Characterization of ubiquitin-activating enzyme Uba1 in the nucleus by its mammalian temperature-sensitive mutant. [PDF]
PLoS ONE, 2014 Temperature-sensitive (ts) CHO-K1 mutant tsTM3 exhibits chromosomal instability and cell-cycle arrest in the S to G2 phases with decreased DNA synthesis at the nonpermissive temperature, 39°C.
Kimihiko Sugaya +4 more
doaj +1 more source
An adaptive stress response that confers cellular resilience to decreased ubiquitination
Nature Communications, 2023 Ubiquitination is a post-translational modification initiated by the E1 enzyme UBA1, which transfers ubiquitin to ~35 E2 ubiquitin-conjugating enzymes. While UBA1 loss is cell lethal, it remains unknown how partial reduction in UBA1 activity is endured ...
Liam C. Hunt +16 more
doaj +1 more source
Metabolic and Chaperone Gene Loss Marks the Origin of Animals: Evidence for Hsp104 and Hsp78 Sharing Mitochondrial Clients [PDF]
, 2015 The evolution of animals involved acquisition of an emergent gene repertoire for gastrulation. Whether loss of genes also co-evolved with this developmental reprogramming has not yet been addressed.
Erives, Albert, Fassler, Jan
core +9 more sources
Evolution of the ubiquitin-activating enzyme Uba1 (E1) [PDF]
Physica A: Statistical Mechanics and its Applications, 2017 Ubiquitin tags diseased proteins and initiates an enzyme conjugation cascade, which has three stages. The first-stage enzyme Uba1 (E1) has evolved only modestly from slime mold to humans, and is > 14 times larger than Ub. Here we use critical point thermodynamic scaling theory to connect Uba1 (E1) evolution from yeast and slime mold to fruit flies ...
Allan, Douglas C., Phillips, J. C.
openaire +2 more sources
Dengue Virus Genome Uncoating Requires Ubiquitination [PDF]
, 2016 The process of genome release or uncoating after viral entry is one of the least-studied steps in the flavivirus life cycle. Flaviviruses are mainly arthropod-borne viruses, including emerging and reemerging pathogens such as dengue, Zika, and West Nile ...
Byk, Laura Andrea +5 more
core +7 more sources
Acetylated Ubiquitin Modulates the Catalytic Activity of the E1 Enzyme Uba1 [PDF]
Biochemistry, 2021 Ubiquitin (Ub) signaling requires the covalent passage of Ub among E1, E2, and E3 enzymes. The choice of E2 and E3 enzymes combined with multiple rounds of the cascade leads to the formation of polyubiquitin chains linked through any one of the seven lysines on Ub. The linkage type and length act as a signal to trigger important cellular processes such
Rachel E. Lacoursiere, Gary S. Shaw
openaire +2 more sources
VEXAS syndrome in myelodysplastic syndrome with autoimmune disorder
Experimental Hematology & Oncology, 2021 VEXAS (vacuoles, E1 enzyme, X-linked, autoinflammatory, somatic) syndrome is a newly-described adult-onset inflammatory syndrome characterized by vacuoles in myeloid and erythroid precursor cells and somatic mutations affecting methionine-41 (p.Met41) in
Huijun Huang +12 more
doaj +1 more source
Novel somatic mutations in UBA1 as a cause of VEXAS syndrome [PDF]
Blood, 2021 Poulter and colleagues describe a series from the United Kingdom of 10 male patients with VEXAS syndrome, including 2 with novel genetic changes affecting methionine 41 of E1.
James A. Poulter +14 more
openaire +3 more sources
Hemi-Orolingual Angioedema in a Patient With VEXAS Syndrome. [PDF]
EJHaemeJHaem, Volume 7, Issue 1, February 2026.
Suárez EU +2 more
europepmc +2 more sources
A Conditional Yeast E1 Mutant Blocks the Ubiquitin–Proteasome Pathway and Reveals a Role for Ubiquitin Conjugates in Targeting Rad23 to the Proteasome [PDF]
, 2007 E1 ubiquitin activating enzyme catalyzes the initial step in all ubiquitin-dependent processes. We report the isolation of uba1-204, a temperature-sensitive allele of the essential Saccharomyces cerevisiae E1 gene, UBA1.
Amerik A. Y. +59 more
core +2 more sources