Results 11 to 20 of about 452,059 (305)

Modification of the Trizol Method for the Extraction of RNA from Prorocentrum triestinum ACIZ_LEM2 [PDF]

International Journal of Molecular Sciences
In samples of harmful algal blooms (HABs), seawater can contain a high abundance of microorganisms and elemental ions. Along with the hardness of the walls of key HAB dinoflagellates such as Prorocentrum triestinum, this makes RNA extraction very difficult.
Ronald Huarachi-Olivera, María Teresa Mata, Alonso Ardiles-Candia, Valentina Escobar-Méndez, Carlos Gatica-Cortes, M. Isabel Ahumada, José Orrego, Boris Vidal‐Veuthey, Juan Pablo Cárdenas, Leonel González, Carlos Riquelme   +10 more
europepmc   +4 more sources

RNA extraction protocol (Trizol) v1 [PDF]

, 2016
This protocol describes how to extract total RNA from flatworms. It is from: Hebert, F, O; Grambauer, S; Barber, I; Landry, C, R; Aubin-Horth, N (2016): Reference transcriptome sequence resource for the study of the Cestode Schistocephalus solidus, a threespine stickleback parasite. GigaScience Database. http://dx.doi.org/10.5524/100197
Hebert F.O., not provided Grambauer S., not provided Barber I., not provided Landry C.R., not provided Aubin-Horth N.   +4 more
openalex   +3 more sources

Evaluation of TRIzol LS Inactivation of Viruses [PDF]

Applied Biosafety, 2017
Prior to the release from BSL-3 or -4 laboratories, materials from which nucleic acids are to be extracted must be rendered noninfectious.
Tadeusz J. Kochel, Gregory Kocher, Thomas G. Ksiazek, James P. Burans   +3 more
openalex   +3 more sources

Proteome profiling of extracellular vesicles captured with the affinity peptide Vn96: comparison of Laemmli and TRIzol© protein-extraction methods

Journal of Extracellular Vesicles, 2018
Sample amount is often a limiting factor for multi-parametric analyses that encompass at least three areas of ‘-omics’ research: genomics, transcriptomics and proteomics.
Andrew P. Joy, D. Craig Ayre, Ian C. Chute, Annie-Pier Beauregard, Gabriel Wajnberg, Anirban Ghosh, Stephen M. Lewis, Rodney J. Ouellette, David A. Barnett   +8 more
doaj   +2 more sources

DNA Extraction with TRIzol Reagent Using a Silica Column

Analytical Sciences, 2020
TRIzol is a monophasic solution of phenol and guanidine isothiocyanate used for the extraction of RNA, DNA and proteins from tissues or cells. However, few studies have described its application to DNA extraction due to its time-consuming procedure. We present a TRIzol-modified method of extracting DNA from tissues using the TRIzol reagent and a silica
Yang Bohan, Baoshan Liu, Zeliang Chen
openalex   +5 more sources

TRIzol treatment of secretory phase endometrium allows combined proteomic and mRNA microarray analysis of the same sample in women with and without endometriosis [PDF]

Reproductive Biology and Endocrinology, 2010
Background According to mRNA microarray, proteomics and other studies, biological abnormalities of eutopic endometrium (EM) are involved in the pathogenesis of endometriosis, but the relationship between mRNA and protein expression in EM is not clear. We
Gevaert Olivier, Meuleman Christel, Mihalyi Attila, Waelkens Etienne, Kyama Cleophas M, Simsa Peter, Fassbender Amelie, Van de Plas Raf, de Moor Bart, D'Hooghe Thomas M   +9 more
doaj   +2 more sources

Modified CTAB and TRIzol protocols improve RNA extraction from chemically complex Embryophyta [PDF]

Applications in Plant Sciences, 2015
Premise of the study:Here we present a series of protocols for RNA extraction across a diverse array of plants; we focus on woody, aromatic, aquatic, and other chemically complex taxa.Methods and Results:Ninety‐one taxa were subjected to RNA extraction with three methods presented here: (1) TRIzol/TURBO DNA‐free kits using the manufacturer's protocol ...
Ingrid Jordon‐Thaden, André S. Chanderbali, Matthew A. Gitzendanner, Pamela S. Soltis   +3 more
openalex   +5 more sources

Optimizing total RNA extraction method for human and mice samples [PDF]

PeerJ
Background Extracting high-quality total RNA is pivotal for advanced RNA molecular studies, such as Next-generation sequencing and expression microarrays where RNA is hybridized.
Yumei Zeng, Xiaoxue Tang, Jinwen Chen, Xi Kang, Dazhang Bai   +4 more
doaj   +3 more sources

Strategies for Validation of Inactivation of Viruses with Trizol® LS and Formalin Solutions

Applied Biosafety, 2020
Inactivation of biological agents and particularly select agents has come under increased scrutiny since the US Army inadvertently shipped live anthrax both inside and outside the US, leading to more stringent regulations regarding inactivation.Formalin and Trizol® LS were used to inactivate virus samples in complex matrices.
Cary Retterer, Tara Kenny, Rouzbeh Zamani, Louis A. Altamura, Brian Kearney, Jim Jaissle, S. R. Coyne, Scott P. Olschner, David E. Harbourt   +8 more
openalex   +5 more sources

A TRIzol-based method for high recovery of plasma sncRNAs approximately 30 to 60 nucleotides [PDF]

Scientific Reports, 2022
AbstractProtein functional effector sncRNAs (pfeRNAs) are approximately 30–60 nucleotides (nt), of which the extraction method from plasma has not yet been reported. Silver staining in a high-resolution polyacrylamide gel suggested that the majority of plasma sncRNAs extracted by some broadly used commercial kits were sncRNAs from 100 nt upwards ...
Kristen Rodgers, Alicia Hulbert, Hamza Khan, Maria Shishikura, Shun Ishiyama, Malcolm V. Brock, Yuping Mei   +6 more
openalex   +4 more sources