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Elf5 regulation in the Trophectoderm
Mouse Elf5 is expressed exclusively in the trophectoderm from the late blastocyst stage to postgastrulation. We demonstrate here that the proximal promoter is used for trophectoderm expression but is not sufficient on its own. In transgenic assays, deletion of a differentially methylated region (DMR) within the promoter has no effect on the activation ...
David J Pearton +2 more
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Number of biopsied trophectoderm cells is likely to affect the implantation potential of blastocysts with poor trophectoderm quality [PDF]
To evaluate whether the developmental potential of the blastocyst is affected by the number of trophectoderm (TE) cells biopsied in preimplantation genetic diagnosis (PGD) cycles.Retrospective study.University-affiliated center.Women underwent PGD cycles of blastocyst biopsy and fluorescence in situ hybridization analysis.Not applicable.Biopsied TE ...
Shuoping Zhang, Keli Luo, Dehua Cheng
exaly +3 more sources
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Trophectoderm biopsy in human blastocysts
Human Reproduction, 1990Trophectoderm biopsy was carried out on 47 human blastocysts. A slit was made in the zona pellucida opposite the inner cell mass by micromanipulative techniques. The human blastocyst zona offered more resistance to slitting compared to that of the mouse. After 18-24 h, controlled herniation of the trophectoderm cells was observed.
A Dokras, I L Sargent, R L Gardner
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Elucidating the behavior of trophectoderm derivatives in mouse implantation
Developmental Cell, 2022Studying mammalian implantation in utero is difficult, but many in vitro models of peri-implantation development lack contributions from extra-embryonic tissues. Two recently published Developmental Cell papers present biomimetic systems for culturing peri-implantation mouse blastocysts ex vivo. These papers reveal dynamics and developmental impacts of
Aidan H, Terhune, Jianping, Fu
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Mosaicism in the mouse trophectoderm
Tissue and Cell, 1990The issue of mosaicism in the mouse trophectoderm is examined by reviewing two sets of evidence: one arguing for a mosaic, the other for a non-mosaic character. Evidence for mosaicism includes documented cellular contribution from the inner cell mass to the trophectoderm, and data that reveal the gradual pace of the allocation process that separates ...
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Trophectoderm cell subpopulations in the periimplantation mouse blastocyst
Journal of Experimental Zoology, 1984AbstractTrophectoderm of the preimplantation mouse blastocyst is composed of two cell subpopulations relative to their proximity to the inner cell mass. The polar trophectoderm overlying the inner cell mass proliferates to form the ectoplacental cone, and the mural trophectoderm endoreplicates and gives rise to giant cells.
D J, Chávez, A C, Enders, S, Schlafke
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Human trophectoderm biopsy and secretion of chorionic gonadotrohin
Human Reproduction, 1991We have previously developed a technique of trophectoderm biopsy to obtain cells from human blastocysts for preimplantation genetic diagnosis. To determine whether this technique affects the subsequent development of the blastocyst, 45 manipulated blastocysts were observed from days 3 to 14 in culture, the amount of human chorionic gonadotrophin (HCG ...
A, Dokras +3 more
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Radioiodinated surface proteins of rabbit trophectoderm cells
Cell And Tissue Research, 1986We have previously used surface iodination to discriminate between the protein patterns of epithelial cell surfaces in uteri of rabbits receptive (Day 6.5) or nonreceptive (Day 4) to nidation (Ricketts et al. 1984). In this paper, we describe application of the same technique to the trophoblastic surface of rabbit blastocysts collected on the same days
A P, Ricketts, D W, Scott, D W, Bullock
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Development, 1979
ABSTRACT Selective labelling of polar trophectoderm cells in early mouse blastocysts has allowed the fate of polar cells to be followed during in vitro and in vivo blastocyst development. Results show that there is cell movement from polar to mural regions as blastocysts grow. This indicates that trophectoderm cells directly opposite the
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ABSTRACT Selective labelling of polar trophectoderm cells in early mouse blastocysts has allowed the fate of polar cells to be followed during in vitro and in vivo blastocyst development. Results show that there is cell movement from polar to mural regions as blastocysts grow. This indicates that trophectoderm cells directly opposite the
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Mosaicism between trophectoderm and inner cell mass
Fertility and Sterility, 2017Defining the actual incidence and prevalence of mosaicism in human blastocysts still remains a difficult task. The small amount of evidence generated by animal and human studies does not support the existence of mechanisms involved in developmental arrest, clonal depletion, or aneuploidy rescue for abnormal cells in euploid/aneuploid embryos during ...
Antonio Capalbo, Laura Rienzi
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