Electron microscopic visualization of trp operon expression in Salmonella typhimurium. [PDF]
Transcriptional activity of plasmids carrying wild-type and mutant trp operons was visualized in cell lysates of Salmonella typhimurium. Plasmid and transcription-unit sizes varied with the size of the cloned operon. Following 3-(3-indolyl)acrylic acid derepression, all operons of a particular type exhibited the same high level of transcriptional ...
Ronald Bauerle+4 more
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Regulatory elements common to the Bacillus pumilus and Bacillus subtilis trp operons [PDF]
The trp operon regulatory region of Bacillus pumilus was cloned and sequenced. The cloned B. pumilus trp promoter-leader region functioned in Bacillus subtilis to express the adjacent leukocyte interferon A gene on a multicopy transcriptional fusion plasmid, pBpIFI.
Mitzi I. Kuroda+3 more
openalex +4 more sources
The mtr locus is a two-gene operon required for transcription attenuation in the trp operon of Bacillus subtilis. [PDF]
We have cloned and characterized the mtr operon of Bacillus subtilis. This operon encodes a presumed RNA-binding regulatory protein that is required for attenuation control of the trp operon. We have shown that the mtr operon consists of two structural genes, mtrA and mtrB, predicted to encode 22-kDa and 8-kDa polypeptides, respectively.
D J Henner+4 more
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A Unique Tryptophan C‐Prenyltransferase from the Kawaguchipeptin Biosynthetic Pathway [PDF]
Acknowledgements This work was supported by funding of the Academy of Finland (259505), Helsinki University Research grant (490085) and ESCMID grant (4720572) to D.P.F., University of Pittsburgh Central Research Development Fund to X.L., Technology ...
Arnison+21 more
core +2 more sources
Morphological development and cytochrome c oxidase activity in Streptomyces lividans are dependent on the action of a copper bound Sco protein [PDF]
Copper has an important role in the life cycle of many streptomycetes, stimulating the developmental switch between vegetative mycelium and aerial hyphae concomitant with the production of antibiotics.
Calabrese L+5 more
core +2 more sources
Rapidly changing concentrations of substrates frequently occur during large-scale microbial cultivations. These changing conditions, caused by large mixing times, result in a heterogeneous population distribution.
Alexander Nieß+3 more
doaj +1 more source
Evolution of substrate specificity in a recipient's enzyme following horizontal gene transfer [PDF]
Despite the prominent role of horizontal gene transfer (HGT) in shaping bacterial metabolism, little is known about the impact of HGT on the evolution of enzyme function.
Abascal+54 more
core +1 more source
From DNA sequence to application: possibilities and complications [PDF]
The development of sophisticated genetic tools during the past 15 years have facilitated a tremendous increase of fundamental and application-oriented knowledge of lactic acid bacteria (LAB) and their bacteriophages.
A Bruttin+110 more
core +3 more sources
The Bacillus cereus GerN and GerT protein homologs have distinct roles in spore germination and outgrowth, respectively [PDF]
The GerT protein of Bacillus cereus shares 74% amino acid identity with its homolog GerN. The latter is a Na+/H+-K+ 19 antiporter that is required for normal spore germination in inosine.
Moir, A., Senior, A.
core +2 more sources
Phenotypic Stability of trp Operon Recombinant Plasmids in Escherichia coli
The recombinant plasmids RSF2124-trp and pSC101-trp were examined for their phenotypic stability in Escherichia coli W3110 and its derivatives under various culture conditions. RSF2124-trp and pSC101-trp were stable in a trpAE1 strain. In an amber mutant of the tryptophan repressor gene, RSF2124-trp was fairly stable, whereas pSC101-trp was unstable ...
Hiroshi Tsunekawa+2 more
openaire +3 more sources