Results 81 to 90 of about 19,604,431 (252)

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

FEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli, Mohammad Nour Alsamsam, Ugnė Bagdonaitė, Van Truc Vu, Chun‐Jen Huang, Polina Kuzhir, Mindaugas Zaremba, Aurimas Kopūstas, Marijonas Tutkus   +8 more
wiley   +1 more source

Rate-dependent extensions of the parametric magneto-dynamic model with magnetic hysteresis

AIP Advances, 2017
This paper extends the parametric magneto-dynamic model of soft magnetic steel sheets to account for the phase shift between local magnetic flux density and magnetic field strength.
S. Steentjes, M. Petrun, G. Glehn, D. Dolinar, K. Hameyer   +4 more
doaj   +1 more source

Analysing the significance of small conformational changes and low occupancy states in serial crystallographic data

FEBS Open Bio, EarlyView.
This protocol paper outlines methods to establish the success of a time‐resolved serial crystallographic experiment, by means of statistical analysis of timepoint data in reciprocal space and models in real space. We show how to amplify the signal from excited states to visualise structural changes in successful experiments.
Jake Hill, Yelyzaveta Pulnova, Elke De Zitter, Helen M. Ginn, Briony A. Yorke   +4 more
wiley   +1 more source

Solutions of the Mathieu–Hill Equation for a Trapped-Ion Harmonic Oscillator—A Qualitative Discussion

Mathematics
We investigate solutions of the classical Mathieu–Hill (MH) equation that characterizes the dynamics of trapped ions. The analytical model we introduce demonstrates the equations of motion are equivalent to those of a harmonic oscillator (HO).
Bogdan M. Mihalcea
doaj   +1 more source

Refinement of amino‐acid conformation vs. difference density maps in time‐resolved serial femtosecond crystallography data analysis

FEBS Open Bio, EarlyView.
The dFoCC pipeline starts with observed DED and resting‐state coordinates, which are then used to generate a library of triggered states. Correlation analysis of the calculated DED features of each candidate vs observed DED permits quantitative evaluation of candidate structural quality.
Meng Iao Fong, Yuhei Hosokawa, Lars Oliver Essen, Manuel Maestre‐Reyna   +3 more
wiley   +1 more source

Nonlinear Dynamic Behavior of a Flexible Structure to Combined External Acoustic and Parametric Excitation

Shock and Vibration, 2006
Flexible structures are frequently subjected to multiple inputs when in the field environment. The accurate determination of the system dynamic response to multiple inputs depends on how much information is available from the excitation sources that act ...
Paulo S. Varoto, Demian G. Silva
doaj   +1 more source

Directed evolution of enzymes at the crossroads of tradition and innovation

FEBS Open Bio, EarlyView.
An iterative cycle of data‐driven enzyme optimization comprising four stages: genetic diversification of a template enzyme, expression of protein variants, high‐throughput evaluation, and machine‐learning‐guided redesign of the next variant library.
Maria Tomkova, Andrej Mirossay, Erik Sedlak   +2 more
wiley   +1 more source

Hyperactive ice‐binding proteins stabilize cell membranes and improve resistance to dehydration stress in Caenorhabditis elegans

FEBS Open Bio, EarlyView.
TisIBP8, a fungal‐derived hyperactive ice‐binding protein, helps Caenorhabditis elegans survive dehydration. It localizes near cell membranes, reduces cell damage, and helps maintain membrane structure during drying. These results suggest that ice‐binding proteins can protect cells from dehydration stress as well as freezing stress.
Daiki Shimose, Kotaro Ozaki, Ryohei Kuriyama, Yuka Ikemoto, Kazuhiro Mio, Yuji C. Sasaki, Tatsuya Arai, Sakae Tsuda, Yoichi Shinkai, Masahiro Kuramochi   +9 more
wiley   +1 more source

Parametric transfer in a synchronously pumped optical parametric oscillator [PDF]

, 2006
The conditions for high fidelity parametric transfer of the pulse shape of a near-infrared pump pulse to a mid-infrared idler pulse of a synchronously pumped optical parametric oscillator are investigated theoretically and ...
Shepherd, D.P., Hanna, D.C., J. Prawiharjo, D.C. Hanna, Naz, N.A., Prawiharjo, J., D.P. Shepherd, N.A. Naz, Hung, H.S.S., H.S.S. Hung   +9 more
core   +1 more source

A minimized falling damage method for humanoid robots

International Journal of Advanced Robotic Systems, 2017
In order to better adapt to human living environment for improving the ability of serving people on various occasions, humanoid robots need to prevent themselves from being severely damaged during falling backward.
Qingqing Li, Xuechao Chen, Yuhang Zhou, Zhangguo Yu, Weimin Zhang, Qiang Huang   +5 more
doaj   +1 more source