Results 11 to 20 of about 463,109 (314)
TRIzol treatment of secretory phase endometrium allows combined proteomic and mRNA microarray analysis of the same sample in women with and without endometriosis [PDF]
Reproductive Biology and Endocrinology, 2010 Background According to mRNA microarray, proteomics and other studies, biological abnormalities of eutopic endometrium (EM) are involved in the pathogenesis of endometriosis, but the relationship between mRNA and protein expression in EM is not clear. We
Gevaert Olivier +9 more
doaj +2 more sources
Post-TRIzol protein extraction from peripheral blood mononuclear cells [PDF]
Journal of the Serbian Chemical Society, 2023 After sample processing for RNA and DNA analysis, the leftover protein pellets are usually discarded due to the limited efficiency of pellet reconstitution/solubilisation. As the pelleted proteins are tightly packed, they are most often solubilised using
Stevanović Jovana +3 more
doaj +2 more sources
DNA Extraction with TRIzol Reagent Using a Silica Column
Analytical Sciences, 2020 TRIzol is a monophasic solution of phenol and guanidine isothiocyanate used for the extraction of RNA, DNA and proteins from tissues or cells. However, few studies have described its application to DNA extraction due to its time-consuming procedure. We present a TRIzol-modified method of extracting DNA from tissues using the TRIzol reagent and a silica
Bo-Han, Yang +2 more
openaire +4 more sources
RNA Extraction from Nematodes Using Trizol v1 [PDF]
This protocol describes whole RNA extraction from nematode bulk samples using Trizol. The RNA is then treated with DNase to remove any remaining DNA. Quality control usually shows a RIN value of 8 or higher. Depending on the input you get several micrograms of RNA.
Manuela R. Kieninger
openaire +2 more sources
Coral tissue and skeleton Trizol RNA extraction v1 [PDF]
, 2020 This protocol was modified from the TRIzol protocol provided by Invitrogen. https://assets.thermofisher.com/TFS-Assets/LSG/manuals/trizol_reagent.pdf
Molly A. Moynihan
openaire +2 more sources
Optimizing total RNA extraction method for human and mice samples [PDF]
PeerJBackground Extracting high-quality total RNA is pivotal for advanced RNA molecular studies, such as Next-generation sequencing and expression microarrays where RNA is hybridized.
Yumei Zeng +4 more
doaj +3 more sources
RNA extraction using the 'home-made' Trizol substitute v1
, 2016 Asimple protocol for RNA extraction from various types of samples (protists, fungi, bacteria, organelles, subcellular fractions, ribosomes, etc.). Compared tothe commercial Trizol® procedure, it makes use ofgenerally available chemicals without the need for columns,thusallowing concurrent extraction oftranscripts of all sizes (including RNAs <200 nt)
Matus Valach
openaire +2 more sources
An Optimized TRIzol-Based Method for Isolating RNA from Adipose Tissue
BioTechniques, 2023 High-quality RNA isolation from recalcitrant adipose tissue with high lipid content and low cell numbers is difficult. Many studies have made efforts to optimize methods for isolating RNA from adipose tissue through combinations of column-based kits and phenol-chloroform methods, or through in-house protocols.
Hongwei Zhang +3 more
openaire +4 more sources
TRIzol-based RNA Extraction: A Reliable Method for Gene Expression Studies [PDF]
Journal of Sciences, Islamic Republic of Iran, 2014 RNA extraction is a prerequisite technique for gene expression studies, analyzing the etiology and disease progression, treatment effects, as well as designing the diagnostic methods.
M. Pahlevan Kakhki
doaj +2 more sources
RNA Extraction from Drosophila Tissues using TRIzol Reagent v2
, 2016 This protocol is adapted from the Invitrogen Life Technologies Trizol manual.
Elizabeth Allen
openaire +2 more sources