Results 181 to 190 of about 16,282 (199)

The specificity of transcription in vitro of the trp operon of Escherichia coli

Molecular and General Genetics MGG, 1973
The transcription in vitro of the tryptophan (trp) operon of Escherichia coli was studied with a preparation consisting of purified RNA polymerase from E. coli and purified DNA from a trp transducing strain of ϕ80. The synthesis of trp mRNA was determined a) in a two-step RNA-DNA filter hybridization test (measurement of the amount of RNA which remains
Peter H. Pouwels, Hans Pannekoek
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Derepression of E. coli trp operon on interfamilial transfer

Nature, 1977
THE P-group R factor RP4 originally isolated from Pseudomonas aeruginosa can be transferred to many Gram-negative bacteria1–3. In spite of its large molecular weight, RP4 has only one site susceptible to each of EcoRI (ref. 4), HindIII (ref. 5) and BamNI (data not shown) restriction endonucleases.
Kenji Sakaguchi, Kenji Nagahari, Yumiko Sano   +2 more
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Replacement of the Escherichia coli trp operon attenuation control codons alters operon expression

Journal of Molecular Biology, 1990
To test features of the current model of transcription attenuation in amino acid biosynthetic operons, alterations were introduced into the trp operon leader region and expression of the mutated operons was examined in miaA and miaA+ Escherichia coli strains that lacked the trp repressor. The miaA mutation prevents modification of the adenosine residue
Charles Yanofsky, Karen Choo, Le Phung, Robert Landick   +3 more
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Evidence of read-through at the termination signal for transcription of the trp operon

Virology, 1976
Abstract We reported earlier that the polarity induced by nonsense mutations in the trp operon is relaxed when the trp mRNA is part of a long transcript from the phage λ promoter P L . Here we report that in addition, the transcription initiated at the P L promoter of phage λ continues into the region beyond the operator-distal end of ...
Takashi Segawa, Fumio Imamoto
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Quantitative aspects of gene expression in a λ-trp fusion operon

Molecular and General Genetics MGG, 1974
A λptrp phage is described in which the five genes for tryptophan biosynthesis are expressed from the leftward promoter of λ and are controlled by the products of λ genes N, cI and cro. From a lysogen of this phage in a strain carrying a deletion for the trp genes derivatives were made that are able to exist in the immune (im+), anti-immune (im−) and ...
Françoise Brunel, John Davison, John Davison, William J. Brammar   +3 more
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Genetic fusions defining trp and lac operon regulatory elements

Journal of Molecular Biology, 1975
Abstract Two procedures for easily isolating deletions that fuse the trp and lac operons are described. Using these procedures, a large number of fusion deletions have been isolated. The lac ends of these deletions extend varying distances into the lacI gene and the lac promoter-operator region.
David H. Mitchell, Jon Beckwith, William S. Reznikoff   +2 more
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Identification of trp-p2, an internal promoter in the tryptophan operon of Escherichia coli

Journal of Molecular Biology, 1982
Abstract Previous genetic studies localized an internal low efficiency promoter, called p2, within the distal portion of the D gene of the tryptophan (trp) operon of Escherichia coli. The nucleotide sequence of trpD reveals a promoter-like region about 150 nucleotides from the 3′ end of the gene. We report here the results of transcription studies in
Heidi Horowitz, Terry Platt
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Cycling expression and cooperative operator interaction in the trp operon of Escherichia coli

Journal of Theoretical Biology, 2010
Oscillatory behaviour in the tryptophan operon of an Escherichia coli mutant strain lacking the enzyme-inhibition regulatory mechanism has been observed by Bliss et al. but not confirmed by others. This behaviour could be important from the standpoint of synthetic biology, whose goals include the engineering of intracellular genetic oscillators.
Eduardo S. Zeron, Areli Hernández-Valdez, Moisés Santillán   +2 more
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Regulatory features of the trp operon and the crystal structure of the trp RNA-binding attenuation protein from Bacillus stearothermophilus

Journal of Molecular Biology, 1999
Characterization of both the cis and trans -acting regulatory elements indicates that the Bacillus stearothermophilustrp operon is regulated by an attenuation mechanism similar to that which controls the trp operon in Bacillus subtilis. Secondary structure predictions indicate that the leader region of the trp mRNA is capable of folding into terminator
Pan T.X. Li, Min Yang, Paul Gollnick, Guy Dodson, Chris Baumann, Xiao-ping Chen, Eleanor J. Dodson, Alfred A. Antson   +7 more
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A mutation to 5-methyltryptophan dependence in the trp operon of Salmonella typhimurium

Molecular and General Genetics MGG, 1978
The AS-PRT enzyme complex which catalyzes the first two steps in the biosynthesis of tryptophan in S. typhimurium consists of two polypetide subunits: anthranilate synthetase (component I or AS-CoI) and anthranilate 5-phosphoribosylpyrophosphate phosphoribosyltransferase (PRT).
Elias Balbinder, Robert Torget, Frank Angelosanto   +2 more
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