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Subunit Interactions and Catalytic Activity of Triose Phosphate Isomerase
Enzyme, 1982Rabbit and chicken triose phosphate isomerase were labelled with iodo-(1-14C)-acetamide. The efficiencies of renaturation after denaturation in guanidinium chloride were similar to those of the unlabelled enzymes. The enzymes were immobilised to Sepharose 4B and after guanidinium treatment only immobilised monomers remained on the gel.
C J, Branford White, D A, Fell
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Atomic coordinates for triose phosphate isomerase from chicken muscle
Biochemical and Biophysical Research Communications, 1976Abstract Atomic coordinates are presented for the 3740 atoms other than hydrogen in the dimeric molecule of chicken muscle triose phosphate isomerase. They are derived from an electron-density map at 2.5Aresolution, interpreted in terms of the known amino-acid sequence, and they have been adjusted systematically to give stereochemically appropriate ...
D W, Banner +4 more
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Equilibrium Unfolding Mechanism of Chicken Muscle Triose Phosphate Isomerase
Protein & Peptide Letters, 2008Triose phosphate isomerase (TIM) was prepared and purified from chicken breast muscle. The equilibrium unfolding of TIM by urea was investigated by following the changes of intrinsic fluorescence and circular dichroism spectroscopy, and the equilibrium thermal unfolding by differential scanning calorimetry (DSC).
Yi, Shi +3 more
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Recent amplification of triose phosphate isomerase related sequences in lettuce
Genome, 1992A random cDNA clone was identified as distinguishing near-isogenic lines for downy mildew resistance in lettuce. The clone detected multiple restriction fragments in genomic Southern blots of lettuce. Restriction fragment length polymorphisms (RFLPs) detected by this clone mapped to separate clusters of resistance genes; therefore, these sequences ...
I, Paran +3 more
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Studies on liver and muscle triose phosphate isomerase
Archives of Biochemistry and Biophysics, 1966Abstract Liver triose phosphate isomerase was separated from liver alcohol dehydrogenase chromatographically. The liver and muscle isomerases were compared. Both isomerases were inhibited by glyceraldehyde 3-phosphate. This phenomenon was overcome by several stimulating agents.
Robert Snyder, Eun Woo Lee
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Metabolic Correction of Triose Phosphate Isomerase Deficiencyin Vitroby Complementation
Biochemical and Biophysical Research Communications, 1997Inherited deficiency of triose phosphate isomerase (TPI), the enzyme that catalyses the interconversion of dihydroxyacetone phosphate (DHAP) and glyceraldehyde-3-phosphate, is characterised by an accumulation of intracellular DHAP and markedly reduced enzyme activity in cells and tissues, resulting in progressive, usually fatal neuromuscular ...
A, Ationu +3 more
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Simulation analysis of triose phosphate isomerase: conformational transition and catalysis
Faraday Discussions, 1992A theoretical approach is employed to study the catalysis of the dihydroxyacetone phosphate (DHAP) to D-glyceraldehyde 3-phosphate (GAP) reaction by the enzyme triose phosphate isomerase (TIM). The conformational change in a loop involved in protecting the active site from solvent is examined by use of X-ray data and molecular dynamics simulations.
M, Karplus +4 more
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Phylogenetic utility of dynamin and triose phosphate isomerase
Systematic Entomology, 2007Abstract Dynamin and triose phosphate isomerase (Tpi), two markers suggested as potentially useful for insect phylogenetics, have been sequenced for 12 scale insect taxa (Hemiptera: Coccoidea). Protocols are given for their amplification using conventional polymerase chain reaction, and their phylogenetic ...
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Mitochondrial triose phosphate isomerase
Biochimica et Biophysica Acta, 1960G E, BOXER, C E, SHONK
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Chloroplast and cytoplasmic enzymes II. Pea leaf triose phosphate isomerases
Biochimica et Biophysica Acta (BBA) - Enzymology, 1971Abstract 1. 1. The predominant forms of pea (Pisum sativum) leaf chloroplast and cytoplasmic triose phosphate isomerases ( d -glyceraldehyde 3-phosphate ketol-isomerase, EC 5.3.1.1), separated by isoelectric focusing, have been characterized. 2. 2.
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