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Development and pilot application of a point-of-need molecular xenomonitoring protocol for tsetse (Glossina sp.) in a low-resource setting. [PDF]

PLoS Negl Trop Dis
Saldanha I, Aziku E, Trima AT, Drapari V, Garrod G, Ombanya H, Tirados I, Betson M, Mugenyi A, Dunkley S, Torr SJ, Hope A, Cunningham LJ.   +12 more
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Trypanosoma congolense: Isolation and purification

Experimental Parasitology, 1979
Abstract Yields of Trypanosoma congolense grown in rats may be increased by placing the rats in a 37 °C environment for 1 hr prior to sacrifice. A further increase in the number of parasites recovered per rat may be achieved by replacement of blood removed by a lactated Ringer's solution with 5% glucose as the rat is being bled from the abdominal ...
N L, Rosen, M, Onodera, C L, Patton, M B, Lipman, F F, Richards   +4 more
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Multiplication of Trypanosoma brucei and Trypanosoma congolense in vertebrate hosts

Transactions of the Royal Society of Tropical Medicine and Hygiene, 1969
Abstract Studies on multiplication of T. brucei and T. congolense showed that trypanosomes artificially maintained in mice multiply exclusively by longitudinal fission. T. brucei and T. congolense in a rabbit and a guinea-pig as well as in sheep has, in addition to multiplication by binary fission, a developmental cycle which includes ...
M A, Soltys, P, Woo
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An extravascular site of development of Trypanosoma congolense

Nature, 1978
TSETSE-TRANSMITTED African pathogenic trypanosomes are thought to be divisible into two groups because of their distribution in the mammalian host and the characteristic lesions they produce during infection1. Trypanosoma brucei brucei and related subspecies have a wide distribution in the body, parasitising the intercellular fluids, connective tissue ...
A G, Luckins, A R, Gray
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Trypanosoma congolense infection in two dogs

Journal of Small Animal Practice, 1995
ABSTRACTTrypanosomiasis, caused by Trypanosoma congolense, was diagnosed for the first time in Israel in two boxer dogs imported from Kenya. The dogs developed clinical signs two days after arrival and succumbed to the disease within four days. The major clinical and clinicopathological findings included anaemia, haemorrhages, lymphadenomegaly ...
S, Harrus, A, Harmelin, B, Presenty, H, Bark   +3 more
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Effect of isometamidium on Trypanosoma congolense infectivity

Veterinary Parasitology, 1991
Isometamidium chloride (Samorin, RMB, England) is a widely used and highly effective trypanocide for the treatment of bovine trypanosomiases. However, the appearance of isometamidium-resistant populations of T. congolense in Africa makes it necessary to develop methods for the rapid and reliable detection of drug resistance in the laboratory. Currently
I A, Sutherland, A, Mounsey, P H, Holmes
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Trypanosoma congolense: Calf erythrocyte survival

Experimental Parasitology, 1979
Abstract Hereford calves infected with Trypanosoma congolense developed an anemia which was most severe 10 weeks after infection when packed cell volumes (PCV) averaged 21.1 ± 2.5% (±2 SE) as compared to 33.1 ± 2.1% for controls. At the termination of the study, at 28 weeks postinfection PCVs of infected animals had risen to 27.5 ± 1.0% as compared ...
J M, Preston, B T, Wellde, R M, Kovatch
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Studies on the carbohydrate metabolism of Trypanosoma congolense

Experimental Parasitology, 1954
Abstract 1. 1. The rate of oxygen consumption of T. congolense is nearly as high as that of the trypanosomes of the evansi-brucei groups, but its rate of glucose consumption is lower. 2. 2. The R.Q. is high. 3. 3. The main metabolic end products, accounting nearly quantitatively for the degraded glucose, are acetic acid, pyruvic acid,
M, AGOSIN, T, VON BRAND
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Expression of foreign proteins in Trypanosoma congolense

Molecular and Biochemical Parasitology, 1999
An expression vector was constructed to express foreign genes in Trypanosoma congolense. The foreign gene and a neomycin phosphotransferase (NPT) gene are flanked by glutamate and alanine rich protein (GARP) gene processing signals and their expression is driven by a ribosomal RNA gene promoter.
N, Downey, J E, Donelson
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The generation and identification of the hemolysin of Trypanosoma congolense

Experientia, 1977
The hemolytic activity of Trypanosoma congolense appears to be due to the presence of free fatty acids generated by the action of phospholipase A on endogenous phosphatidyl choline. Some lysolecithin also contributes to the lytic activity. Trypanosoma lewisi, being devoid of phospholipase A, does not generate free fatty acids and is therefore non ...
I R, Tizard, W L, Holmes, D A, York, A, Mellors   +3 more
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