Results 41 to 50 of about 463,109 (314)

Evaluation of TRIzol LS Inactivation of Viruses [PDF]

Applied Biosafety, 2017
Prior to the release from BSL-3 or -4 laboratories, materials from which nucleic acids are to be extracted must be rendered noninfectious.
Tadeusz J. Kochel, Gregory A. Kocher, Thomas G. Ksiazek, James P. Burans   +3 more
openaire   +1 more source

Evaluation of Different RNA Extraction Methods from Agropatch Suppressor Assay for Small Quantities of Plant Tissue and Their Application for Analysis of Gene Expression

Notulae Scientia Biologicae, 2018
The agroinfiltration assay provides fast and efficient way to transiently express genes into plant cells by Agrobacterium tumefaciens. Extraction of RNA of high quality and sufficient amounts is prerequisite for gene expression studies such as ...
Amir G. SHAHRIARI, Aminallah TAHMASEBI
doaj   +1 more source

Rapid bedside inactivation of Ebola virus for safe nucleic acid tests [PDF]

, 2016
Rapid bedside inactivation of Ebola virus would be a solution for the safety of medical and technical staff, risk containment, sample transport and high-throughput or rapid diagnostic testing during an outbreak.
Bragstad, Karoline, Dudman, Susanne Gjeruldsen, Fomsgaard, Anders, Karlberg, Helen, Kran, Anne Marte Bakken, Lindegren, Gunnel, Mirazimi, Ali, Rosenstierne, Maiken Wors\uf8e, Salata, Cristiano, Stoltz, Malin Lundahl   +9 more
core   +2 more sources

Optimising total RNA quality and quantity by phenol-chloroform extraction method from human visceral adipose tissue: A standardisation study

MethodsX, 2020
A multitude of challenges is faced during RNA extraction from human visceral adipose tissue (VAT) due to its atypical nature and a dearth of existing literature.
Dipayan Roy, Sojit Tomo, Anupama Modi, Purvi Purohit, Praveen Sharma   +4 more
doaj   +1 more source

Integrative analyses of transcriptome sequencing identify novel functional lncRNAs in esophageal squamous cell carcinoma. [PDF]

, 2017
Long non-coding RNAs (lncRNAs) have a critical role in cancer initiation and progression, and thus may mediate oncogenic or tumor suppressing effects, as well as be a new class of cancer therapeutic targets. We performed high-throughput sequencing of RNA
Ai, B, Bai, X-F, Cao, H-H, Feng, C-C, He, J-Z, Huang, G-W, Koeffler, HP, Lei, F, Li, C-Q, Li, E-M, Li, M, Li, S, Li, X, Li, X-C, Liao, L-D, Lin, D-C, Liu, Y-J, Shen, J-H, Tang, Z-D, Wang, L-D, Wang, M-R, Wang, Q-Y, Wang, S-H, Wu, B-L, Wu, J-Y, Wu, Z-Y, Xu, L-Y, Xu, Y-J, Xue, Y-J, Zhang, J, Zhao, J-M, Zheng, C-P, Zhu, Y   +32 more
core   +1 more source

High-Quality and High-Yield RNA Extraction Method From Whole Human Saliva

Biomarker Insights, 2020
Background: Human saliva has been identified as a novel, practical, and noninvasive source of biomarkers and genetic materials. However, it is equally challenging due to the availability of an abundance of impurities in the form of microbes and other ...
Vaibhav Gandhi, Mara H. O’Brien, Sumit Yadav   +2 more
doaj   +1 more source

Stability investigation of air-dried olive ribo nucleic acids for metavirome studies

Plant Methods, 2022
Background The application of ribo nucleic acids for molecular studies requires high integrity and quality of extracted total RNA samples. In addition, the need to transfer RNA samples at room temperature without special treatments such as ice and liquid
Leila Mirzaei, Abbas Yadollahi, Maryam Jafarkhani Kermani, Masoud Naderpour, Ali Asghar Zeinanloo   +4 more
doaj   +1 more source

Macrophage migration inhibitory factor (MIF) family in arthropods : Cloning and expression analysis of two MIF and one D-dopachrome tautomerase (DDT) homologues in Mud crabs, Scylla paramamosain [PDF]

, 2016
Acknowledgements This research was supported by grants from the National Natural Science Foundation of China (Nos. 31172438 and U1205123), the Natural Science Foundation of Fujian Province (No.
Duan, Li-Peng, Huang, Bei, Huang, Wen-Shu, Jia, Qin-qin, Nie, Pin, Wang, Ke-Jian, Wang, Tiehui, Zhang, Cai-Liang   +7 more
core   +1 more source

Reverse chemical ecology approach for the identification of an oviposition attractant for Culex quinquefasciatus. [PDF]

, 2018
Pheromones and other semiochemicals play a crucial role in today's integrated pest and vector management strategies. These semiochemicals are typically discovered by bioassay-guided approaches.
Bhagavathy, Ganga, Chauhan, Kamlesh R, Choo, Young-Moo, Hwang, Justin K, Leal, Walter S, Tan, Kaiming, Xu, Pingxi, Zeng, Fangfang   +7 more
core   +1 more source

Total rRNA-Seq Analysis Gives Insight into Bacterial, Fungal, Protozoal and Archaeal Communities in the Rumen Using an Optimized RNA Isolation Method

Frontiers in Microbiology, 2017
Advances in high throughput, next generation sequencing technologies have allowed an in-depth examination of biological environments and phenomena, and are particularly useful for culture-independent microbial community studies.
Chijioke O. Elekwachi, Zuo Wang, Zuo Wang, Xiaofeng Wu, Alaa Rabee, Robert J. Forster   +5 more
doaj   +1 more source