Results 51 to 60 of about 27,627 (205)

Redirecting the specificity of ubiquitination by modifying ubiquitin-conjugating enzymes. [PDF]

Proceedings of the National Academy of Sciences, 1995
Depletion of specific cellular proteins is a powerful tool in biological research and has many medical and agricultural benefits. In contrast to genetic methods currently available to attenuate protein levels, we describe an alternative approach that redirects the ubiquitin-dependent proteolytic pathway to facilitate specific proteolytic removal ...
Mark Gosink, Richard D. Vierstra
openaire   +3 more sources

The E2 Ubiquitin-conjugating Enzyme UBE2J1 Is Required for Spermiogenesis in Mice [PDF]

Journal of Biological Chemistry, 2014
ER-resident proteins destined for degradation are dislocated into the cytosol by components of the ER quality control machinery for proteasomal degradation. Dislocation substrates are ubiquitylated in the cytosol by E2 ubiquitin-conjugating/E3 ligase complexes. UBE2J1 is one of the well-characterized E2 enzymes that participate in this process. However,
Koenig, Paul-Albert, Hagiwara, Masatoshi, Maruyama, Takeshi, Watson, Nicki, Page, David C, Nicholls, Peter, Schmidt, Florian Ingo, Ploegh, Hidde, Frydman, Galit H.   +8 more
openaire   +5 more sources

Activity-based protein profiling reveals both canonical and novel ubiquitin pathway enzymes in Plasmodium.

PLoS Pathogens
The ubiquitin-proteasome system (UPS) is essential for Plasmodium falciparum survival and represents a potential target for antimalarial therapies. We utilised a ubiquitin- activity based probe (Ub-Dha) to capture active components of the ubiquitin ...
Cameron Smith, Mohsen Hajisadeghian, Gerbrand J van der Heden van Noort, Michael J Deery, Adán Pinto-Fernández, Benedikt M Kessler, Katerina Artavanis-Tsakonas   +6 more
doaj   +1 more source

Proteasome inhibitors: new class of antitumor agents

Biomolecules & Biomedicine, 2003
The ubiquitin-proteasome pathway is the principal pathway for intracellular protein degradation1,2 (Fig 1). This pathway selectively degrades an extensive number of short-lived regulatory proteins involved in the control of normal cellular processes. In
Gordan Srkalović
doaj   +1 more source

Optimization of collective enzyme activity via spatial localization [PDF]

J. Chem. Phys. 139, 135101 (2013), 2013
The spatial organization of enzymes often plays a crucial role in the functionality and efficiency of enzymatic pathways. To fully understand the design and operation of enzymatic pathways, it is therefore crucial to understand how the relative arrangement of enzymes affects pathway function. Here we investigate the effect of enzyme localization on the
arxiv   +1 more source

A conserved asparagine has a structural role in ubiquitin-conjugating enzymes [PDF]

Nature Chemical Biology, 2013
It is widely accepted that ubiquitin-conjugating enzymes contain an active site asparagine that serves as an oxyanion hole, thereby stabilizing a negatively charged transition state intermediate and promoting ubiquitin transfer. Using structural and biochemical approaches to study the role of the conserved asparagine to ubiquitin conjugation by Ubc13 ...
Ian Yu, Alison E. Ringel, Reuven Wiener, Cynthia Wolberger, Christopher E. Berndsen, Christopher E. Berndsen   +5 more
openaire   +3 more sources

Orthogonal ubiquitin transfer identifies ubiquitination substrates under differential control by the two ubiquitin activating enzymes

Nature Communications, 2017
The transfer of ubiquitin (UB) to cellular targets is mediated sequentially by three groups of enzymes, UB activating enzyme (E1), UB conjugating enzyme (E2) and UB ligase (E3).
Xianpeng Liu, Bo Zhao, Limin Sun, Karan Bhuripanyo, Yiyang Wang, Yingtao Bi, Ramana V. Davuluri, Duc M. Duong, Dhaval Nanavati, Jun Yin, Hiroaki Kiyokawa   +10 more
doaj   +1 more source

Ubiquitin control of S phase: a new role for the ubiquitin conjugating enzyme, UbcH7

Cell Division, 2009
Events within and transitions between the phases of the eukaryotic cell cycle are tightly controlled by transcriptional and post-translational processes. Prominent among them is a profound role for the ubiquitin proteasome proteolytic pathway. The timely
Taylor Allen, Whitcomb Elizabeth A
doaj   +1 more source

UBE2S promotes cell chemoresistance through PTEN-AKT signaling in hepatocellular carcinoma

Cell Death Discovery, 2021
Ubiquitination displays a crucial role in various biological functions, such as protein degradation, signal transduction, and cellular homeostasis. Accumulating evidence has indicated that ubiquitination is essential in cancer progression.
Liang Gui, Sicai Zhang, Yongzi Xu, Hongwei Zhang, Ying Zhu, Lianbao Kong   +5 more
doaj   +1 more source

Information propagation in time through allosteric signaling [PDF]

Phys. Rev. Research 2, 023367 (2020), 2019
Naively, one expects the information communicated by an enzyme downstream within a signaling network, in which the enzyme is embedded, to grow monotonically with the enzyme's rate of product formation. However, here we observe that this does not necessarily hold true for allosterically regulated enzymes, often observed in signaling networks.
arxiv   +1 more source