Optimized microRNA purification from TRIzol-treated plasma [PDF]
BMC Genomics, 2015 MicroRNAs (miRNAs) represent new and potentially informative diagnostic targets for disease detection and prognosis. However, little work exists documenting the effect of TRIzol, a common viral inactivation and nucleic acid extraction reagent, on miRNA purification.
Duy, Janice +3 more
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TRIzol treatment of secretory phase endometrium allows combined proteomic and mRNA microarray analysis of the same sample in women with and without endometriosis [PDF]
Reproductive Biology and Endocrinology, 2010 Background According to mRNA microarray, proteomics and other studies, biological abnormalities of eutopic endometrium (EM) are involved in the pathogenesis of endometriosis, but the relationship between mRNA and protein expression in EM is not clear. We
Gevaert Olivier +9 more
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Optimizing total RNA extraction method for human and mice samples [PDF]
PeerJBackground Extracting high-quality total RNA is pivotal for advanced RNA molecular studies, such as Next-generation sequencing and expression microarrays where RNA is hybridized.
Yumei Zeng +4 more
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Modification of the Trizol Method for the Extraction of RNA from Prorocentrum triestinum ACIZ_LEM2
International Journal of Molecular SciencesIn samples of harmful algal blooms (HABs), seawater can contain a high abundance of microorganisms and elemental ions. Along with the hardness of the walls of key HAB dinoflagellates such as Prorocentrum triestinum, this makes RNA extraction very difficult.
Ronald Huarachi-Olivera +10 more
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TRIzol and Alu qPCR-based quantification of metastatic seeding within the skeleton [PDF]
Scientific Reports, 2015 AbstractCurrent methods for detecting disseminated tumor cells in the skeleton are limited by expense and technical complexity. We describe a simple and inexpensive method to quantify, with single cell sensitivity, human metastatic cancer in the mouse skeleton, concurrently with host gene expression, using TRIzol-based DNA/RNA extraction and Alu ...
J. Preston Campbell +8 more
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Genomic RNA extraction by TRIzol method by using Drosophila melanogastergut v1 [PDF]
, 2022 Simple method followed for RNA extraction of Drosophila melanogaster larval(3rd instar) gut for CDNA
Mohamed Sabeelil Islam
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Coral tissue and skeleton Trizol RNA extraction v1 [PDF]
, 2020 This protocol was modified from the TRIzol protocol provided by Invitrogen. https://assets.thermofisher.com/TFS-Assets/LSG/manuals/trizol_reagent.pdf
Molly A. Moynihan
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RNA Extraction from Nematodes Using Trizol v1 [PDF]
This protocol describes whole RNA extraction from nematode bulk samples using Trizol. The RNA is then treated with DNase to remove any remaining DNA. Quality control usually shows a RIN value of 8 or higher. Depending on the input you get several micrograms of RNA.
Manuela R. Kieninger
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Automated RNA Purification directly from TRIzol® on the Hamilton Microlab® STAR™ [PDF]
BioTechniques, 2018 The TRIzol method for RNA extraction has been the gold standard. The powerful protein denaturant effectively stabilizes RNA and inactivates RNases and infectious agents.
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Biologia molecular aplicada às dermatoses tropicais Molecular biology in tropical dermatoses [PDF]
Anais Brasileiros de Dermatologia, 2008 São apresentados conceitos básicos sobre célula, código genético e síntese protéica, e sobre algumas técnicas de biologia molecular, tais como PCR, PCR-RFLP, seqüenciamento de DNA, RT-PCR e immunoblotting.
Ana Maria Roselino
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