Results 361 to 370 of about 736,972 (389)

Large-scale quantitative assessment of different in-solution protein digestion protocols reveals superior cleavage efficiency of tandem Lys-C/trypsin proteolysis over trypsin digestion.

Journal of Proteome Research, 2012
The complete and specific proteolytic cleavage of protein samples into peptides is crucial for the success of every shotgun LC-MS/MS experiment. In particular, popular peptide-based label-free and targeted mass spectrometry approaches rely on efficient ...
Timo Glatter, C. Ludwig, Erik Ahrné, R. Aebersold, A. Heck, A. Schmidt   +5 more
semanticscholar   +1 more source

A radioactive method for the measurement of trypsin and trypsin-like activities

Analytical Biochemistry, 1989
A simple and highly sensitive method for the assay of trypsin has been developed by making use of the phosphorylated synthetic peptide Leu-Arg-Arg-Ala-Ser-(32P)-Leu-Gly as substrate. The technique has been adapted from the phosphocellulose method of R. Roskoski, Jr. (in Methods in Enzymology (Corbin, J., and Hardman, J., Eds.), Vol. 99, pp.
Susana Passeron, P. Fernandez Murray, Maria Leonor Cantore, Susana Silberstein   +3 more
openaire   +3 more sources

Interaction between four flavonoids and trypsin: effect on the characteristics of trypsin and antioxidant activity of flavonoids

, 2014
Summary Interaction of flavonoids and enzyme may affect characteristics and physiological activities of both components. In this study, the effects of the interaction between four flavonoids (quercetin, luteolin, kaempferol and apigenin) and trypsin ...
Qiong Li, Qingyi Wei, Erdong Yuan, Jiguo Yang, Zheng-xiang Ning   +4 more
semanticscholar   +1 more source

Novel trypsin-FITC@MOF bioreactor efficiently catalyzes protein digestion.

Journal of materials chemistry. B, 2013
A simple nanoporous metal organic framework (MOF) bioreactor preparation via a low energy 30 minute vortex as driving force to immobilize trypsin enzyme is presented. No chemical modifications were employed thus organic wastes were eliminated.
Wan-Ling Liu, S. Lo, Brenda Singco, Chun-Chuen Yang, Hsi-Ya Huang, Chia‐Her Lin   +5 more
semanticscholar   +1 more source

TRYPSIN AND TRYPSIN INHIBITORS FROM PENAEID SHRIMP

Journal of Food Biochemistry, 2002
Trypsin inhibitors from four species of shrimp, P. vannamei, P. monodon, P. stylirostris, and P. californiensis were characterized by test tube and electrophoretic assays. Inhibitors in the four phylogenetically related species showed different inhibitory capacity and specificity for orthologous trypsins. Inhibitors from P.
Cristiane De Albuquerque-Cavalcanti, M. Angeles Navarrete del Toro, Fernando García-Carreño   +2 more
openaire   +2 more sources

Self-assembled graphene quantum dots induced by cytochrome c: a novel biosensor for trypsin with remarkable fluorescence enhancement.

Nanoscale, 2013
On the basis of cytochrome c-induced self-assembled graphene quantum dots, we demonstrate a novel fluorescent biosensor for trypsin with remarkable fluorescence enhancement, as well as high selectivity and sensitivity.
Xing Li, Shoujun Zhu, Bin Xu, Ke Ma, Junhu Zhang, Bai Yang, Wenjing Tian   +6 more
semanticscholar   +1 more source

Tumour-associated trypsin inhibitor and tumour-associated trypsin

Scandinavian Journal of Clinical and Laboratory Investigation, 1990
Tumour-associated trypsin inhibitor (TATI) is a 6 kDa peptide, which is synthesized at low concentrations by several tumours and cell lines. Very high concentrations of TATI occur in mucinous ovarian tumours. Elevated levels of TATI occur in serum and urine in connection with most types of cancer at advanced stages. In mucinous ovarian cancer up to 85%
openaire   +3 more sources

Phase transfer surfactant-aided trypsin digestion for membrane proteome analysis.

Journal of Proteome Research, 2008
We have developed a new protocol for digesting hydrophobic proteins using trypsin with the aid of phase-transfer surfactants (PTS), such as sodium deoxycholate (SDC). SDC increases the solubility of hydrophobic proteins, enhances the activity of trypsin,
Takeshi Masuda, M. Tomita, Y. Ishihama
semanticscholar   +1 more source

The Origin of Trypsin: Evidence for Multiple Gene Duplications in Trypsins

Journal of Molecular Evolution, 1998
The trypsin family of serine proteases is one of the most studied protein families, with a wealth of amino acid sequence information available in public databases. Since trypsin-like enzymes are widely distributed in living organisms in nature, likely evolutionary scenarios have been proposed.
Baptista, A. M., Jonson, P. H., Hough, E., Petersen, S. B.   +3 more
openaire   +4 more sources

On the Potential Role of Trypsin and Trypsin Inhibitors in Acute Pancreatitis

1984
The protective role of alpha 2-macroglobulin, alpha 1-antitrypsin and Aprotinin against trypsin-induced effects on C3 and kininogen was studied in a human in vitro model. When human cationic trypsin was added to human serum or plasma, there was a gradual saturation of alpha 2-macroglobulin and later of alpha 1-antitrypsin.
Åke Lasson, Kjell Ohlsson
openaire   +3 more sources