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Capturing transposases for new proteins
Nature Reviews Genetics, 2021A new study by Cosby et al., investigating the role of DNA transposable elements in exon shuffling, reports that transposase capture is a recurrent process in evolution underlying the origin of new regulators of gene expression.
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Overproduction and Purification of the Tn3 Transposase
The Journal of Biochemistry, 1987The Tn3 transposase accumulated to about 4% of total cell protein in a minicell-producing Escherichia coli strain harboring a transposase overproducer plasmid. This accumulation of the transposase seems to be due to four factors: derepression of transcription resulting from inactivation of the repressor gene (tnpR); efficient translation caused by a ...
M, Morita, S, Tsunasawa, Y, Sugino
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Induction of the SOS Response by IS1 Transposase
Journal of Molecular Biology, 1994We find that IS1 transposase, like that of Tn10, can induce the SOS response when produced at high levels. Most of the activity (> 80%) requires IS1 ends in cis to the transposase gene and depends strictly on the presence of RecBCD function. This implies that processing of transposase-induced cleavages is responsible for generating the response ...
David Lane +2 more
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Molecular Microbiology, 1996
Summary The transposase protein encoded by the insertion element IS 903 belongs to an unusual class of DNA‐binding proteins, termed cis‐acting proteins, that act preferentially at their site of synthesis. Previous work had led us to propose that instability of the IS 903 transposase was a major determinant of its cis preference.
K M, Derbyshire, N D, Grindley
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Summary The transposase protein encoded by the insertion element IS 903 belongs to an unusual class of DNA‐binding proteins, termed cis‐acting proteins, that act preferentially at their site of synthesis. Previous work had led us to propose that instability of the IS 903 transposase was a major determinant of its cis preference.
K M, Derbyshire, N D, Grindley
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Defining functional regions of the IS903 transposase
Journal of Molecular Biology, 1997The insertion sequence IS903 encodes a 307 amino acid residue protein, transposase, that is essential for transposition. It is a multi-functional DNA-binding protein that specifically recognizes the 18 bp inverted repeats at the ends of the element and also recognizes DNa non-specifically when it captures a target site.
Keith M Derbyshire
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Hyperactive piggyBac transposase improves transformation efficiency in diverse insect species.
Insect Biochemistry and Molecular Biology, 2018Even in times of advanced site-specific genome editing tools, the improvement of DNA transposases is still on high demand in the field of transgenesis: especially in emerging model systems where evaluated integrase landing sites have not yet been created
K. Eckermann +7 more
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