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Cellulase production by Trichoderma reesei
European Journal of Applied Microbiology and Biotechnology, 1983A model is proposed for the enzyme production by Trichoderma reesei (QM 9414), which assumes control of the active enzyme transport through the cell membrane as a key parameter for the enzyme activity change in the culture filtrate. In a stirred tank reactor, continuous cultivation of the fungus was carried out in the dilution rate range of D=0.01–0 ...
Anton Ross +2 more
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Ecological Genomics and Evolution of Trichoderma reesei
2020The filamentous fungus Trichoderma reesei (Hypocreales, Ascomycota) is an efficient industrial cell factory for the production of cellulolytic enzymes used for biofuel and other applications. Therefore, researches addressing T. reesei are relatively advanced compared to other Trichoderma spp.
Mohammad Javad Rahimi +7 more
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Electrofusion of Trichoderma reesei protoplasts
Letters in Applied Microbiology, 1987Protoplasts of Trichoderma reesei were fused according to the method of Zimmermann. For optimizing the fusion parameters the central composite design was used. Genetic evidence for fusion has been obtained by segregation of the auxotrophic markers in the haploid conidia. The parameters which were optimized were: pulse voltage, pulse duration and number
Walter Steiner +2 more
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Batch Cultivation of Trichoderma reesei
2020This chapter explains how to perform a batch cultivation of Trichoderma reesei in bench top bioreactors, exemplarily using wheat straw as sole carbon source, and a selection of recommended, frequently used analyses to monitor the cultivation (intra- and extracellular as well), which are microscopic analysis, sodium hydroxide soluble protein, Bradford ...
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Characterization of the Trichoderma reesei cbh2 promoter
Current Genetics, 1993A 613-bp fragment of the 5' upstream region of the Trichoderma reesei cbh2 gene (coding for the cellulolytic enzyme cellobiohydrolase II) has been isolated and sequenced. Fusion of this fragment to the E. coli uidA gene (coding for beta-glucuronidase) leads to--albeit low--expression of beta-glucuronidase activity in the presence of cellulose and upon ...
Franz Gruber +2 more
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RNA Characterization in Trichoderma reesei
2020This chapter provides an overview on different methods for the characterization of RNAs in Trichoderma reesei. In the first section, protocols for the extraction of total RNA from fungal mycelia and the identification of 5' and 3' ends of certain RNAs of interest via rapid amplification of cDNA ends (RACE) are presented.
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Proteomic Profiling of the Secretome of Trichoderma reesei
2020Trichoderma reesei (T. reesei) is the workhorse for the production of industrial cellulolytic enzyme cocktails for cellulose hydrolysis. However, the current industrial process using enzyme cocktails is not efficient enough for the cost-effective generation of cellulosic sugar.
Siu Kwan Sze, So Fong Cam Ngan
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Trends in Biotechnology, 1983
Abstract Recent advances in the delineation of the biochemical mechanisms of cellulose hydrolysis, strain improvement, molecular cloning and process engineering are bringing T. reesei cellulases closer to being a commercially viable route to cellulose hydrolysis.
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Abstract Recent advances in the delineation of the biochemical mechanisms of cellulose hydrolysis, strain improvement, molecular cloning and process engineering are bringing T. reesei cellulases closer to being a commercially viable route to cellulose hydrolysis.
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Chitinous Material in Trichoderma reesei
Biotechnology Letters, 1998Trichoderma reesei was grown for 180h in batch culture in an 8 liter stirred fermenter using a glucose-rich medium. Concentrations of glucose, ammonia, cell dry weight, debris and lipid are presented for two runs. Cell dry weights reached 26.9g/L and 19.6g/L in these runs.
A. J. Thornton, D. E. Brown
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Kinetics of protein secretion in Trichoderma reesei
1996In order to obtain knowledge of the protein secretion process in filamentous fungi, and to study the rate-limiting steps in protein production, we have developed methodology for measuring the kinetics of protein production and secretion by metabolic labelling in Trichoderma.
Merja Penttilä +5 more
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