Results 141 to 150 of about 11,664 (177)

Regioselective analysis of triacylglycerols by lipase hydrolysis

Journal of the American Oil Chemists' Society, 1995
AbstractA modified procedure for the regiospecific analysis of triacylglycerols (TAG) with a 1,3‐specific lipase is described. After partial lipase hydrolysis of the triacylglycerol, the released free fatty acids (FFA) and 1,2(2,3)‐diacylglycerols (DAG) were isolated by thin‐layer chromatography (TLC) and converted to fatty acid methyl esters (FAME ...
Thomas A. Foglia, Edith J. Conkerton, Philip E. Sonnet   +2 more
openaire   +1 more source

Comparison of the triacylglycerol hydrolase activity of human post-heparin plasma lipoprotein lipase and hepatic triacylglycerol lipase. A monolayer study

Biochemistry, 1986
Interfacial catalysis of hepatic triacylglycerol lipase (H-TGL) and lipoprotein lipase (LpL) isolated from human post-heparin plasma was investigated with mixed monolayers of trioleoylglycerol (TO) and egg phosphatidylcholine. Rates of enzyme catalysis were dependent on surface pressure, substrate concentration, apoC-II (the activator protein for LpL),
R L, Jackson, E, Ponce, L R, McLean, R A, Demel   +3 more
openaire   +2 more sources

Use of a fluorescent radiolabeled triacylglycerol as a substrate for lipoprotein lipase and hepatic triglyceride lipase

Lipids, 1988
AbstractA fluorescent radiolabeled triacylglycerol has been synthesized by using a fluorescent fatty acid (pyrene decanoic acid) and a radiolabeled oleic acid. This analog of the natural substrate, 1(3)pyrene decanoic‐2,3(1,2)‐dioleoyl‐sn‐glycerol, has been tested as substrate for determining lipoprotein lipase and hepatic triacylglycerol lipase ...
N, Dousset, A, Negre, R, Salvayre, P, Rogalle, Q Q, Dang, L, Douste-Blazy   +5 more
openaire   +2 more sources

Characterization of triacylglycerol lipase activity in human amniotic fluid

American Journal of Obstetrics and Gynecology, 1980
A radiochemical assay was used to measure the triacylglycerol lipase activity found in normal human amniotic fluid at term. Enzyme activity was characterized in a partially purified extract of amniotic fluid and was found to be optimal at pH 8.0 +/- 0.2 in the presence of 5mM sodium taurocholate, with the use of emulsified tri-[3H]oleoyl glycerol as ...
C, Merger, A, Valette, H, Ruf, J, Boyer
openaire   +2 more sources

Immunochemical assay of rat postheparin plasma triacylglycerol lipases

Atherosclerosis, 1980
Immunochemical methods for selective measurement of lipoprotein lipase and hepatic lipase activities in rat postheparin plasma are described and validated. Lipoprotein lipase was measured using a substrate containing 10% serum and 0.1 M NaCl after inactivation of hepatic lipase with a specific antiserum. Hepatic lipase was measured at 1.0 M NaCl with a
T, Kuusi, C, Ehnholm, E A, Nikkilä
openaire   +2 more sources

A spectrophotometric assay for lipase activity utilizing immobilized triacylglycerols

Journal of Biochemical and Biophysical Methods, 1991
New substrates for the determination of lipase activity have been developed. Triacylglycerols were immobilized by adsorption on an appropriate carrier or adsorbent yielding a lipase substrate in a powder form. The adsorbed triacylglycerols were easily hydrolyzed by lipases present in a reaction mixture.
openaire   +2 more sources

Homology modeling of lipoprotein lipase and hepatic triacylglycerol lipase

Clinical Biochemistry, 2011
Faezeh Hosseinzade, Nusha Soheyli Azad
openaire   +1 more source

[Fish proteins moderate triacylglycerols, activities of hepatic triacylglycerol lipase and tissue lipoprotein lipases in hypertensive and diabetic rats].

Archives des maladies du coeur et des vaisseaux, 2006
Fish proteins effect compared with casein was determined on triacylglycerols (TG) metabolism and activities of hepatic triacylglycerol lipase (HTGL) and tissue lipoprotein lipases (LPL), in SHR and diabetic SHR. Two groups of rats (n=24) were fed, 2 months, diet containing 20% casein (CAS) or 20% fish proteins (FP). One month before sacrifice, diabetes
F, Ouda Boukortt, A, Girard, J, Prost, J, Belleville, M, Bouchenak   +4 more
openaire   +1 more source

Triacylglycerol lipase

1991
Dietmar Schomburg, Margit Salzmann
openaire   +1 more source

Bacterial triacylglycerol lipase is a potential cholesterol esterase: Identification of a key determinant for sterol-binding specificity

International Journal of Biological Macromolecules, 2021
Yoshiaki Yasutake, Sachiyo Aburatani, Tomohiro Tamura   +2 more
exaly