Results 161 to 170 of about 4,779 (198)
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1977
The presence of an enzyme associated with tropoelastin is described. The enzyme has a pH optimum between 7 and 9 and trypsin-like specificity. Upon incubation, tropoelastin (72,000 molecular weight) is cleaved into relatively high molecular weight fragments. In addition to the parent molecule, five discrete polypeptide bands are usually observed on SDS
R, Mecham, J A, Foster, C, Franzblau
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The presence of an enzyme associated with tropoelastin is described. The enzyme has a pH optimum between 7 and 9 and trypsin-like specificity. Upon incubation, tropoelastin (72,000 molecular weight) is cleaved into relatively high molecular weight fragments. In addition to the parent molecule, five discrete polypeptide bands are usually observed on SDS
R, Mecham, J A, Foster, C, Franzblau
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Tropoelastin: A versatile, bioactive assembly module [PDF]
Acta Biomaterialia, 2014 Elastin provides structural integrity, biological cues and persistent elasticity to a range of important tissues, including the vasculature and lungs. Its critical importance to normal physiology makes it a desirable component of biomaterials that seek to repair or replace these tissues.
Steven G Wise +2 more
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Coacervation Characteristics of Recombinant Human Tropoelastin [PDF]
FEBS Journal, 1997 Coacervation of soluble tropoelastin molecules is characterized by thermodynamically reversible association as temperature is increased under appropriately juxtaposed ionic conditions, protein concentration and pH. Coacervation plays a critical role in the assembly of these elastin precursors in elastic fiber formation.
Sacha A Jensen, Anthony S Weiss
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Connective Tissue Research, 1991
Because tropoelastin is difficult to purify, most antibodies to elastin are raised against the insoluble form of the molecule. While these antibodies cross-react with tropoelastin, antigenic differences between insoluble and soluble elastin suggest that antibodies raised directly against tropoelastin might provide a more sensitive and specific reagent ...
I W, Prosser +6 more
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Because tropoelastin is difficult to purify, most antibodies to elastin are raised against the insoluble form of the molecule. While these antibodies cross-react with tropoelastin, antigenic differences between insoluble and soluble elastin suggest that antibodies raised directly against tropoelastin might provide a more sensitive and specific reagent ...
I W, Prosser +6 more
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Biosynthesis of Tropoelastin by Elastic Cartilage
Connective Tissue Research, 1980Elastic ear cartilage and aortae from nine-day-old hamsters were incubated in Krebs-Ringer medium containing ascorbate, beta-aminopropionitrile and either [14C]proline or [3H]valine. The [14C]proline-labeled proteins were separated by chromatography on Agarose A-5 m in sodium dodecyl sulfate and their [14C]-hydroxyproline content measured.
P, Heeger, J, Rosenbloom
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Characterization of rat heart tropoelastin
Archives of Biochemistry and Biophysics, 1989Several overlapping rat tropoelastin cDNA clones were isolated from a lambda gt11 rat heart cDNA library and their nucleotide sequence was determined. The corresponding deduced amino acid sequence of rat tropoelastin revealed strong homology to bovine and human tropoelastins although possessing some unique features including greater size (18%) and ...
C B, Rich, J A, Foster
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Fibrillation of tropoelastin induced by proteoglycan
Experientia, 1976Electrostatic interaction between tropoelastin, the native precursor of elastin, and proteoglycan results in tropoelastin fibrillation. The finding suggests a possible involvement of proteoglycans in elastogenesis.
M, Adam, V, Podrazký
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The International Journal of Biochemistry & Cell Biology, 2004
Fibulin-5 (also known as DANCE) is an elastin-binding protein that is thought to play a role in elastogenesis. We examined the relationship between the gene expression of fibulin-5 and the gene expression and accumulation of tropoelastin by comparing elastin-producing cells (human gingival fibroblasts) with non-elastin-producing cells (human ...
Eichi, Tsuruga +2 more
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Fibulin-5 (also known as DANCE) is an elastin-binding protein that is thought to play a role in elastogenesis. We examined the relationship between the gene expression of fibulin-5 and the gene expression and accumulation of tropoelastin by comparing elastin-producing cells (human gingival fibroblasts) with non-elastin-producing cells (human ...
Eichi, Tsuruga +2 more
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Primary Structure of Porcine Tropoelastin
1977Structural studies on porcine aortic tropoelastin as obtained from copper-deficient swine have been performed on the whole protein as well as fragments produced by trypsin, subtilisin and thrombin digestion. Tryptic fragments fall into two catagories, those large, over 20 residues, and those small, 4 residues and less.
L B, Sandberg +5 more
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Flexibility in the Solution Structure of Human Tropoelastin
Biochemistry, 2007We investigated the flexibility of full-length tropoelastin in solution by using far- and near-ultraviolet circular dichroism (UV CD) and fluorescence spectroscopy to probe for structural flexibility and residue mobility within secondary and tertiary features of the monomer.
Lisa D, Muiznieks, Anthony S, Weiss
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