Loop‐mediated isothermal amplification (LAMP) is a quick, cost‐effective molecular technique for detecting bacterial pathogens causing bovine mastitis. This review summarises 18 LAMP assays developed for key bacteria such as Staphylococcus aureus, Streptococcus agalactiae, Streptococcus uberis and Escherichia coli.
Tewodros Fentahun Jember +3 more
wiley +1 more source
Purification and properties of trehalase(s) from Neurospora [PDF]
Trehalase has been obtained in crystalline form from the mycelium of Neurospora crassa. During elution from the first pass through a DEAE-cellulose column, two peaks with trehalase activity were obtained.
Sussman, Alfred S., Hill, E. P.
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SUMMARY Plants face constant environmental changes and must integrate external and internal cues to coordinate growth, development, reproduction, and stress responses. A major strategy is perception at the cell surface via a large, diverse network of receptors.
Bruno Pok Man Ngou +2 more
wiley +1 more source
Determination of Trehalose by Flow Injection Analysis Using Immobilized Trehalase [PDF]
Meyer zu Düttingdorf H-D, Bachmann B, Buchholz M, Leuchtenberger W. Determination of Trehalose by Flow Injection Analysis Using Immobilized Trehalase. Analytical Biochemistry.
Bachmann, B. +3 more
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Osmo-stress-induced changes in neutral trehalase activity of the fission yeast Schizosaccharomyces pombe [PDF]
Exposure of repressed growing cultures of Schizosaccharomyces pombe to various extracellular concentrations of NaCl, sorbitol or glycerol resulted in a reversible increase in neutral trehalase activity which was maintained while the cells were in the ...
Gacto, M +9 more
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Trehalase expression during the germination of three resting stages of the dictyosteliaceae [PDF]
Three resting stages (spores, microcysts, and macrocysts) of the Dictyosteliaceae were found to possess low levels of trehalase activity. A significant increase in the activity of trehalase was detected upon germination. The increase in activity appeared
Tisa, Louis S., Cotter, David A.
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Distribution, Purification, and Characterization of Trehalase from the Fruit Body of King Oyster Mushroom, Pleurotus eryngii [PDF]
P(論文)Trehalase activity in a homogenized preparation from the fruit body of king oyster mushroom (eryngii), Pleurotus eryngii was studied in detail. Distribution of trehalose and trehalase activity was higher in the cap (pileus) fraction than in the ...
竹内, 梓 +43 more
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Activation of neutral trehalase by glucose and nitrogen source in Schizosaccharomyces pombe strains deficient in cAMP-dependent protein kinase activity [PDF]
Schizosaccharomyces pombe cells carrying a disruption in the PKAI gene, that encodes the catalytic subunit of cAMP-dependent protein kinase (PKA), lacked the glucose- and nitrogen-source-induced activation of trehalase at stationary-phase but rised ...
Gacto, M. +9 more
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Molecular characterization of the Aspergillus nidulans treA gene encoding an acid trehalase required for growth on trehalose [PDF]
International audienceAspergillus nidulans conidiospores contain high levels of the non‐reducing disaccharide trehalose. We show that upon induction of conidiospore germination, the trehalose pool is rapidly degraded and a glycerol pool is transiently ...
d'Enfert, Christophe, Fontaine, Thierry
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Histochemical localization of trehalase activity in dorsal flight muscle of the flesh fly Sarcophaga bullata with light and electron microscopy. [PDF]
Trehalase activity in flight muscle of the flesh fly Sacrophaga bullata is detected histochemically at light- and electron-microscopic levels by using diaminobenzidine, glucose oxidase and peroxidase in the incubation medium.
P E Morrison, P L Chang
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