Results 41 to 50 of about 216,465 (259)

Reconstructing enzyme evolution by protein engineering

FEBS Letters, EarlyView.
Natural enzyme evolution can be retraced by protein engineering methods such as directed evolution, rational design, and ancestral sequence reconstruction. These approaches reveal how enzymes emerged from ligand‐binding scaffolds, developed varying substrate preferences, formed oligomeric complexes, adapted to environmental changes, and evolved novel ...
Lukas Drexler, Markus R. Busch, Reinhard Sterner   +2 more
wiley   +1 more source

Engineering peptides into antibodies—opportunities and strategies for therapeutic innovation

FEBS Letters, EarlyView.
Peptides and antibodies occupy complementary therapeutic niches. Peptides recognize difficult targets in a compact format, while antibodies add specificity, long half‐life, and effector functions. This review examines strategies that merge both modalities—peptide grafting into loops, terminal and Fc fusions, and bioconjugation—highlighting how ...
Jinling Wang, Ruslana Romaniuk, Hristo L. Svilenov   +2 more
wiley   +1 more source

The role of single inoculation and dubie and triple bacterial interaction Azospirullum brasilanse and VAM Glomuse Mossea and phosphate rock in availability NPK In the Rhizosphere Cucumber ( cucmuise stivease L. ) [PDF]

Al-Qadisiyah Journal For Agriculture Sciences, 2019
Use biofirtilizer forming for quantity Bacterial Azospirullum braslieanse and VAM Glomus  mossea  and  four  levels of   rock  phosphate ( 0 , 80, 140 , 200 Kg  . ha -1) to study the single effect and double and triple interaction in the availability NPK 
Hatem N. Arabi, Jawad A. Kamal
doaj   +2 more sources

Structural and biochemical analysis of a B12 superbinder

FEBS Letters, EarlyView.
BtuG proteins are vitamin B12 scavengers in Bacteroides thetaiotaomicron, a dominant human gut bacterium. We present crystal structures of three BtuG homologs bound to cobalamin and its precursor cobinamide, revealing picomolar binding affinities, among the highest known for any natural protein.
Jose M. Martinez Felices, Jacob J. Whittaker, Dirk J. Slotboom, Albert Guskov   +3 more
wiley   +1 more source

Study of Statistical Convergence of Triple Sequences in a Topological Space

Revista Ingeniería, Matemáticas y Ciencias de la Información
In this paper, we introduce statistical convergence of triple sequences which are defined in a topological space. Various results on statistically convergent triple sequences are produced by using the notion of triple natural density operator.
Birojit Das, Apurba Debnath, Carlos Granados, Abdullah Aydin   +3 more
doaj   +1 more source

Comprendre l’interprétation d’un corpus

Théorème, 2023
The term of « sequence-shot » has been coined by André Bazin, and nowadays refers to a well-known concept. However this concept originates both in the context of post-war France and in the necessity to understand a particular tendency of American films –
Laurent Le Forestier
doaj   +1 more source

Structures of mycobacterial 3‐methylcrotonyl‐CoA carboxylase reveal carrier‐domain translocation between catalytic sites

FEBS Letters, EarlyView.
Mycobacterial 3‐methylcrotonyl‐CoA carboxylase uses a mobile biotin‐carrying domain to shuttle a carboxyl group between two catalytic sites, enabling carboxylation of 3‐methylcrotonyl‐CoA during leucine breakdown. Cryo‐electron microscopy captures the carrier at both sites and reveals an inward loop movement that may prevent futile rebinding to the ...
Ajit Yadav, Bogdan I. Florea, Sebastian Geibel   +2 more
wiley   +1 more source

The Shewanella oneidensis Fic enzyme SoFic targets the switch‐I region of EF‐Tu for AMPylation

FEBS Letters, EarlyView.
Fic enzymes mediate diverse post‐translational modifications across all domains of life, including AMPylation. Prokaryotic EF‐Tu can be AMPylated and deAMPylated by the conserved Fic enzyme SoFic. Structural and biochemical approaches were used to characterize the effect of AMPylation on EF‐Tu, SoFic's enzymatic activities, and the enzyme‐target ...
Svenja Runge, Vivian Pogenberg, Alexander Baumgart, Bente Siebels, Hartmut Schlüter, Michael Hecht‐Bucher, Aymelt Itzen   +6 more
wiley   +1 more source

Prospecting the protein design landscape

FEBS Letters, EarlyView.
This review outlines the current state of various protein design approaches. We discuss the current possibilities enabled by recently released tools, highlight future avenues to pursue in protein design, and underscore the crucial role of key databases and resources for successful protein design workflows.
Jakob R. Riccabona, Katharina T. Stonig, Jens Meiler, Clara T. Schoeder, Monica L. Fernández‐Quintero   +4 more
wiley   +1 more source

Characterization of an Escherichia coli-derived triple-type chimeric vaccine against human papillomavirus types 39, 68 and 70

npj Vaccines, 2022
In vaccinology, a potent immunogen has two prerequisite attributes—antigenicity and immunogenicity. We have rational designed a triple-type HPV vaccine against HPV58, −33 and −52 covered in Gardasil 9 based on the sequence homology and similar surface ...
Ciying Qian, Yurou Yang, Qin Xu, Zhiping Wang, Jie Chen, Xin Chi, Miao Yu, Fei Gao, Yujie Xu, Yihan Lu, Hui Sun, Jingjia Shen, Daning Wang, Lizhi Zhou, Tingting Li, Yingbin Wang, Qingbing Zheng, Hai Yu, Jun Zhang, Ying Gu, Ningshao Xia, Shaowei Li   +21 more
doaj   +1 more source