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Genetic Techniques in Trypanosoma cruzi
2011It is almost 20 years since genetic manipulation of Trypanosoma cruzi was first reported. In this time, there have been steady improvements in the available vector systems, and the applications of the technology have been extended into new areas.
Martin C, Taylor +2 more
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The γ-glutamyltranspeptidase of Trypanosoma cruzi
Comparative Biochemistry and Physiology Part B: Comparative Biochemistry, 1987Trypanosoma cruzi epimastigotes show gamma-glutamyltranspeptidase activity which has characteristics significantly different than the mammalian enzyme. The protozoan enzyme is localized in the cytosolic fraction, it has a Km of 1.6 mM and a Vmax of 17.4 nmol/min/mg protein with L-gamma-glutamyl-p-nitroanilide as gamma-glutamyl donor, and an optimun pH ...
Y, Repetto +3 more
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Characterization of Trypanosoma cruzi hexokinase
Molecular and Biochemical Parasitology, 1983Properties of hexokinase (EC 2.7.1.1) from Trypanosoma cruzi epimastigote forms (Tulahuen strain) were studied and compared with enzymes from other sources. The enzyme activity was 37 units g-1 of wet cells (1.2 units mg-1 protein). Hexokinase showed Km values for glucose and ATP of 0.09 and 0.4 mM, respectively.
G E, Racagni, E E, Machado de Domenech
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An immunocytotoxic assay for Trypanosoma cruzi
International Journal for Parasitology, 1976Abstract Trypanosoma cruzi culture forms grown for 8 days in LIT medium fortified with 14C-labeled amino acids will incorporate the isotope into membranous and soluble components of the parasite. On lysis, labeled cytoplasmic elements released into the surrounding medium can be measured and reflect the degree of destruction of the parasites ...
R E, Kuhn, R T, Vaughn
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On the DNA content of Trypanosoma cruzi
Molecular and Biochemical Parasitology, 1989The DNA contents of three different Trypanosoma cruzi strains were compared by direct microfluorometry. The maximal difference found was 40% of the lowest value. Two of the cloned strains, reported in an earlier study to differ by 48% in their total DNA content, showed a difference of 33%.
R F, Kooy +3 more
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Karyotype variability in Trypanosoma cruzi
Parasitology Today, 1996Like many other protozoam parasites, Trypanosoma cruzi (the causative agent of Chagas disease) has a plastic genome. Chromosome size polymorphisms occur in different strains of T. cruzi as well as among clones originating from the same strain, Despite this polymorphism, major interchromosomal rearrangements appear to be rare since several linkage ...
J, Henriksson, L, Aslund, U, Pettersson
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Intermediary metabolism of Trypanosoma cruzi
Parasitology Today, 1994In this article, Julio Urbino discusses the characteristics o f the intermediary metabolism of Trypanosoma cruzi (the causative agent of Chagas disease), which are responsible for the unusual capacity of this parasite to use carbohydrates or amino acids as carbon and energy sources without drastic changes in its catabolic enzyme levels(1-3).
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Zymodeme characterization of Trypanosoma cruzi
Parasitology Today, 1986La repartition geographique des symptomes de la maladie n'est pas uniforme et differents isolats de parasites presentent de grandes variations dans leurs caracteristiques biologiques. Cet article analyse le profil isoenzymatique et l'ADN cinetoplastique afin de determiner l'heterogeneite de ces intriguants ...
M A, Miles, R E, Cibulskis
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Cell Biology of Trypanosoma cruzi
1984Publisher Summary Among the protozoa of the Trypanosomatidae family, a large number of species represent agents of diseases, such as Chagas' disease. This chapter reviews some aspects of the cell biology of Trypanosoma cruzi, giving emphasis to those aspects related to the ultrastructure of pathogenic protozoa. Protozoa of the Trypanosomatidae family
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Nuclear Structure of Trypanosoma cruzi
2011The presence of nucleus in living organisms characterizes the Eukaryote domain. The nucleus compartmentalizes the genetic material surrounded by a double membrane called nuclear envelope. The nucleus has been observed since the advent of the light microscope, and sub-compartments such as nucleoli, diverse nuclear bodies and condensed chromosomes have ...
Sergio, Schenkman +2 more
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