Results 61 to 70 of about 155,237 (261)

Importin 7 mediates the nuclear import of HIV‐1 integrase via a specific interacting interface

FEBS Open Bio, EarlyView.
HIV‐1 integrase enables viral DNA integration into the host genome. By binding to the core domain of the host protein Importin 7 via its C‐terminal domain, the integrase is transported across the nuclear membrane into the nucleus, where integration of the viral genome into host DNA takes place. This translocation is a critical step for subsequent viral
Juana Bana, Avigail Yariv, Tal Oppenheim, Hadar Amartely, Dana Reichmann, Oded Livnah   +5 more
wiley   +1 more source

The Reactive Site of Trypsin Inhibitors

Journal of Biological Chemistry, 1966
Virgin soybean trypsin inhibitor has 1 NH2-terminal aspartic acid or asparagine residue and no COOH-terminal residues that can be released by carboxypeptidase B. Upon incubation with 1 mole % of trypsin for 24 hours at pH 3.75, virgin inhibitor is converted to a modified inhibitor which has 2 NH2 terminals aspartic acid or asparagine and isoleucine ...
K, Ozawa, M, Laskowski
openaire   +2 more sources

Dose‐dependent hepatotoxicity of hydrogen peroxide in HepG2 cells and its modulation by CYP450 induction

FEBS Open Bio, EarlyView.
NMR metabolomics revealed concentration‐dependent metabolic perturbations in HepG2 cells exposed to H2O2. Rifampicin pretreatment enhanced metabolic competence, attenuated toxin‐induced alterations and produced metabolite profiles more consistent with human liver physiology, supporting the use of CYP450‐induced HepG2 models for improved in vitro ...
Maren Jinks, Garth L. Maker, Emily C. Davies, Berin A. Boughton, Samantha Lodge   +4 more
wiley   +1 more source

EFFECTIVENESS INACTIVATION OF TRYPSIN INHIBITOR FROM BRAZILIAN CULTIVARS OF BEANS (PHASEOLUS VULGARIS L.)

Alimentos e Nutrição, 2011
The trypsin inhibitor, an antinutritional factor, which is abundant in dycotiledoneous and monocotyledoneous, is usually inactivated by heating treatment.
Kelli Cristina PAIVA, Maria Regina Barbieri de CARVALHO, João Martins PIZAURO JUNIOR   +2 more
doaj  

Identification of (20R)‐protopanaxadiol from Panax ginseng as a novel anti‐SARS‐CoV‐2 compound

FEBS Open Bio, EarlyView.
We established a noninfectious BAC‐based SARS‐CoV‐2 replicon that enables antiviral screening under BSL‐2 conditions. Using this platform, we screened 373 food‐derived compounds and identified (20R)‐protopanaxadiol from Panax ginseng as a novel inhibitor of SARS‐CoV‐2 RNA replication, highlighting the value of safe replicon systems for antiviral ...
Midori Takeda, Natsue Maezono, Rina Uchikoshi, Nobuyuki Kato, Masanori Ikeda   +4 more
wiley   +1 more source

Oligosaccharides, phytate and trypsin inhibitors in sweet lupine seeds (Lupinus angustifolius L.) and commercial products derived from them

Discover Food
Lupine seeds (Lupinus angustifolius) are increasingly recognized as a valuable source of plant-based protein for human nutrition. Similar to other legumes, lupines contain antinutrients.
Benno F. Zimmermann, Timo Heinrichs, Hedwig Heuser, Ismail-Hakkı Acır, Ute Schweiggert-Weisz   +4 more
doaj   +1 more source

Protocol for quantifying miRNA trafficking across the endosomal membrane

FEBS Open Bio, EarlyView.
An in vitro protocol measures miRNA uptake into endosomes isolated from mammalian cell extracts, which are free of subcellular contaminants. Performed at 37 °C in the presence of ATP, it ensures the import of single‐stranded miRNA into the endosomal lumen.
Syamantak Ghosh, Kamalika Mukherjee, Suvendra N. Bhattacharyya   +2 more
wiley   +1 more source

A Simple, Quantitative Method for Titration of Trypsin and Trypsin Inhibitors.

Experimental Biology and Medicine, 1949
SummaryA technically simple, rapid method for the quantitative titration of trypsin or similar proteolytic enzymes, and of protease inhibitors, suitable for critical tests on a large scale is described. The procedure utilizes technics familiar to serologists, rather than to skilled biochemists only, and gives definitive, reproducible results that can ...
K L, BURDON, R P, MUDD
openaire   +2 more sources

ALDOA Promotes Glycolysis and NLRP3/GSDMD Pyroptosis to Accelerate ALS Progression

Annals of Clinical and Translational Neurology, EarlyView.
ABSTRACT Objective Amyotrophic lateral sclerosis (ALS) is characterized by progressive motor neuron degeneration. Glycolytic dysregulation is implicated in disease progression, yet the underlying mechanisms remain unclear. This study investigates how Aldolase A (ALDOA) drives ALS progression through glycolysis‐mediated motor neuron pyroptosis.
Kaixin Yan, Yan Jiang, Yuxuan Yong, Tianshuo Zhang, Niannian Zhang, Qianqian Zeng, Xue Gong, Li Meng, Fangfang Bi, Yongmin Liu   +9 more
wiley   +1 more source

Differential Inhibition of Human Neutrophil Elastase and Bacterial Elastase by endogenous Protease Inhibitors

Journal of Clinical and Biomedical Sciences, 2018
Human Neutrophil Elastase (HNE) is released from neutrophils when foreign substances are encoun-tered. Elastase is also released by invasive pathogenic microorganisms and is an important virulence factor.
Deena Mendez, Prasad SR, Kutty AVM
doaj   +1 more source