Results 121 to 130 of about 10,954 (152)

Protein-RNA interactions in 20S U5 snRNPs

Biochimica et Biophysica Acta (BBA) - Gene Structure and Expression, 1991
The interaction of the U5-specific polypeptides with U5 snRNA was investigated by comparison of the differential accessibility towards nucleases and dimethylsulfate of defined regions of U5 snRNA in purified 20S and 10S U5 snRNPs. While 20S U5 snRNPs contain eight U5-specific proteins in addition to the common proteins, the 10S U5 snRNPs contain only ...
M, Bach, R, Lührmann
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Roles of the U5 snRNP in spliceosome dynamics and catalysis

Biochemical Society Transactions, 2004
Most protein-coding genes in eukaryotes are interrupted by non-coding intervening sequences (introns), which must be precisely removed from primary gene transcripts (pre-mRNAs) before translation of the message into protein. Intron removal by pre-mRNA splicing occurs in the nucleus and is catalysed by complex ribonucleoprotein machines called ...
I A, Turner, C M, Norman, M J, Churcher, A J, Newman   +3 more
openaire   +2 more sources

Identification, characterization and crystal structure analysis of the human spliceosomal U5 snRNP-specific 15 kd protein

Journal of Molecular Biology, 1999
The U5 small ribonucleoprotein particle (snRNP) contains various proteins involved in catalytic activities mediating conformational rearrangements of the spliceosome.
Ralf Ficner, Reinhard Georg Lührmann, Klaus Reuter   +2 more
exaly   +2 more sources

Isolation of U5 snRNP particles.

Cytobios, 1989
A method for the isolation of intact U5 small nuclear RNP particles from HeLa cells has been developed. The procedure includes nuclear extraction of the particles at moderate ionic strength, fractionation in agarose gels, electrophoretic transfer on DEAE cellulose paper and elution with ammonium chloride.
Pironcheva, G., Zhelev, N., Russev, G.
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A U1/U4/U5 snRNP Complex Induced by a 2′- O -Methyl-Oligoribonucleotide Complementary to U5 snRNA

Science, 1996
Nuclear messenger RNA splicing involves multiple interactions between the five spliceosomal small nuclear ribonucleoprotein particles (snRNPs) U1, U2, U4, U5, and U6 and numerous spliceosomal proteins. Here it is shown that binding of a 2′- O -methyl-oligoribonucleotide complementary to U5 small nuclear RNA (snRNA ...
G, Ast, A M, Weiner
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U5 snRNP particles bind pre-messenger RNA in vitro.

Cytobios, 1989
The ability of purified U5 small nuclear RNP particles (U5 snRNPs) to bind in vitro pre-mRNA of the human beta-globin gene was investigated. The transcript which contained sequences, corresponding to the second intron and fragments of the second and third exons of the gene was bound to U5 snRNP particles in the presence of antibodies specific against ...
G L, Pironcheva, G, Russev
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Phylogenic Analysis of Cellular Life Forms Using U5 Snrnp Components

Journal of Applied Bioinformatics & Computational Biology, 2013
Phylogenic Analysis of Cellular Life Forms Using U5 Snrnp Components The conserved sequences of certain molecules in existing cellular life forms, like those reside in RNA splicing, are suitable for phylogenetic analysis. In view of the growing interests in the field of primate’s evolution, protein components of the U5 snRNP were analyzed using NCBI ...
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Study into a role for Aar2p in U5 snRNP biogenesis

2014
Aar2p is an essential yeast protein involved in pre-mRNA splicing and component of a U5 snRNP precursor form. It has been suggested that the mature U5 snRNP and U4/U6.U5 tri-snRNP assemble from the Aar2p-U5 core particle that is formed in the cytoplasm.
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U1 snRNP increases RNA Pol II elongation rate to enable synthesis of long genes

Molecular Cell, 2023
Karen Adelman, Claudia A Mimoso
exaly  

The yeast Prp3 protein is a U4/U6 snRNP protein necessary for integrity of the U4/U6 snRNP and the U4/U6.U5 tri-snRNP.

RNA (New York, N.Y.), 1997
Previously, yeast prp3 mutants were found to be blocked prior to the first catalytic step of pre-mRNA splicing. No splicing intermediates or products are formed from pre-mRNA in heat-inactivated prp3 mutants or prp3 mutant extracts. Here we show that Prp3p is a component of the U4/U6 snRNP and is also present in the U4/U6.U5 tri-snRNP.
J G, Anthony, E M, Weidenhammer, J L, Woolford   +2 more
openaire   +1 more source