Results 41 to 50 of about 452,059 (305)

COMPARISON OF RNA EXTRACTION METHODS FOR Passiflora edulis SIMS LEAVES

Revista Brasileira de Fruticultura, 2016
Functional genomic analyses require intact RNA; however, Passiflora edulis leaves are rich in secondary metabolites that interfere with RNA extraction primarily by promoting oxidative processes and by precipitating with nucleic acids. This study aimed to
ANNY CAROLYNE DA LUZ, IRANY RODRIGUES PRETTI, MARIA DO CARMO PIMENTEL BATITUCCI   +2 more
doaj   +1 more source

Evaluation of Different RNA Extraction Methods from Agropatch Suppressor Assay for Small Quantities of Plant Tissue and Their Application for Analysis of Gene Expression

Notulae Scientia Biologicae, 2018
The agroinfiltration assay provides fast and efficient way to transiently express genes into plant cells by Agrobacterium tumefaciens. Extraction of RNA of high quality and sufficient amounts is prerequisite for gene expression studies such as ...
Amir G. SHAHRIARI, Aminallah TAHMASEBI
doaj   +1 more source

Ciprofloxacin enhances the stimulation of matrix metalloproteinase 3 expression by interleukin-1beta in human tendon-derived cells [PDF]

, 2002
To determine whether the fluoroquinolone antibiotic ciprofloxacin, which can cause tendon pain and rupture in a proportion of treated patients, affects the expression of matrix metalloproteinases (MMPs) in human tendon-derived cells in culture.
Astrom, Bank, Bernard-Beaubois, Borden, Cawston, Clark, De Sarro, Ireland, Kato, Mauviel, Pierfitte, Rasmussen, Riesbeck, Riley, Shakibaei, Williams, Yoshimura   +16 more
core   +1 more source

An Optimized TRIzol-Based Method for Isolating RNA from Adipose Tissue

BioTechniques, 2023
High-quality RNA isolation from recalcitrant adipose tissue with high lipid content and low cell numbers is difficult. Many studies have made efforts to optimize methods for isolating RNA from adipose tissue through combinations of column-based kits and phenol-chloroform methods, or through in-house protocols.
Hongwei Zhang, Yaoming Liu, Bingcheng Yu, Rong Lu   +3 more
openaire   +3 more sources

Integrative analyses of transcriptome sequencing identify novel functional lncRNAs in esophageal squamous cell carcinoma. [PDF]

, 2017
Long non-coding RNAs (lncRNAs) have a critical role in cancer initiation and progression, and thus may mediate oncogenic or tumor suppressing effects, as well as be a new class of cancer therapeutic targets. We performed high-throughput sequencing of RNA
Ai, B, Bai, X-F, Cao, H-H, Feng, C-C, He, J-Z, Huang, G-W, Koeffler, HP, Lei, F, Li, C-Q, Li, E-M, Li, M, Li, S, Li, X, Li, X-C, Liao, L-D, Lin, D-C, Liu, Y-J, Shen, J-H, Tang, Z-D, Wang, L-D, Wang, M-R, Wang, Q-Y, Wang, S-H, Wu, B-L, Wu, J-Y, Wu, Z-Y, Xu, L-Y, Xu, Y-J, Xue, Y-J, Zhang, J, Zhao, J-M, Zheng, C-P, Zhu, Y   +32 more
core   +1 more source

High-Quality and High-Yield RNA Extraction Method From Whole Human Saliva

Biomarker Insights, 2020
Background: Human saliva has been identified as a novel, practical, and noninvasive source of biomarkers and genetic materials. However, it is equally challenging due to the availability of an abundance of impurities in the form of microbes and other ...
Vaibhav Gandhi, Mara H. O’Brien, Sumit Yadav   +2 more
doaj   +1 more source

Optimising total RNA quality and quantity by phenol-chloroform extraction method from human visceral adipose tissue: A standardisation study

MethodsX, 2020
A multitude of challenges is faced during RNA extraction from human visceral adipose tissue (VAT) due to its atypical nature and a dearth of existing literature.
Dipayan Roy, Sojit Tomo, Anupama Modi, Purvi Purohit, Praveen Sharma   +4 more
doaj   +1 more source

Stability investigation of air-dried olive ribo nucleic acids for metavirome studies

Plant Methods, 2022
Background The application of ribo nucleic acids for molecular studies requires high integrity and quality of extracted total RNA samples. In addition, the need to transfer RNA samples at room temperature without special treatments such as ice and liquid
Leila Mirzaei, Abbas Yadollahi, Maryam Jafarkhani Kermani, Masoud Naderpour, Ali Asghar Zeinanloo   +4 more
doaj   +1 more source

Rapid bedside inactivation of Ebola virus for safe nucleic acid tests [PDF]

, 2016
Rapid bedside inactivation of Ebola virus would be a solution for the safety of medical and technical staff, risk containment, sample transport and high-throughput or rapid diagnostic testing during an outbreak.
Bragstad, Karoline, Dudman, Susanne Gjeruldsen, Fomsgaard, Anders, Karlberg, Helen, Kran, Anne Marte Bakken, Lindegren, Gunnel, Mirazimi, Ali, Rosenstierne, Maiken Wors\uf8e, Salata, Cristiano, Stoltz, Malin Lundahl   +9 more
core   +2 more sources

MicroRNA regulation of type 2 innate lymphoid cell homeostasis and function in allergic inflammation. [PDF]

, 2017
MicroRNAs (miRNAs) exert powerful effects on immunity through coordinate regulation of multiple target genes in a wide variety of cells. Type 2 innate lymphoid cells (ILC2s) are tissue sentinel mediators of allergic inflammation.
Ansel, K Mark, Baumjohann, Dirk, Happ, Hannah C, Locksley, Richard M, Pua, Heather H, Schneider, Christoph, Singh, Priti B, von Moltke, Jakob   +7 more
core   +2 more sources