Results 131 to 140 of about 11,664 (177)

Conversion of a Mono‐ and Diacylglycerol Lipase into a Triacylglycerol Lipase by Protein Engineering

ChemBioChem, 2015
AbstractDespite the fact that most lipases are believed to be active against triacylglycerides, there is a small group of lipases that are active only on mono‐ and diacylglycerides. The reason for this difference in substrate scope is not clear. We tried to identify the reasons for this in the lipase from Malassezia globosa. By protein engineering, and
Lan, D., Popowicz, G.M., Pavlidis, I.V., Zhou, P., Bornscheuer, U.T., Wang, Y.   +5 more
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Triacylglycerol metabolism and triacylglycerol lipase activities of cultured human skin fibroblasts

Biochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism, 1980
The relative contribution of lipoproteins and free fatty acid to the cellular triacylglycerol content of cultured human fibroblasts was tested. Fibroblasts accumulated triacylglycerol in proportion to the molar ratio of free fatty acid (oleic acid) to albumin in the medium.
J F, Oram, E, Shafrir, E L, Bierman
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Identification of a triacylglycerol lipase in the diatom Phaeodactylum tricornutum

Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids, 2016
Diatoms accumulate triacylglycerols (TAGs) as storage lipids, but the knowledge about the molecular mechanisms of lipid metabolism is still sparse. Starting from a partial sequence for a putative TAG-lipase of the diatom Phaeodactylum tricornutum retrieved from the data bases, we have identified the full length coding sequence, tgl1.
Barka, F., Angstenberger, M., Ahrendt, T., Lorenzen, W., Bode, H., Buechel, C.   +5 more
openaire   +3 more sources

Some properties of triacylglycerol lipase in chicken erythrocytes

International Journal of Biochemistry, 1986
Membrane-bound acid lipase was found in the chicken erythrocytes ghosts, having an optimum pH of 4.5. The membrane-bound lipase showed its maximum activity at 38 degrees C, and it was stable below 45 degrees C. The bound lipase was activated by octyl glucoside and 3-[(3-cholamidopropyl)dimethylammonio]-1-propanesulfonate (CHAPS), but it was markedly ...
M, Fujii, S, Nishisako, T, Fukunaga, K, Koga   +3 more
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Regulation and function of triacylglycerol lipases in cellular metabolism

Biochemical Journal, 2008
The ability to store energy in the form of energy-dense TAG (triacylglycerol) and to mobilize these stores rapidly during times of low carbohydrate availability (fasting or famine) or during heightened metabolic demand (exercise or cold-stress) is a highly conserved process essential for survival. Today, in the presence of nutrient excess and sedentary
Matthew J, Watt, Gregory R, Steinberg
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Hydrolysis of synthetic triacylglycerols by pancreatic and lipoprotein lipase

Lipids, 1974
AbstractThe stereochemical course of the hydrolysis of synthetic sn‐glycerol‐1‐palmitate‐2‐oleate‐3‐linoleate, sn‐glycerol‐1,2‐dipalmitate‐3‐oleate and their antipodes by pancreatic and milk lipoprotein lipase was investigated by thin layer and gas liquid chromatographies of the diacylglycerol intermediates.
N H, Morley, A, Kuksis, D, Buchnea
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Phosphonate analogues of triacylglycerols are potent inhibitors of lipase

Biochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism, 1995
1,2-Dioctylcarbamoylglycero-3-O-p-nitrophenyl alkylphosphonates, with alkyl being methyl or octyl, were synthesised and tested as irreversible inhibitors of cutinase from Fusarium solani pisi and Staphylococcus hyicus lipase. Rapid inactivation of these enzymes occurred with a concomitant release of one mole of p-nitrophenol per mole of enzyme.
M L, Mannesse, J W, Boots, R, Dijkman, A J, Slotboom, H T, van der Hijden, M R, Egmond, H M, Verheij, G H, de Haas   +7 more
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Hydrolysis of emulsified mixtures of triacylglycerols by pancreatic lipase

Biochimica et Biophysica Acta (BBA) - Protein Structure and Molecular Enzymology, 1999
Hydrolysis of the emulsified mixture of short-chain triacylglycerols by porcine pancreatic lipase in the presence of procolipase and micellar sodium taurodeoxycholate has been studied. Increase in the content of tributyrin and trioctanoin in the mixture with triacetin had highly cooperative effects on the formation of the interfacial lipase procolipase
T, Käämbre, V, Tõugu, P, Käämbre, H, Vija, P, Sikk   +4 more
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On the Similarity of Triacylglycerol and Acylcholesterol Lipases in Rat Brain

Journal of Neurochemistry, 1983
Abstract: Previously, we described a similar stimulating effect of adrenocorticotrophic hormone (ACTH) and ACTH‐related synthetic peptides on triacylglycerol (TAGL) and acylcholesterol (ACL) lipase activities of rat brain. In present study, TAGL and ACL activities from rat brain were further investigated and compared through the use of tri‐[3H ...
J, Arnaud, O, Nobili, J, Boyer
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Effect of cholera toxin on triacylglycerol lipase activity and triacylglycerol content of rat heart

Canadian Journal of Physiology and Pharmacology, 1987
This study was performed to reexamine the effect of cholera toxin on total and intracellular alkaline lipoprotein lipase (LPL) activity in rat heart. In addition, the relationship between intracellular triacylglycerol (TG)lipase activity and TG content of cardiac tissue was determined in cholera toxin treated rats. One intravenous injection of cholera
W C, Miller, W K, Palmer, D A, Arnall, L B, Oscai   +3 more
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