Results 41 to 50 of about 461,258 (325)

Extraction RNA Protocol from Blood and Testis Tissue of Local Roosters

open access: yesTikrit Journal for Agricultural Sciences, 2021
The (one-step) method has become widely used to isolate total RNA from living organism samples and from different tissues. The aim of this study is to extract RNA from the blood and testicular tissue of male local chickens.
Ahmed Khalid   +3 more
doaj   +1 more source

Development of modified CTAB and Trizol protocols to isolate high molecular weight (HMW) RNA from polyphenol and polysaccharides rich pigeonpea (Cajanuscajan (L.) Millsp

open access: yesPLoS ONE, 2023
Pigeonpea (Cajanuscajan L.) is a legume crop that contains high levels of polyphenolic compounds and polysaccharides that become a hindrance in extracting good-quality and enough amount of RNA from its tissues. With the existing methods of RNA isolation,
Pawan Mainkar   +7 more
semanticscholar   +1 more source

An optimized method to obtain high-quality RNA from different tissues in Lilium davidii var. unicolor

open access: yesScientific Reports, 2022
The high quality, yield and purity total RNA samples are essential for molecular experiments. However, harvesting high quality RNA in Lilium davidii var.
Chunlei Wang   +7 more
doaj   +1 more source

Comparison of methods for pre-processing, exosome isolation, and RNA extraction in unpasteurized bovine and human milk.

open access: yesPLoS ONE, 2021
Milk is a highly complex, heterogeneous biological fluid that contains non-nutritive, bioactive extracellular vesicles called exosomes. Characterization of milk-derived exosomes (MDEs) is challenging due to the lack of standardized methods that are ...
Sanoji Wijenayake   +6 more
doaj   +1 more source

How Does the Sample Preparation of Phytophthora infestans Mycelium Affect the Quality of Isolated RNA?

open access: yesCurrent Issues in Molecular Biology, 2023
RNA isolation from fungi and fungus-like organisms is not an easy task. Active endogenous RNases quickly hydrolyze RNA after the sample collection, and the thick cell wall prevents inhibitors from penetrating the cells.
Artemii A. Ivanov   +2 more
doaj   +1 more source

CryoGrid-PIXUL-RNA: high throughput RNA isolation platform for tissue transcript analysis

open access: yesBMC Genomics, 2023
Background Disease molecular complexity requires high throughput workflows to map disease pathways through analysis of vast tissue repositories. Great progress has been made in tissue multiomics analytical technologies.
Scott A. Schactler   +10 more
doaj   +1 more source

Detection of MicroRNAs in Dried Serum Blots [PDF]

open access: yes, 2010
MicroRNAs are short RNAs which can be utilized as biomarkers for a variety of conditions. They are detectable in serum, and changes in the levels of circulating microRNAs have been associated with different diseases.
Reema Mallick   +2 more
core   +2 more sources

Increased Hematopoietic Extracellular RNAs and Vesicles in the Lung during Allergic Airway Responses. [PDF]

open access: yes, 2019
Extracellular RNAs (exRNAs) can be released by numerous cell types in vitro, are often protected within vesicles, and can modify recipient cell function. To determine how the composition and cellular sources of exRNAs and the extracellular vesicles (EVs)
Ansel, K Mark   +6 more
core   +1 more source

MicroRNA-483 amelioration of experimental pulmonary hypertension. [PDF]

open access: yes, 2020
Endothelial dysfunction is critically involved in the pathogenesis of pulmonary arterial hypertension (PAH) and that exogenously administered microRNA may be of therapeutic benefit.
Bai, Liang   +22 more
core   +1 more source

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