Results 61 to 70 of about 497,491 (219)
Optimizing photoactivation of PA‐mCherry for optical pooled CRISPR screens
FEBS Open Bio, EarlyView.Photoactivatable PA‐mCherry finds widespread use to optically tag individual cells. However, confocal 405 nm UV laser‐scanning (normal scan) is much less efficient than widefield UV illumination, limiting the use of PA‐mCherry on confocal instruments. We remedy this limitation by reporting that rapid and repeated confocal scanning with a low‐intensity,
Sravasti Mukherjee +3 more
wiley +1 more source
Metalurgija, 2014 The companies from the metallurgical industry had had and have the capacity of contributing to the economic development of a country, and one of the most important aspects that have a crucial importance over the evolution and development of these ...
C. Căruntu, L. M. Lăpăduşi
doaj
Turnover and heterogeneity in top management networks - A demographic analysis of two Swedish business groups [PDF]
International Journal of Business Science and Applied Management, 2008 A theory based on the demography of top management teams is used to explain membership turnover in two Swedish business groups, network analysis being used to define group membership.
Collin, S. & Umans, T.
doaj
Diversity, 2017 The theory of island biogeography has revolutionised the study of island biology stimulating considerable debate and leading to the development of new advances in related areas.
Duncan McCollin
doaj +1 more source
RoundMi: A quantitative method to analyze mitochondrial morphology in mitotic cells
FEBS Open Bio, EarlyView.RoundMi is a workflow for rapid analysis of mitochondrial morphology in mitotic cells. By combining adaptive preprocessing with automated segmentation and quantification, it enables accurate measurements from single focal plane images, reducing acquisition time and computational demands while remaining compatible with high‐throughput fixed and live ...
Elmira Parvindokht Bararpour +2 more
wiley +1 more source
Directed evolution of enzymes at the crossroads of tradition and innovation
FEBS Open Bio, EarlyView.An iterative cycle of data‐driven enzyme optimization comprising four stages: genetic diversification of a template enzyme, expression of protein variants, high‐throughput evaluation, and machine‐learning‐guided redesign of the next variant library.
Maria Tomkova +2 more
wiley +1 more source
The Impact of Land-Use Change on the Diversity of Soil Macroinvertebrates in Rangelands and Hand-Planted Forests (Lavizan Forest Park, Tehran, Iran) [PDF]
محیط زیست و توسعه فرابخشیIntroduction: Rational use and protection of natural grasslands within legal frameworks to regulate rangeland management and prevent land degradation, especially in countries with extensive grasslands, are crucial. Land-use change from grasslands to hand-
Maryam Azimi bidar +4 more
doaj +1 more source
Small RNA pathways in mammalian oocytes
FEBS Open Bio, EarlyView.Three distinct small RNA pathways operate in mammalian oocytes: RNAi interference (RNAi), the microRNA (miRNA) pathway, and the PIWI‐associated RNA (piRNA) pathway. These pathways use small RNAs to guide sequence‐specific repression and contribute to oocyte biology by targeting genes and mobile elements or appear insignificant since different ...
Petr Svoboda, Josef Pasulka
wiley +1 more source
Transcripts enriched in codons that trigger P‐site tRNA‐mediated mRNA decay possess stable mRNA
FEBS Open Bio, EarlyView.PTMD codons were first described by Mendel et al. as mediators of an mRNA decay pathway dependent on the human protein CNOT3, homologous to yeast Not5. Our findings confirm that PTMD codons destabilize transcripts; however, unlike in yeast, the human pathway specifically targets and slightly destabilizes primarily stable mRNAs.
Rodolfo Lopes Carneiro +1 more
wiley +1 more source
Frontiers in Endocrinology, 2019 Objective: The purpose of our study was to evaluate the alterations of bone metabolism and the prevalence of vertebral fractures in the population with HIV and hepatitis B and C seropositivity in treatment with antiretroviral drugs (HAART).Methods: We ...
Elisa Dalla Grana +7 more
doaj +1 more source