Results 151 to 160 of about 212,059 (288)

Topological patterns in two-dimensional gel electrophoresis of DNA knots. [PDF]

Proc Natl Acad Sci U S A, 2015
Michieletto D, Marenduzzo D, Orlandini E.   +2 more
europepmc   +1 more source

Protein Structuromics Reveals a Loop‐Controlled Half‐Open Active Pocket Conformation Throughout Fe(II)/α‐ketoglutarate‐Dependent Dioxygenase Catalytic Cycle

Advanced Science, EarlyView.
This study reveals a conserved half‐open active pocket in Fe(II)/α‐ketoglutarate‐dependent dioxygenases through protein structuromics, distinguished by structural features comprising highly flexible loop elements. Enhanced sampling simulations‐guided mutagenesis experiments elucidated how this flexible loop regulates pocket open/closed conformations ...
Lunjie Wu, Huan Liu, Songyin Zhao, Lei Qin, Jiarui Li, Jun Wang, Zixuan Dai, Jie Gu, Yan Xu, Feiran Li, Yao Nie   +10 more
wiley   +1 more source

Trichinella britovi muscle larvae and adult worms: stage-specific and common antigens detected by two-dimensional gel electrophoresis-based immunoblotting. [PDF]

Parasit Vectors, 2018
Grzelak S, Moskwa B, Bień J.
europepmc   +1 more source

Analysis of Sertoli cell-secreted proteins by two-dimensional gel electrophoresis.

Biology of Reproduction, 1982
C. Kissinger, Michael K. Skinner, Michael D. Griswold   +2 more
semanticscholar   +1 more source

SuFEx‐Enabled Reprogramming of Flavonoids for Selective α‐Glucosidase Covalent Inhibition

Advanced Science, EarlyView.
Selective inhibition of intestinal α‐glucosidase remains limited by poor enzyme specificity and off‐target metabolic effects. Here, SuFEx click chemistry is used to reprogram natural flavonoids into covalent inhibitors with enhanced α‐glucosidase selectivity and controlled reactivity. This strategy enables effective regulation of carbohydrate digestion
Fengyu Guo, Liwei Zhang, Minlong Wang, Yanjin Du, Tianyu Zhang, Hao Chen, Jiazeng Sun, Yang Yu, Zude He, Jie An, Xiaoxu Zhang, Weilin Lin, Fazheng Ren, Pengjie Wang, Ping Liu   +14 more
wiley   +1 more source

Analysis of protein expression in Brucella abortus mutants with different growth rates by two-dimensional gel electrophoresis and LC-MS/MS peptide analysis. [PDF]

J Vet Sci, 2018
Park WB, Im YB, Shim S, Yoo HS.
europepmc   +1 more source

Invariance and heterogeneity in the major structural and regulatory proteins of chick muscle cells revealed by two-dimensional gel electrophoresis.

Proceedings of the National Academy of Sciences of the United States of America, 1977
J. G. Izant, E. Lazarides
semanticscholar   +1 more source

A Sprayable Nanoplatform Breaks the Vicious Cycle of Diabetic Wounds via Photoactivated Antioxidant and Drug Delivery

Advanced Science, EarlyView.
A sprayable near‐infrared‐activated nanoplatform incorporating MXene, ZnHCF nanozyme, and deferoxamine efficiently breaks the vicious cycle of diabetic wounds. Upon irradiation, interfacial electron transfer and photothermal effects enhance multi‐enzyme activity, enabling explosive ROS elimination, alleviation of hypoxia, and controlled DFO release ...
Jiahao Guo, Yuanyuan Meng, Qixiang Gui, Xi‐Tao Yang, Yong Fan, Xiaodong Zhu   +5 more
wiley   +1 more source

Analysis of proteomic differences between liquefied after-cataracts and normal lenses using two-dimensional gel electrophoresis and mass spectrometry. [PDF]

Int J Ophthalmol, 2017
Ge JJ, Huang YS.
europepmc   +1 more source

PDIA6–SCD1 Axis Rewires Lipid Metabolism to Drive Gastric Cancer Progression

Advanced Science, EarlyView.
Protein disulfide isomerase A6 (PDIA6) is identified as an oncogenic driver in gastric cancer. PDIA6 directly binds and stabilizes SCD1 by limiting its ubiquitin–proteasome‐mediated degradation, thereby sustaining monounsaturated fatty acid (MUFA)‐enriched lipid homeostasis and lipid metabolic reprogramming.
Zhen Tian, Yifan Cheng, Jiajie Zhou, Ruiqi Li, Shuai Zhao, Ben Li, Zijie Xu, Mengli Zi, Yayan Fu, Chenkai Zhang, Qiannan Sun, Shantanu Baral, Sen Wang, Daorong Wang   +13 more
wiley   +1 more source