Results 11 to 20 of about 461,258 (325)
An Optimized TRIzol-Based Method for Isolating RNA from Adipose Tissue
BioTechniques, 2023 High-quality RNA isolation from recalcitrant adipose tissue with high lipid content and low cell numbers is difficult. Many studies have made efforts to optimize methods for isolating RNA from adipose tissue through combinations of column-based kits and phenol-chloroform methods, or through in-house protocols.
Hongwei Zhang +3 more
semanticscholar +5 more sources
DNA Extraction with TRIzol Reagent Using a Silica Column
Analytical Sciences, 2020 TRIzol is a monophasic solution of phenol and guanidine isothiocyanate used for the extraction of RNA, DNA and proteins from tissues or cells. However, few studies have described its application to DNA extraction due to its time-consuming procedure. We present a TRIzol-modified method of extracting DNA from tissues using the TRIzol reagent and a silica
Yang Bohan, Baoshan Liu, Zeliang Chen
semanticscholar +5 more sources
Strategies for Validation of Inactivation of Viruses with Trizol® LS and Formalin Solutions
Applied Biosafety, 2020 Inactivation of biological agents and particularly select agents has come under increased scrutiny since the US Army inadvertently shipped live anthrax both inside and outside the US, leading to more stringent regulations regarding inactivation.Formalin and Trizol® LS were used to inactivate virus samples in complex matrices.
Cary Retterer +8 more
semanticscholar +5 more sources
RNA extraction using the 'home-made' Trizol substitute v1
, 2016 Asimple protocol for RNA extraction from various types of samples (protists, fungi, bacteria, organelles, subcellular fractions, ribosomes, etc.). Compared tothe commercial Trizol® procedure, it makes use ofgenerally available chemicals without the need for columns,thusallowing concurrent extraction oftranscripts of all sizes (including RNAs <200 nt)
Matus Valach
semanticscholar +3 more sources
Proteome Science, 2020 Background Marine medaka is among the most popular models of fish species for ecotoxicology and environmental research and proteomic studies are useful tools for understanding the molecular responses of medaka upon exposure to different environmental ...
Celia Sze-Nga Kwok +5 more
doaj +3 more sources
PLoS ONE, 2021 The use of saliva for the diagnosis of SARS-CoV-2 has shown to be a good alternative to nasopharyngeal swabs (NPS), since it permits self-collection, avoids the exposure of healthy persons to infected patients, reduces waiting times, eliminates the need ...
Haydee Miranda-Ortiz +15 more
doaj +2 more sources
High-purity DNA extraction from animal tissue using picking in the TRIzol-based method
BioTechniques, 2021 TRIzol is used for the extraction of RNA, DNA and proteins from tissues or cells. Here, we present a simple picking method to extract DNA from tissues using TRIzol.
Ye Yin Bo +6 more
doaj +2 more sources
Post-TRIzol protein extraction from peripheral blood mononuclear cells [PDF]
Journal of the Serbian Chemical Society, 2023 After sample processing for RNA and DNA analysis, the leftover protein pellets are usually discarded due to the limited efficiency of pellet reconstitution/solubilisation. As the pelleted proteins are tightly packed, they are most often solubilised using
Stevanović Jovana +3 more
doaj +2 more sources
Evaluation of TRIzol LS Inactivation of Viruses [PDF]
Applied Biosafety, 2017 Prior to the release from BSL-3 or -4 laboratories, materials from which nucleic acids are to be extracted must be rendered noninfectious.
Tadeusz J. Kochel +3 more
openaire +2 more sources
High-yield skeletal muscle protein recovery from TRIzol after RNA and DNA extraction
BioTechniques, 2020 Extraction of DNA, RNA and protein from the same sample would allow for direct comparison of genomic, transcriptomic and proteomic information. Commercially available kits exhibit poor protein yield and the TRIzol® reagent produces a protein pellet that ...
Yuan Wen +3 more
doaj +2 more sources