Results 21 to 30 of about 452,059 (305)
Post-TRIzol protein extraction from peripheral blood mononuclear cells [PDF]
Journal of the Serbian Chemical Society, 2023 After sample processing for RNA and DNA analysis, the leftover protein pellets are usually discarded due to the limited efficiency of pellet reconstitution/solubilisation. As the pelleted proteins are tightly packed, they are most often solubilised using
Stevanović Jovana +3 more
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High-yield skeletal muscle protein recovery from TRIzol after RNA and DNA extraction
BioTechniques, 2020 Extraction of DNA, RNA and protein from the same sample would allow for direct comparison of genomic, transcriptomic and proteomic information. Commercially available kits exhibit poor protein yield and the TRIzol® reagent produces a protein pellet that ...
Yuan Wen +3 more
doaj +2 more sources
Effect of Long-term Storage in TRIzol on Microarray-Based Gene Expression Profiling [PDF]
Cancer Epidemiology, Biomarkers & Prevention, 2010 Abstract Background: Although TRIzol is widely used for preservation and isolation of RNA, there is suspicion that prolonged sample storage in TRIzol may affect array-based gene expression profiling (GEP) through premature termination during reverse transcription.
Wencai Ma +14 more
openalex +5 more sources
Virtual in situs: Sequencing mRNA from cryo-sliced Drosophila embryos to determine genome-wide spatial patterns of gene expression [PDF]
, 2013 Complex spatial and temporal patterns of gene expression underlie embryo differentiation, yet methods do not yet exist for the efficient genome-wide determination of spatial expression patterns during development.
Combs, Peter A., Eisen, Michael B.
core +19 more sources
Extraction RNA Protocol from Blood and Testis Tissue of Local Roosters
Tikrit Journal for Agricultural Sciences, 2021 The (one-step) method has become widely used to isolate total RNA from living organism samples and from different tissues. The aim of this study is to extract RNA from the blood and testicular tissue of male local chickens.
Ahmed Khalid +3 more
doaj +1 more source
Scientific Reports, 2022 The high quality, yield and purity total RNA samples are essential for molecular experiments. However, harvesting high quality RNA in Lilium davidii var.
Chunlei Wang +7 more
doaj +1 more source
Efficient recovery of proteins from multiple source samples after trizol® or trizol®LS RNA extraction and long-term storage [PDF]
BMC Genomics, 2013 Abstract Background Simultaneous isolation of nucleic acids and proteins from a single biological sample facilitates meaningful data interpretation and reduces time, cost and sampling errors. This is particularly relevant for rare human and animal specimens, often scarce, and/or irreplaceable.
Pedro M. Borralho +11 more
openaire +3 more sources
PLoS ONE, 2021 The use of saliva for the diagnosis of SARS-CoV-2 has shown to be a good alternative to nasopharyngeal swabs (NPS), since it permits self-collection, avoids the exposure of healthy persons to infected patients, reduces waiting times, eliminates the need ...
Haydee Miranda-Ortiz +15 more
doaj +1 more source
Proteome Science, 2020 Background Marine medaka is among the most popular models of fish species for ecotoxicology and environmental research and proteomic studies are useful tools for understanding the molecular responses of medaka upon exposure to different environmental ...
Celia Sze-Nga Kwok +5 more
doaj +1 more source