Results 71 to 80 of about 39,918 (341)

RING fingers mediate ubiquitin-conjugating enzyme (E2)-dependent ubiquitination [PDF]

Proceedings of the National Academy of Sciences, 1999
A RING finger-containing protein (AO7) that binds ubiquitin-conjugating enzymes (E2s) and is a substrate for E2-dependent ubiquitination was identified. Mutations of cation-coordinating residues within AO7’s RING finger abolished ubiquitination, as did chelation of zinc.
K L, Lorick, J P, Jensen, S, Fang, A M, Ong, S, Hatakeyama, A M, Weissman   +5 more
openaire   +2 more sources

Genetic attenuation of ALDH1A1 increases metastatic potential and aggressiveness in colorectal cancer

Molecular Oncology, EarlyView.
Aldehyde dehydrogenase 1A1 (ALDH1A1) is a cancer stem cell marker in several malignancies. We established a novel epithelial cell line from rectal adenocarcinoma with unique overexpression of this enzyme. Genetic attenuation of ALDH1A1 led to increased invasive capacity and metastatic potential, the inhibition of proliferation activity, and ultimately ...
Martina Poturnajova, Zuzana Kozovska, Ondrej Pos, Kristina Pavlov, Sachin Gulati, Peter Makovicky, Kristina Jakic, Monika Burikova, Eva Sedlackova, Barbora Svitkova, Silvia Tyciakova, Vojtech Bystry, Nicolas Blavet, Boris Tichy, Matej Hrnciar, Jaroslav Budis, Miroslav Tomas, Peter Dubovan, Georgina Kolnikova, Veronika Repaska, Nikoleta Mojzesova, Eva Zomborska, Daniel Pindak, Michal Mego, Tomas Szemes, Miroslava Matuskova   +25 more
wiley   +1 more source

Targeting the ubiquitin system by fragment-based drug discovery

Frontiers in Molecular Biosciences, 2022
The ubiquitin system contains a wealth of potential drug targets for many diseases and conditions, including neurodegenerative, immune, metabolic and developmental diseases, as well as multiple cancers.
Cassandra Kennedy, Katherine McPhie, Katrin Rittinger   +2 more
doaj   +1 more source

Meta‐analysis fails to show any correlation between protein abundance and ubiquitination changes

FEBS Open Bio, EarlyView.
We analyzed over 50 published proteomics datasets to explore the relationship between protein levels and ubiquitination changes across multiple experimental conditions and biological systems. Although ubiquitination is often associated with protein degradation, our analysis shows that changes in ubiquitination do not globally correlate with changes in ...
Nerea Osinalde, Unai Alduntzin, Gorka Prieto, Ugo Mayor   +3 more
wiley   +1 more source

The role of ubiquitin-conjugating enzyme in the process of spermatogenesis

Reproductive Biology and Endocrinology
The ubiquitination is crucial for controlling cellular homeostasis and protein modification, in which ubiquitin-conjugating enzyme (E2) acts as the central player in the ubiquitination system.
Peng Lv, Jihong Liu, Xiaming Liu
doaj   +1 more source

Structural basis for the RING catalyzed synthesis of K63 linked ubiquitin chains [PDF]

, 2015
This work was supported by grants from Cancer Research UK (C434/A13067), the Wellcome Trust (098391/Z/12/Z) and Biotechnology and Biological Sciences Research Council (BB/J016004/1).The RING E3 ligase catalysed formation of lysine 63 linked ubiquitin ...
A Plechanovová, A Plechanovová, A Rojas-Fernandez, AA Yunus, AJ McCoy, Anna Plechanovová, AP VanDemark, CE Berndsen, CE Berndsen, CM Guzzo, D Reverter, DC Scott, E Krissinel, Ellis G Jaffray, Emma Branigan, G Winter, GN Murshudov, H Dou, James H Naismith, JN Pruneda, L Chang, L Spyracopoulos, M Scheffner, M Zhang, MB Metzger, MD Winn, MH Tatham, MH Tatham, MJ Eddins, MJ Lewis, P Emsley, P Evans, PY Wu, R Vyas, RM Hofmann, Ronald T Hay, S McKenna, S McKenna, TF Moraes, VB Chen, W Kabsch, Y Galanty, Y Yin   +42 more
core   +4 more sources

Triazine‐Trione Thermosets with High Processability for Scaffold Applications in Bone Tissue Engineering

Advanced Healthcare Materials, EarlyView.
Novel photo‐clickable triazine‐trione thermosets can be shaped and cured under mild conditions, including room and physiological temperatures. These materials are biocompatible and support osteogenic differentiation of bone marrow–derived mesenchymal stem cells on their surface.
Åshild Johansen, Shuntaro Yamada, Daniel J. Hutchinson, Mohammed A. Yassin, Samih Mohamed‐Ahmed, Cecilie Gjerde, Michael Malkoch, Kamal Mustafa   +7 more
wiley   +1 more source

Put a RING on it: Regulation and inhibition of RNF8 and RNF168 RING finger E3 ligases at DNA damage sites.

Frontiers in Genetics, 2013
RING domain containing E3 ubiquitin ligases comprise a large family of enzymes that in combination with an E2 ubiquitin-conjugating enzyme, modify target proteins by attaching ubiquitin moieties.
Cristina eBartocci, Eros eLazzerini Denchi   +1 more
doaj   +1 more source

The anti-inflammatory drug BAY 11-7082 suppresses the MyD88-dependent signalling network by targeting the ubiquitin system [PDF]

, 2013
The compound BAY 11-7082 inhibits IκBα [inhibitor of NF-κB (nuclear factor κB)α] phosphorylation in cells and has been used to implicate the canonical IKKs (IκB kinases) and NF-κB in >350 publications. In the present study we report that BAY 11-7082 does
Axel Knebel, Christoph H. Emmerich, Clark, Clark, David, David G. Campbell, Deng, Ea, Geoghegan, Greer, Juliana, Kanayama, Kensche, Kim, Kirisako, Komander, Lang, Lin, Lorna Plater, Maria Stella Ritorto, Martin, Meng, Mori, Motshwene, Nanda, Natalia Shpiro, Ngo, Ninomiya-Tsuji, Padwa, Panier, Pauls, Philip Cohen, Pierce, Pulvino, Rahighi, Rahighi, Sam Strickson, Sato, Scaife, Shim, Smit, Soucy, Tokunaga, Walczak, Wang, Wang, Waterfield, Wenzel, Windheim, Wu, Xia   +50 more
core   +4 more sources

Autoinhibition Mechanism of the Ubiquitin-Conjugating Enzyme UBE2S by Autoubiquitination [PDF]

Structure, 2019
Ubiquitin-conjugating enzymes (E2s) govern key aspects of ubiquitin signaling. Emerging evidence suggests that the activities of E2s are modulated by posttranslational modifications; the structural underpinnings, however, are largely unclear. Here, we unravel the structural basis and mechanistic consequences of a conserved autoubiquitination event near
Liess, AKL, Kucerova, A, Schweimer, K, Yu, L, Roumeliotis, TI, Diebold, M, Dybkov, O, Sotriffer, C, Urlaub, H, Choudhary, JS, Mansfeld, J, Lorenz, S   +11 more
openaire   +6 more sources