
Background The retrovirus murine leukemia virus (MuLV) has an 8.3 kb RNA genome with a simple 5′-gag-pol-env-3′ architecture. Translation of the pol gene is dependent upon readthrough of the gag UAG stop codon; whereas the env gene is translated from ...
Nerea Irigoyen, Adam M. Dinan, Ian Brierley, Andrew E. Firth   +3 more
doaj   +1 more source

NaP‐TRAP: A Versatile and Accessible Workflow to Dissect Principles of Translational Regulation and mRNA Stability

Current Protocols, Volume 6, Issue 7, July 2026.
Abstract The translation of messenger RNA (mRNA) into protein is tightly regulated by both cellular trans‐factors and cis‐regulatory elements encoded within transcripts. Although transcript fate can be measured by transcript abundance or translation efficiency, separating the contribution of individual cis‐element within a single transcript is an ...
Amit Gupta, Anna Z. Struba, Srihari Madhavan, Ethan Strayer, Jean‐Denis Beaudoin   +4 more
wiley   +1 more source

Detection and molecular characterisation of beet chlorosis virus in sugar beet in the Czech Republic

Annals of Applied Biology, Volume 189, Issue 1, July 2026.
Beet chlorosis virus (BChV) was detected for the first time in Czech sugar beet, and its diversity was assessed. One‐enzyme RTX‐PCR screened 37 symptomatic plants from 15 fields, and BChV was found in 12 plants. Twelve isolates were confirmed by Sanger sequencing of the readthrough domain (RTD), while one representative isolate was sequenced by high ...
Emad Ibrahim, Ali Pasha, Heiko Ziebell, Marie Maňasová, Pavel Ryšánek, Jiban K. Kundu   +5 more
wiley   +1 more source

Therapeutic targeting of TP53 nonsense mutations in cancer

Upsala Journal of Medical Sciences
Mutations in the TP53 tumor suppressor gene occur with high prevalence in a wide range of human tumors. A significant fraction of these mutations (around 10%) are nonsense mutations, creating a premature termination codon (PTC) that leads to the ...
Charlotte Strandgren, Klas G. Wiman
doaj   +1 more source

Potato leafroll virus: A re‐emerging threat to sustainable potato production

Annals of Applied Biology, Volume 189, Issue 1, July 2026.
Potato leafroll virus (PLRV) is a positive‐strand RNA virus. Virus particles of PLRV are isometric, 24 nm in diameter. PLRV is the causal agent of leafroll disease in potato (Solanum tuberosum L.) which causes significant losses in yield and quality of potato tubers.
Eugene V. Ryabov, Andrew Love, Lesley Torrance   +2 more
wiley   +1 more source

Salt Stress Adaptations in Soybean Involve Alterations in Pre‐mRNA Processing

Plant, Cell &Environment, Volume 49, Issue 7, Page 4000-4014, July 2026.
ABSTRACT Salt stress can seriously affect plant survival. To adapt to salt stress, plants can alter gene expressions and/or pre‐mRNA processing patterns, or both. Previous studies could not comprehensively profile stress‐responsive pre‐mRNA processing patterns due to limitations in traditional sequencing technologies.
Shoudong Zhang, Zhixia Xiao, Ailin Liu, Dongpeng Ren, Shengjie Chen, Hanxue Zhang, Li Zhang, Zhili Wang, Jun Yang, Hon‐Ming Lam   +9 more
wiley   +1 more source

RATEX: A Scalable RNA‐Based Platform for Logical and Multi‐Layered Cellular Programming

Angewandte Chemie, Volume 138, Issue 26, 22 June 2026.
RATEX integrated ribosome‐mediated transcription control with synthetic RNA regulators, enabling simultaneous processing of RNA, metabolite, and small‐molecule inputs through complex logic operations within a single transcript. The platform achieves up to 1,492‐fold gene regulation, multi‐input hybrid logic gates, RNA‐programmed signaling cascades, and
Hyunseop Goh, Hansol Kang, Chaeri Kim, Jongmin Kim   +3 more
wiley   +1 more source