Action mechanisms and research methods of tRNA-derived small RNAs
Signal Transduction and Targeted Therapy, 2020 tRNA-derived small RNAs (tsRNAs), including tRNA-derived fragments (tRFs) and tRNA halves (tiRNAs), are small regulatory RNAs processed from mature tRNAs or precursor tRNAs.
Junming Guo
exaly +2 more sources
Cryo-EM of elongating ribosome with EF-Tu•GTP elucidates tRNA proofreading
Nature, 2020 Ribosomes accurately decode mRNA by proofreading each aminoacyl-tRNA that is delivered by the elongation factor EF-Tu 1 . To understand the molecular mechanism of this proofreading step it is necessary to visualize GTP-catalysed elongation, which has ...
Anna B Loveland +2 more
exaly +2 more sources
tRNA dysregulation and disease
Nature Reviews Genetics, 2022 Esteban A Orellana, Richard I Gregory
exaly +2 more sources
Discovery of Escherichia coli methionyl-tRNA synthetase mutants for efficient labeling of proteins with azidonorleucine in vivo [PDF]
, 2009 Incorporation of noncanonical amino acids into cellular proteins often requires engineering new aminoacyl-tRNA synthetase activity into the cell. A screening strategy that relies on cell-surface display of reactive amino acid side-chains was used to ...
Goddard, William A., III +4 more
core +6 more sources
Quantitative analysis of tRNA abundance and modifications by nanopore RNA sequencing
Nature Biotechnology, 2023 Transfer RNAs (tRNAs) play a central role in protein translation. Studying them has been difficult in part because a simple method to simultaneously quantify their abundance and chemical modifications is lacking.
M. Lucas +8 more
semanticscholar +1 more source
The tRNA identity landscape for aminoacylation and beyond
Nucleic Acids Research, 2023 tRNAs are key partners in ribosome-dependent protein synthesis. This process is highly dependent on the fidelity of tRNA aminoacylation by aminoacyl-tRNA synthetases and relies primarily on sets of identities within tRNA molecules composed of ...
R. Giegé, G. Eriani
semanticscholar +1 more source
Breaking the genetic code – a new revolutionary stage in the development of molecular biology: 1968 Nobel Prize laureates M. W. Nirenberg, H. G. Khorana, R. W. Holley [PDF]
The Ukrainian Biochemical Journal, 2021 This review presents the life stories of M. Nirenberg, H. Khorana, and R. Holley, winners of the 1968 Nobel Prize in Physiology or Medicine, the history of the discoveries made by these scientists, and the methodological approaches used in their works ...
O. P. Matyshevska +2 more
doaj +1 more source
Novel layers of RNA polymerase III control affecting tRNA gene transcription in eukaryotes [PDF]
Open Biology, 2017 RNA polymerase III (Pol III) transcribes a limited set of short genes in eukaryotes producing abundant small RNAs, mostly tRNA. The originally defined yeast Pol III transcriptome appears to be expanding owing to the application of new methods.
Ewa Leśniewska, Magdalena Boguta
doaj +1 more source
METTL1 promotes hepatocarcinogenesis via m7G tRNA modification‐dependent translation control
Clinical and Translational Medicine, 2021 Background N7‐methylguanosine (m7G) modification is one of the most common transfer RNA (tRNA) modifications in humans. The precise function and molecular mechanism of m7G tRNA modification in hepatocellular carcinoma (HCC) remain poorly understood ...
Zhi-hang Chen +14 more
semanticscholar +1 more source
Mechanisms of ribosome recycling in bacteria and mitochondria: a structural perspective
RNA Biology, 2022 In all living cells, the ribosome translates the genetic information carried by messenger RNAs (mRNAs) into proteins. The process of ribosome recycling, a key step during protein synthesis that ensures ribosomal subunits remain available for new rounds ...
Savannah M. Seely, Matthieu G. Gagnon
doaj +1 more source