Results 11 to 20 of about 447,474 (316)
Post-TRIzol protein extraction from peripheral blood mononuclear cells [PDF]
Journal of the Serbian Chemical Society, 2023 After sample processing for RNA and DNA analysis, the leftover protein pellets are usually discarded due to the limited efficiency of pellet reconstitution/solubilisation. As the pelleted proteins are tightly packed, they are most often solubilised using
Stevanović Jovana +3 more
doaj +3 more sources
Modification of the Trizol Method for the Extraction of RNA from Prorocentrum triestinum ACIZ_LEM2. [PDF]
Int J Mol SciIn samples of harmful algal blooms (HABs), seawater can contain a high abundance of microorganisms and elemental ions. Along with the hardness of the walls of key HAB dinoflagellates such as Prorocentrum triestinum, this makes RNA extraction very difficult.
Huarachi-Olivera R +10 more
europepmc +4 more sources
DNA Extraction with TRIzol Reagent Using a Silica Column
Analytical Sciences, 2020 TRIzol is a monophasic solution of phenol and guanidine isothiocyanate used for the extraction of RNA, DNA and proteins from tissues or cells. However, few studies have described its application to DNA extraction due to its time-consuming procedure.
Yang Bohan, Baoshan Liu, Zeliang Chen
semanticscholar +6 more sources
TRIzol and Alu qPCR-based quantification of metastatic seeding within the skeleton [PDF]
Scientific Reports, 2015 Current methods for detecting disseminated tumor cells in the skeleton are limited by expense and technical complexity. We describe a simple and inexpensive method to quantify, with single cell sensitivity, human metastatic cancer in the mouse skeleton ...
James Campbell +8 more
semanticscholar +5 more sources
RNA extraction protocol (Trizol) v1 [PDF]
, 2016 This protocol describes how to extract total RNA from flatworms. It is from: Hebert, F, O; Grambauer, S; Barber, I; Landry, C, R; Aubin-Horth, N (2016): Reference transcriptome sequence resource for the study of the Cestode Schistocephalus solidus, a threespine stickleback parasite. GigaScience Database. http://dx.doi.org/10.5524/100197
Hebert F.O. +4 more
semanticscholar +4 more sources
Evaluation of TRIzol LS Inactivation of Viruses [PDF]
Applied Biosafety, 2017 Prior to the release from BSL-3 or -4 laboratories, materials from which nucleic acids are to be extracted must be rendered noninfectious.
Tadeusz J. Kochel +3 more
semanticscholar +4 more sources
A TRIzol-based method for high recovery of plasma sncRNAs approximately 30 to 60 nucleotides [PDF]
Scientific Reports, 2022 Protein functional effector sncRNAs (pfeRNAs) are approximately 30–60 nucleotides (nt), of which the extraction method from plasma has not yet been reported.
Kristen Rodgers +6 more
semanticscholar +5 more sources
Strategies for Validation of Inactivation of Viruses with Trizol® LS and Formalin Solutions
Applied Biosafety, 2020 Introduction: Inactivation of biological agents and particularly select agents has come under increased scrutiny since the US Army inadvertently shipped live anthrax both inside and outside the US, leading to more stringent regulations regarding ...
Cary Retterer +8 more
semanticscholar +6 more sources
An optimized TRIzol-based method for isolating RNA from adipose tissue.
BioTechniques, 2023 High-quality RNA isolation from recalcitrant adipose tissue with high lipid content and low cell numbers is difficult. Many studies have made efforts to optimize methods for isolating RNA from adipose tissue through combinations of column-based kits and ...
Hongwei Zhang +3 more
semanticscholar +4 more sources
Proteome Science, 2020 Background Marine medaka is among the most popular models of fish species for ecotoxicology and environmental research and proteomic studies are useful tools for understanding the molecular responses of medaka upon exposure to different environmental ...
Celia Sze-Nga Kwok +5 more
doaj +3 more sources