Results 151 to 160 of about 68,477 (364)

Analyzing accessible chromatin with mua transposase.

open access: yes, 2020
Open chromatin analysis uses Mnase-seq, Chip-seq, and DNase-seq methods, which can detect epigenetic changes and provide information on transcription factor binding sites, chromatin availability at promoters, enhancers, or other regulatory elements ...
Stragis, Martynas,
core  

A Domesticated PiggyBac Transposase Interacts with Heterochromatin and Catalyzes Reproducible DNA Elimination in Tetrahymena

open access: yesPLoS Genetics, 2013
The somatic genome of the ciliated protist Tetrahymena undergoes DNA elimination of defined sequences called internal eliminated sequences (IESs), which account for ∼30% of the germline genome.
Alexander Vogt, Kazufumi Mochizuki
semanticscholar   +1 more source

Eco‐Geography Reverses Dominant AMR Reservoirs in Klebsiella pneumoniae: Integron‐Rich Mobilomes and Cross‐Niche Connectivity

open access: yesAdvanced Science, EarlyView.
Dominant antimicrobial resistance reservoirs in Klebsiella pneumoniae vary across eco‐geographic settings rather than following a universal pattern. Integrated One Health and global genomic analyses show that lineage structure, integron load, and cross‐niche connectivity shape whether AMR burden accumulates primarily in human or nonhuman compartments ...
Hui Lin   +12 more
wiley   +1 more source

Supplementary Information from Biochemical analysis of nucleosome targeting by Tn5 transposase

open access: yes, 2019
Tn5 transposase is a bacterial enzyme that integrates a DNA fragment into genomic DNA, and is used as a tool for detecting nucleosome-free regions of genomic DNA in eukaryotes.
Tomoya Kujirai (3915680)   +7 more
core   +2 more sources

Transcriptional activity of PIF and Pong-like Class II transposable elements in Triticeae

open access: yesBMC Evolutionary Biology, 2017
Background Transposable elements are major contributors to genome size and variability, accounting for approximately 70–80% of the maize, barley, and wheat genomes.
Dragomira N. Markova   +1 more
doaj   +1 more source

The Human THAP9 Gene Encodes an Active P-Element DNA Transposase

open access: yesScience, 2013
Mobilizing Transposable Elements Transposable elements are parasitic DNA elements that can jump around the genome. They make up large portions of eukaryotic genomes (up to 50% of the human genome).
S. Majumdar, Anita Singh, D. Rio
semanticscholar   +1 more source

Transposase‐Assisted Donor Tethering Boosts Large‐Fragment HDR in Plants

open access: yesAdvanced Science, EarlyView.
A transposase‐assisted donor tethering strategy is developed to enhance homology‐directed repair in plants. By recruiting donor DNA to double‐strand breaks and synergizing with repair pathway reprogramming and transcription‐coupled donor design, this system markedly improves large‐fragment targeted insertion efficiency, providing a robust platform for ...
Sha Wei   +8 more
wiley   +1 more source

Improving genetic transformation rates in honeybees

open access: yesScientific Reports, 2018
Functional genetic studies in honeybees have been limited by transformation tools that lead to a high rate of transposon integration into the germline of the queens.
M. Otte   +5 more
doaj   +1 more source

A hybrid lentivirus-transposon vector for safer gene therapy

open access: yes, 2010
Gene therapy vectors based on the HIV-1 lentivirus are an attractive option for clinical applications because they enter a broad range of target cells efficiently and deliver stable gene expression through integration into host chromosomes.
Vink, C.A.
core  

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