Results 41 to 50 of about 33,702 (335)
Frontiers in Microbiology, 2018 Ubiquitination is an essential protein modification in eukaryotic cells, which is reversible. Deubiquitinating enzymes (DUBs) catalyze deubiquitination process to reverse ubiquitination, maintain ubiquitin homeostasis or promote protein degradation by ...
Zhao Wang +5 more
doaj +1 more source
Multimodal activation of the ubiquitin ligase SCF by Nedd8 conjugation [PDF]
, 2008 Conjugation of ubiquitin-like protein Nedd8 to cullins (neddylation) is essential for the function of cullin-RING ubiquitin ligases (CRLs). Here, we show that neddylation stimulates CRL activity by multiple mechanisms.
Deshaies, Raymond J., Saha, Anjanabha
core +1 more source
Ubiquitin- and ATP-dependent unfoldase activity of P97/VCP•NPLOC4•UFD1L is enhanced by a mutation that causes multisystem proteinopathy [PDF]
, 2017 p97 is a “segregase” that plays a key role in numerous ubiquitin (Ub)-dependent pathways such as ER-associated degradation. It has been hypothesized that p97 extracts proteins from membranes or macromolecular complexes to enable their proteasomal ...
Blythe, Emily E. +3 more
core +2 more sources
, 2011 In this chapter, we describe a method for detecting the ubiquitination status of G protein-coupled receptors (GPCRs). This involves co-expression of a GPCR with an epitope-tagged ubiquitin construct in a -heterologous mammalian expression system. Stimulus-dependent modification of the GPCR by -ubiquitin is detected by immunoprecipitation and subsequent
Adriano Marchese, Adriana Caballero
openaire +3 more sources
Using Ubiquitin Binders to Decipher the Ubiquitin Code [PDF]
Trends in Biochemical Sciences, 2019 Post-translational modifications (PTMs) by ubiquitin (Ub) are versatile, highly dynamic, and involved in nearly all aspects of eukaryote biological function. The reversibility and heterogeneity of Ub chains attached to protein substrates have complicated their isolation, quantification, and characterization.
Mattern M +4 more
openaire +3 more sources
Frontiers in Plant Science, 2017 Drought stress from soil or air limits plant growth and development, leading to a reduction in crop productivity. Several E3 ligases positively or negatively regulate the drought stress response.
Sang-Wook Han +3 more
doaj +1 more source
Mechanism of Lysine 48 Selectivity during Polyubiquitin Chain Formation by the Ube2R1/2 Ubiquitin-Conjugating Enzyme [PDF]
, 2016 Lysine selectivity is of critical importance during polyubiquitin chain formation because the identity of the lysine controls the biological outcome. Ubiquitins are covalently linked in polyubiquitin chains through one of seven lysine residues on its ...
Harrison, Joseph S. +4 more
core +5 more sources
Rapid E2-E3 Assembly and Disassembly Enable Processive Ubiquitylation of Cullin-RING Ubiquitin Ligase Substrates [PDF]
, 2009 Degradation by the ubiquitin-proteasome system requires assembly of a polyubiquitin chain upon substrate. However, the structural and mechanistic features that enable template-independent processive chain synthesis are unknown.
Deshaies, Raymond J. +4 more
core +3 more sources
A Ubiquitin-like Protein Unleashes Ubiquitin Ligases [PDF]
Cell, 2008 Modification of cullin-RING ubiquitin ligases by the ubiquitin-like molecule Nedd8 promotes substrate ubiquitination. A crystal structure of a cullin modified by Nedd8 recently reported in Cell (Duda et al., 2008) and a biochemical study in Molecular Cell (Saha and Deshaies, 2008) reveal the dramatic impact on the ligase machinery by conjugation of ...
Ning Zheng +2 more
openaire +3 more sources
International Journal of Molecular Sciences, 2018 Ever since the discovery of ubiquitin in 1975[...]
openaire +2 more sources