Tandem Affinity Purification of SBP-CBP-tagged Type Three Secretion System Effectors [PDF]
Bio-Protocol, 2019 Identification of protein-protein interactions of bacterial effectors and cellular targets during infection is at the core to understand how bacteria manipulate the infected host to overcome the immune response.
Laura Berneking +3 more
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Interrelationship between type three secretion system and metabolism in pathogenic bacteria [PDF]
Frontiers in Cellular and Infection Microbiology, 2014 Before the advent of molecular biology methods, studies of pathogens were dominated by analyses of their metabolism. Development of molecular biology techniques then enabled the identification and functional characterisation of the fascinating toolbox of
Gottfried eWilharm, Christine eHeider
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PLoS ONE, 2010 BackgroundEnteropathogenic E. coli (EPEC) and enterohemorrhagic E. coli (EHEC) are two categories of E. coli strains associated with human disease. A major virulence factor of both pathotypes is the expression of a type three secretion system (TTSS ...
Mariano Larzábal +5 more
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Type three secretion system effector translocation: one step or two? [PDF]
Frontiers in Cellular and Infection Microbiology, 2011 Type three secretion systems (T3SSs) are macromolecular structures utilized by a number of bacterial pathogens to transfer proteins from the bacterial cytosol, across bacterial and host membranes, into host cells (Cornelis, 2006). The process of crossing the bacterial membranes is termed secretion with the steps of crossing the host membranes being ...
Charlotte A Perrett, Daoguo eZhou
doaj +4 more sources
Interfacial amino acids support Spa47 oligomerization and shigella type three secretion system activation. [PDF]
Proteins, 2019 AbstractLike many Gram‐negative pathogens, Shigella rely on a type three secretion system (T3SS) for injection of effector proteins directly into eukaryotic host cells to initiate and sustain infection. Protein secretion through the needle‐like type three secretion apparatus (T3SA) requires ATP hydrolysis by the T3SS ATPase Spa47, making it a likely ...
Demler HJ +5 more
europepmc +8 more sources
Chemical inhibitors of the type three secretion system: disarming bacterial pathogens. [PDF]
Antimicrob Agents Chemother, 2012 ABSTRACT The recent and dramatic rise of antibiotic resistance among bacterial pathogens underlies the fear that standard treatments for infectious disease will soon be largely ineffective. Resistance has evolved against nearly every clinically used antibiotic, and in the near future, we may be hard-pressed to treat bacterial infections ...
Duncan MC, Linington RG, Auerbuch V.
europepmc +7 more sources
Type three secretion system in non-toxigenic Vibrio cholerae O1, Mexico. [PDF]
J Med Microbiol, 2014 Cholera reemerged in Latin America in 1991, after being absent for more than 100 years (Sepulveda et al., 2006). Initially appearing in Peru in January, cholera reached Mexico in June, affecting 45 977 people in Mexico alone until 2002 (Sepulveda et al., 2006).
Mahmud J +6 more
europepmc +5 more sources
Structural and Biophysical Characterization of the Yersinia Type Three Secretion System ATPase YscN. [PDF]
ProteinsBarker SA +4 more
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HexR Transcription Factor Contributes to Pseudomonas cannabina pv. alisalensis Virulence by Coordinating Type Three Secretion System Genes [PDF]
Microorganisms, 2023 Pseudomonas cannabina pv. alisalensis (Pcal) causes bacterial blight on cabbage. We previously conducted a screening for reduced virulence using Tn5 transposon mutants and identified one of the transcriptional factors, HexR, as a potential Pcal virulence
Nanami Sakata +5 more
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Novel compounds targeting the enterohemorrhagic Escherichia coli type three secretion system reveal insights into mechanisms of secretion inhibition. [PDF]
Mol Microbiol, 2017 SummaryAnti‐virulence (AV) compounds are a promising alternative to traditional antibiotics for fighting bacterial infections. The Type Three Secretion System (T3SS) is a well‐studied and attractive AV target, given that it is widespread in more than 25 species of Gram‐negative bacteria, including enterohemorrhagic E.
Zambelloni R +5 more
europepmc +8 more sources