Results 21 to 30 of about 463,109 (314)
Automated RNA Purification directly from TRIzol® on the Hamilton Microlab® STAR™ [PDF]
BioTechniques, 2018 The TRIzol method for RNA extraction has been the gold standard. The powerful protein denaturant effectively stabilizes RNA and inactivates RNases and infectious agents.
doaj +2 more sources
Biologia molecular aplicada às dermatoses tropicais Molecular biology in tropical dermatoses [PDF]
Anais Brasileiros de Dermatologia, 2008 São apresentados conceitos básicos sobre célula, código genético e síntese protéica, e sobre algumas técnicas de biologia molecular, tais como PCR, PCR-RFLP, seqüenciamento de DNA, RT-PCR e immunoblotting.
Ana Maria Roselino
doaj +4 more sources
A TRIzol-Beadbeater RNA extraction method for yeast v1 [PDF]
Saccharomyces cerevisiae yeast cells were harvested by centrifugation and preserved in TRIzol reagent at −80 °C. Cell lysis was achieved via bead-beating in the presence of zirconium oxide beads using a high-speed homogenizer (Omni Bead Ruptor 12), resulting in >85% lysis after a single 30-second cycle, as verified by phase-contrast microscopy.
Sangeet Honey, Bruce Futcher
openaire +2 more sources
Extraction RNA Protocol from Blood and Testis Tissue of Local Roosters
Tikrit Journal for Agricultural Sciences, 2021 The (one-step) method has become widely used to isolate total RNA from living organism samples and from different tissues. The aim of this study is to extract RNA from the blood and testicular tissue of male local chickens.
Ahmed Khalid +3 more
doaj +1 more source
Scientific Reports, 2022 The high quality, yield and purity total RNA samples are essential for molecular experiments. However, harvesting high quality RNA in Lilium davidii var.
Chunlei Wang +7 more
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Optimized microRNA purification from TRIzol-treated plasma [PDF]
BMC Genomics, 2015 MicroRNAs (miRNAs) represent new and potentially informative diagnostic targets for disease detection and prognosis. However, little work exists documenting the effect of TRIzol, a common viral inactivation and nucleic acid extraction reagent, on miRNA purification.
Duy, Janice +3 more
openaire +2 more sources
High-purity DNA extraction from animal tissue using picking in the TRIzol-based method
BioTechniques, 2021 TRIzol is used for the extraction of RNA, DNA and proteins from tissues or cells. Here, we present a simple picking method to extract DNA from tissues using TRIzol.
Ye Yin Bo +6 more
doaj +1 more source
Virtual in situs: Sequencing mRNA from cryo-sliced Drosophila embryos to determine genome-wide spatial patterns of gene expression [PDF]
, 2013 Complex spatial and temporal patterns of gene expression underlie embryo differentiation, yet methods do not yet exist for the efficient genome-wide determination of spatial expression patterns during development.
Combs, Peter A., Eisen, Michael B.
core +12 more sources