Results 101 to 110 of about 63,885 (332)

A human ubiquitin-conjugating enzyme homologous to yeast UBC8.

Journal of Biological Chemistry, 1994
Ubiquitin-conjugating enzymes catalyze the covalent attachment of ubiquitin to cellular substrates. Here we describe the isolation of a novel ubiquitin-conjugating enzyme from human placenta and the cloning of the corresponding cDNA. DNA sequencing revealed that this gene, UbcH2, encodes a protein with significant sequence similarity to yeast UBC8.
Peter K. Kaiser, L. Hofferer, C Sachsenmaier, Rainer Schneider, Barbara Kofler, Wolfgang Seufert, Stefan Jentsch, Herbert Herzog, Manfred Schweiger   +8 more
openaire   +3 more sources

A Ubiquitin-Conjugating Enzyme Is Essential for Developmental Transitions inDictyostelium [PDF]

Molecular Biology of the Cell, 1997
We have identified a developmentally essential gene,UbcB, by insertional mutagenesis. The encoded protein (UBC1) shows very high amino acid sequence identity to ubiquitin-conjugating enzymes from other organisms, suggesting that UBC1 is involved in protein ubiquitination and possibly degradation during Dictyostelium development.
Anson Nomura, Sudhasri Mohanty, Richard A. Firtel, Alexandra L. Clark   +3 more
openaire   +3 more sources

Enhancing Specificity, Precision, Accessibility, Flexibility, and Safety to Overcome Traditional CRISPR/Cas Editing Challenges and Shape Future Innovations

Advanced Science, EarlyView.
CRISPR/Cas9, while transformative, faces challenges in specificity, precision, delivery, accessibility, flexibility, and safety. This review addresses these limitations by highlighting strategies to reduce off‐target effects, exploring HDR‐based and alternative editing approaches, and evaluating advanced delivery mechanisms.
Muna Alariqi, Mohamed Ramadan, Lu Yu, Fengjiao Hui, Amjad Hussain, Xiaofeng Zhou, Yu Yu, Xianlong Zhang, Shuangxia Jin   +8 more
wiley   +1 more source

Ubiquitylation in ERAD: reversing to go forward? [PDF]

PLoS Biology, 2011
Proteins are co-translationally inserted into the endoplasmic reticulum (ER) where they undergo maturation. Homeostasis in the ER requires a highly sensitive and selective means of quality control.
Yien Che Tsai, Allan M Weissman
doaj   +1 more source

The E2 ubiquitin-conjugating enzymes, AtUBC1 and AtUBC2, play redundant roles and are involved in activation of FLC expression and repression of flowering in Arabidopsis thaliana.

The Plant Journal, 2009
Post-translational modifications of proteins by addition of ubiquitin can regulate protein degradation and localization, protein-protein interactions and transcriptional activation.
Lin Xu, R. Ménard, A. Berr, J. Fuchs, V. Cognat, D. Meyer, W. Shen   +6 more
semanticscholar   +1 more source

Three-dimensional structure of a ubiquitin-conjugating enzyme (E2).

Journal of Biological Chemistry, 1992
The x-ray crystal structure of a recombinant ubiquitin-conjugating enzyme (E2) encoded by the UBC1 gene of the plant Arabidopsis thaliana has been determined with the use of multiple isomorphous replacement techniques and refined at 2.4-A resolution by simulated annealing and restrained least-squares.
R.D. Vierstra, M.L. Sullivan, Leigh C. Jeffrey, William J. Cook   +3 more
openaire   +3 more sources

Post‐Translational Modifications in Cilia and Ciliopathies

Advanced Science, EarlyView.
This review synthesizes current understanding of post‐translational modifications (PTMs) in ciliary proteins and emphasizes their roles in ciliary formation, homeostasis, and signaling. This review also discusses the implication of PTM dysregulation in ciliopathies and explores therapeutic strategies targeting PTM‐modifying enzymes.
Jie Ran, Jun Zhou
wiley   +1 more source

Rictor Ameliorates Acute Antibody‐Mediated Rejection Following Kidney Transplantation by Suppressing Macrophage M1 Polarization Through p65‐NLRP3 Axis

Advanced Science, EarlyView.
This study elucidates the critical role of Rictor in macrophage activation in acute antibody‐mediated rejection (ABMR). Rictor increases K48‐linked ubiquitination of p65 by upregulating E3 ubiquitin ligase SOCS1, inhibiting transcriptional levels of NLRP3 and inflammasome activation.
Bin Ni, Chengcheng Yang, Junqi Zhang, Zhou Hang, Ming Zheng, Dengyuan Feng, Qinghuan Shen, Jinxu Miao, Xulin Sun, Li Sun, Baixin Shen, Min Gu, Zijie Wang   +12 more
wiley   +1 more source

In vitro Ubiquitin Dimer Formation Assay

Bio-Protocol, 2017
The process of protein ubiquitination typically consists of three sequential steps to add an ubiquitin (Ub) or Ub chain to a substrate protein, requiring three different enzymes, ubiquitin activating enzyme (E1), ubiquitin conjugating enzyme (E2), and ...
Sheng Wang, Ling Cao, Hong Wang
doaj   +1 more source

Structural analysis of recombinant human ubiquitin-conjugating enzyme UbcH5c

Acta Pharmaceutica Sinica B, 2017
UbcH5c belongs to the ubiquitin-conjugating enzyme family and plays an important role in catalyzing ubiquitination during TNF-α--triggered NF-κB activation. Therefore, UbcH5c is a potent therapeutic target for the treatment of inflammatory and autoimmune diseases induced by aberrant activation of NF-κB. In this study, we established a stable expression
Honglin Li, Junsheng Zhu, Fangshu Wu, Lili Zhu   +3 more
openaire   +3 more sources