Results 331 to 340 of about 68,477 (364)

Transposase-driven rearrangements in human tumors

Nature Genetics, 2017
A new study shows that aberrant DNA transposase activity promotes structural alterations that are clonally selected to drive tumor development. This discovery uncovers novel mechanisms of tumor-suppressor gene inactivation and highlights a new approach to cancer gene identification.
Stephen C, Mack, Hiromichi, Suzuki, Michael D, Taylor   +2 more
openaire   +2 more sources

Interaction of Tn5 Transposase with the Transposon Termini

Journal of Molecular Biology, 1994
Transposition of Tn5 requires the binding of the transposase protein to the transposon outside end (OE) DNA sequences. Transposase mutants that increase the transposition frequency result in the formation of two distinct transposase/OE DNA complexes, observed by gel retardation analysis.
T W, Wiegand, W S, Reznikoff
openaire   +2 more sources

Evidence for “Unseen” Transposase–DNA Contacts

Journal of Molecular Biology, 2002
In this study, evidence of novel, important interactions between a hyperactive Tn5 transposon recognition end sequence and hyperactive Tn5 transposase (Tnp) are presented. A hyperactive Tn5 end sequence, the mosaic end (ME), was isolated previously.
Mindy, Steiniger-White, Archna, Bhasin, Scott, Lovell, Ivan, Rayment, William S, Reznikoff   +4 more
openaire   +2 more sources

DNA binding domains in Tn3 transposase

Molecular and General Genetics MGG, 1993
Various segments of Tn3 transposase were fused individually to beta-galactosidase, and the resulting fusion proteins were examined for their DNA binding ability by a nitrocellulose filter binding assay. Analyses of a series of the fusion proteins revealed that the N-terminal segment of the transposase (amino acid positions 1-242; the transposase gene ...
T, Maekawa, J, Amemura-Maekawa, E, Ohtsubo   +2 more
openaire   +2 more sources

The role of TnA transposase in transposition immunity

Plasmid, 1984
The strength of expression of transposition immunity depends on the level of transposase available in the system, and on the location of the element to be transposed.
J, Heritage, P M, Bennett
openaire   +2 more sources

Stoichiometric use of the transposase of bacteriophage Mu

Cell, 1984
The transposase of bacteriophage Mu (gene A protein) mediates the coupled replication and integration processes that constitute transposition during the lytic cycle. Our previous results showed that the activity of the A protein is unstable, as its continued synthesis is required to maintain Mu DNA replication throughout the lytic cycle.
M L, Pato, C, Reich
openaire   +2 more sources

Integrating DNA: Transposases and Retroviral Integrases

Annual Review of Microbiology, 1999
▪ Abstract  Transposable elements appear quite disparate in their organization and in the types of genetic rearrangements they promote. In spite of this diversity, retroviruses and many transposons of both prokaryotes and eukaryotes show clear similarities in the chemical reactions involved in their transposition.
L, Haren, B, Ton-Hoang, M, Chandler
openaire   +2 more sources

Molecular architecture of a eukaryotic DNA transposase

Nature Structural & Molecular Biology, 2005
Mobile elements and their inactive remnants account for large proportions of most eukaryotic genomes, where they have had central roles in genome evolution. Over 50 years ago, McClintock reported a form of stress-induced genome instability in maize in which discrete DNA segments move between chromosomal locations.
Alison B, Hickman, Zhanita N, Perez, Liqin, Zhou, Primrose, Musingarimi, Rodolfo, Ghirlando, Jenny E, Hinshaw, Nancy L, Craig, Fred, Dyda   +7 more
openaire   +2 more sources

Repression of the Ac‐transposase gene promoter by Ac transposase

The Plant Journal, 1996
SummaryMobility of the maize Ac‐Ds transposable element family depends on the production of Ac‐encoded transposase (TPASE). The TPASE is a DNA‐binding protein which recognizes internal sites near both Ac termini in a region which overlaps the putative TPASE gene promoter. Therefore, it was hypothesized that TPASE may regulate its own transcription. The
Marcelo Fridlender, Kate Harrison, Jonathan D.G. Jones, Avraham A. Levy   +3 more
openaire   +1 more source

Factors that affect transposition mediated by the Tn21 transposase

Plasmid, 1988
The frequencies of one-ended transposition mediated by the Tn21 transposase acting on plasmids containing 38-bp inverted repeat sequences (IRs) of both Tn21 and of Tn501/Tn1721 and Tn2501 were measured. The enzyme acted on all these IRs, but more efficiently on the homologous sequences. These differences were magnified when the enzyme acted on plasmids
J, Grinsted, C, Martin, F, de la Cruz
openaire   +2 more sources